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Sequencing HEteRo RNA-DNA-hYbrid (SHERRY). Impact on size profile is much harder to notice compared with DNA/DNA tagmentation (Figs S9 and S10).

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Tn5 tagmentation with custom adapters ending with T7 promoters, followed by linear amplification by transcription, followed by cDNA synthesis and sequencing.

Nat Genet. 2014 Dec;46(12):1343-9. doi:10.1038/ng.3119

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The Tn5 transposase still binds DNA after ligating its payload.

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Barcodes provided in a third molecule, integrated by PCR assembly.

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Tn5 is not efficient on hyperbranched DNA. Random hexamers and DNA polymerase I were used to debranch the DNA.