Dernières modifications :

Café
diff --git a/biblio/30272001.mdwn b/biblio/30272001.mdwn
new file mode 100644
index 0000000..af5fe9c
--- /dev/null
+++ b/biblio/30272001.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Diatom bloom-derived biotoxins cause aberrant development and gene expression in the appendicularian chordate Oikopleura dioica"]]
+[[!tag Oikopleura]]
+
+Commun Biol. 2018 Aug 24;1:121. doi:10.1038/s42003-018-0127-2
+
+Torres-Águila NP, Martí-Solans J, Ferrández-Roldán A, Almazán A, Roncalli V, D'Aniello S, Romano G, Palumbo A, Albalat R, Cañestro C.
+
+Diatom bloom-derived biotoxins cause aberrant development and gene expression in the appendicularian chordate Oikopleura dioica
+
+[[!pmid 30272001 desc="Polyunsaturated aldehydes released by diatoms impair the development of O. dioica by delaying the onset of expression of developmental genes.  Cell proliferation is not affected and “defensome” genes such as Aldh genes or Gclm (Glutamate-cysteine ligase modifier) are induced by the toxins.  Chaetoceros calcitrans, a diatom used as food when culturing O. dioica, is also shown to have a toxic effect."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 5467f6e..f074d6f 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -25,6 +25,9 @@ Some links:
 Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
 µm in size), _Tetraselmis suecica_ (9.5 µm), and the chlorophyte _Chlorella
 sp._ (3.5 µm) [[Acuña and Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608]].
+The diatom Chaetoceros calcitrans, often used as a food, can be toxic at high
+concentrations, probably because of the production of biotoxins
+([[Torres-Águila and coll., 2018|biblio/30272001]]).
 
 Parasites: _Oodinium pouchetii_ and others.
 
@@ -124,6 +127,9 @@ Genes and pathways
    tail movements at larval and adult stages.
  - No olfactory receptors have been found in _Oikopleura_ nor in _Ciona_
    ([[Niimura, 2009|biblio/20333175]]).
+ - Defensome genes such as dehydrogenases and  (Glutamate-cysteine ligase modifier subunit; Gclm)
+   are activated by polyunsaturated aldehydes produced by diatoms
+   ([[Torres-Águila and coll., 2018|biblio/30272001]]).
 
 Transcriptome
 -------------
@@ -193,7 +199,8 @@ Development
    experiments indicate that the posterior portion of the tail is required for
    posterior migration of subchordal cell precursors ([[Kishi and coll,
    2014|biblio/25224225]]).
-
+ - Expression of development genes is retarded by polyunsaturated aldehydes produced
+   by diatoms ([[Torres-Águila and coll., 2018|biblio/30272001]]).
 
 Anatomy
 -------

creating tag page tags/OIST
diff --git a/tags/OIST.mdwn b/tags/OIST.mdwn
new file mode 100644
index 0000000..67c6b88
--- /dev/null
+++ b/tags/OIST.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged OIST"]]
+
+[[!inline pages="tagged(OIST)" actions="no" archive="yes"
+feedshow=10]]

Café
diff --git a/biblio/10.1007_978-4-431-54865-2_51.mdwn b/biblio/10.1007_978-4-431-54865-2_51.mdwn
new file mode 100644
index 0000000..2cd5d3b
--- /dev/null
+++ b/biblio/10.1007_978-4-431-54865-2_51.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="The Gelatinous Macroplankton Community at the Hatoma Knoll Hydrothermal Vent"]]
+[[!tag Oikopleura OIST]]
+
+Dhugal Lindsay, Mitsuko Umetsu, Mary Grossmann, Hiroshi Miyake, Hiroyuki Yamamoto
+
+Subseafloor Biosphere Linked to Hydrothermal Systems pp 639-666, 08 November 2014
+
+The Gelatinous Macroplankton Community at the Hatoma Knoll Hydrothermal Vent
+
+[[!doi 10.1007/978-4-431-54865-2_51 desc="Oikopeuridae found in the deep sea.  One Bathochordaeus had a ~2m-big house."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 8b996e7..5467f6e 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -262,5 +262,8 @@ Ecology
  - Carbon output of _O. dioica_ is either their house or fecal pellets, the
    ratio of which may depend on food concentration ([[Acuña and
    Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608.mdwn]]).
+ - Oikopleuridae can be found in the deep see.  For instance, [[Lindsay and
+   coll., 2014|biblio/10.1007_978-4-431-54865-2_51]] reported _Oikopleura_, _Mesochordaeus_
+   and _Bathochordaeus_ individuals in the Hatoma Knoll hydrothermal vent, Okinawa Trough.
 
 [[!inline pages="tagged(Oikopleura)" actions="no" limit=0]]

Minor.
diff --git a/biblio/10.1186_s41200-016-0075-9.mdwn b/biblio/10.1186_s41200-016-0075-9.mdwn
index a2e1fc1..b1f373e 100644
--- a/biblio/10.1186_s41200-016-0075-9.mdwn
+++ b/biblio/10.1186_s41200-016-0075-9.mdwn
@@ -5,6 +5,6 @@ R. E. Sherlock, K. R. Walz and B. H. Robison
 
 Marine Biodiversity Records 2016 9:79 doi:10.1186/s41200-016-0075-9
 
-The first definitive record of the giant larvacean, Bathochordaeus charon, since its original description in 1900 and a range extension to the northeast Pacific Ocean
+The first definitive record of the giant larvacean, _Bathochordaeus charon_, since its original description in 1900 and a range extension to the northeast Pacific Ocean
 
-[[!doi 10.1186/s41200-016-0075-9 desc="“In over two decades of exploring the mesopelagic waters of Monterey Bay, CA, remarkably few Bathochor- daeus charon (n = 15) were observed, while thousands of B. stygius have been encountered.”"]]
+[[!doi 10.1186/s41200-016-0075-9 desc="“In over two decades of exploring the mesopelagic waters of Monterey Bay, CA, remarkably few Bathochordaeus charon (n = 15) were observed, while thousands of B. stygius have been encountered.”"]]

Bus et avion
diff --git a/biblio/10.1186_s41200-016-0075-9.mdwn b/biblio/10.1186_s41200-016-0075-9.mdwn
new file mode 100644
index 0000000..a2e1fc1
--- /dev/null
+++ b/biblio/10.1186_s41200-016-0075-9.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="The first definitive record of the giant larvacean, Bathochordaeus charon, since its original description in 1900 and a range extension to the northeast Pacific Ocean"]]
+[[!tag Oikopleura]]
+
+R. E. Sherlock, K. R. Walz and B. H. Robison
+
+Marine Biodiversity Records 2016 9:79 doi:10.1186/s41200-016-0075-9
+
+The first definitive record of the giant larvacean, Bathochordaeus charon, since its original description in 1900 and a range extension to the northeast Pacific Ocean
+
+[[!doi 10.1186/s41200-016-0075-9 desc="“In over two decades of exploring the mesopelagic waters of Monterey Bay, CA, remarkably few Bathochor- daeus charon (n = 15) were observed, while thousands of B. stygius have been encountered.”"]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 3bee7dd..8b996e7 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -43,7 +43,8 @@ Phylogeny
    comparisons between strains of different oceans than within the Bergen gene
    pool.  We ignore whether and how Oikopleura dioica is subdivided into multiple
    species” ([[Denoeud et al., 2010|biblio/21097902]]).
-
+ - Giant Oikopleurid species, such as  exist in deeper waters. _Bathochordaeus charon_'s 18S
+   RNA is 97% identical to the one of _O. dioica_ ([[Sherlock and coll, 2016|biblio/10.1186_s41200-016-0075-9]]).
 
 Genome
 ------

À l'aéroport
diff --git a/biblio/10.1007_BF01986351.mdwn b/biblio/10.1007_BF01986351.mdwn
new file mode 100644
index 0000000..696be0b
--- /dev/null
+++ b/biblio/10.1007_BF01986351.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Architecture of, and water circulation and flow rate in, the house of the planktonic tunicate Oikopleura labradoriensis"]]
+[[!tag Oikopleura]]
+
+P. R. Flood
+
+Marine Biology October 1991, Volume 111, Issue 1, pp 95–111
+
+Architecture of, and water circulation and flow rate in, the house of the planktonic tunicate _Oikopleura labradoriensis_.
+
+[[!doi 10.1007/BF01986351 desc="Estimates that 35 ml of water are filtered each hour."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index bd8748d..3bee7dd 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -230,6 +230,8 @@ House
  - The food collecting filter in the house was described as a “self-cleaning
    filter” by [[Conley and coll., 2017|biblio/10.1002_lno.10680]], who observed
    its expansion and contraction at high spatial and temporal resolution.
+ - In _O. labradorensis_, [[Flood (1991)|biblio/10.1007_BF01986351]] estimated
+   that 35 ml of water were filtered per hour.
 
 
 Phenotypes

Dans le bus
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 1c4d8bd..bd8748d 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -178,6 +178,20 @@ Development
  - The tandem _propA_ and _propB_ genes control directly or indirectly _oik41a_
    and proper development of the house-secreting epithelium ([[Mikhaleva et
    al., 2018|biblio/30217597]]).
+ - The oral gland precursor is a syncytium with 4 nuclei that migrates
+   anteriorly.  The two differentiated oral gland cells have two nuclei each,
+   as demonstrated by a co-staining of nuclei (H2B-mCherry) and cell membrane
+   (PH-YF) by [[Kishi and coll, 2014|biblio/25224225]].
+ - The endodermal strand is the strand of 16 cells that lie in a single line.
+   The endodermal cells strand migrate from the tail to the trunk and give
+   rise to the posterior part of the digestive tract (rectum), but not the anus.
+   Removal of the trunk suggests that it is not necessary for initiation of the
+   migration ([[Kishi and coll, 2014|biblio/25224225]]).
+ - The subchordal cell precursors migrate along the right side of the notochord
+   in the space that has been filled with endodermal strand cells.  Amputation
+   experiments indicate that the posterior portion of the tail is required for
+   posterior migration of subchordal cell precursors ([[Kishi and coll,
+   2014|biblio/25224225]]).
 
 
 Anatomy

Dans le bus
diff --git a/biblio/25224225.mdwn b/biblio/25224225.mdwn
new file mode 100644
index 0000000..246984c
--- /dev/null
+++ b/biblio/25224225.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Long-distance cell migration during larval development in the appendicularian, Oikopleura dioica."]]
+[[!tag Oikopleura]]
+
+Dev Biol. 2014 Nov 15;395(2):299-306. doi:10.1016/j.ydbio.2014.09.006
+
+Kishi K, Onuma TA, Nishida H
+
+Long-distance cell migration during larval development in the appendicularian, Oikopleura dioica.
+
+[[!pmid 25224225 desc="The oral gland precursor is a syncytium with 4 nuclei that migrates anteriorly.  The two differentiated oral gland cells have two nuclei each, as demonstrated by a co-staining of nuclei (H2B-mCherry) and cell membrane (PH-YF).  The endodermal strand is the strand of 16 cells that lie in a single line.  The endodermal cells strand migrate from the tail to the trunk and give rise to the posterior part of the digestive tract (rectum), but not the anus.  Removal of the trunk suggests that it is not necessary for initiation of the migration.  The subchordal cell precursors migrate along the right side of the notochord in the space that has been filled with endodermal strand cells.  Amputation experiments indicate that the posterior portion of the tail is required for posterior migration of subchordal cell precursors."]]

correction
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 24e9ac7..1c4d8bd 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -214,7 +214,7 @@ House
    “the tails beat slowly when the suspended particles were numerous and rapidly
    when they were few”.
  - The food collecting filter in the house was described as a “self-cleaning
-   filter” by [[Conley and coll., 2017|biblio/10.1002_lno.1068]], who observed
+   filter” by [[Conley and coll., 2017|biblio/10.1002_lno.10680]], who observed
    its expansion and contraction at high spatial and temporal resolution.
 
 

Correction.
diff --git a/biblio/10.1111_j.1463-6395.1991.tb01203.x b/biblio/10.1111_j.1463-6395.1991.tb01203.x.mdwn
similarity index 94%
rename from biblio/10.1111_j.1463-6395.1991.tb01203.x
rename to biblio/10.1111_j.1463-6395.1991.tb01203.x.mdwn
index a9dfe6b..6163e7e 100644
--- a/biblio/10.1111_j.1463-6395.1991.tb01203.x
+++ b/biblio/10.1111_j.1463-6395.1991.tb01203.x.mdwn
@@ -1,4 +1,4 @@
-[[!meta title="The Subchordal Cells of Oikopleura dioica and O. albicans (Appendicularia, Chordata) "]]
+[[!meta title="The Subchordal Cells of Oikopleura dioica and O. albicans (Appendicularia, Chordata)"]]
 [[!tag Oikopleura]]
 
 Acta Zoologica Volume 72, Issue 4 December 1991, Pages 251-256

Café
diff --git a/biblio/10.1111_j.1463-6395.1991.tb01203.x b/biblio/10.1111_j.1463-6395.1991.tb01203.x
new file mode 100644
index 0000000..a9dfe6b
--- /dev/null
+++ b/biblio/10.1111_j.1463-6395.1991.tb01203.x
@@ -0,0 +1,10 @@
+[[!meta title="The Subchordal Cells of Oikopleura dioica and O. albicans (Appendicularia, Chordata) "]]
+[[!tag Oikopleura]]
+
+Acta Zoologica Volume 72, Issue 4 December 1991, Pages 251-256
+
+G. Fredriksson and R. Olsson
+
+The Subchordal Cells of _Oikopleura dioica_ and _O. albicans_ (Appendicularia, Chordata)
+
+[[!doi 10.1111/j.1463-6395.1991.tb01203.x desc="The subchordal cells display considerable variability of ultrastructure, which might be explained by the alternation between highly exocytic and highly endocytic stages.  O. dioica has two subchordal cells; O. albicans has “a multitude of quite small subchordal cells” with “essentially the same cytology”."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 94867c4..24e9ac7 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -188,7 +188,9 @@ Anatomy
    tight junctions ([[Nakashima and coll, 2011|biblio/21667277]]).
  - [[Onuma and coll., 2017|biblio/27837269]] published a comprehensive collection of
    surface electron microscopy pictures from external and internal organs.
-
+ - Two subchordal cells are found in the haemocel of the tail.  Utrastructure analysis
+   suggests the alternance of highly exocytic and highly endocytic states
+   ([[Fredriksson and Olsson, 1991|biblio/10.1111_j.1463-6395.1991.tb01203.x]]).
 
 Physiology
 ----------

Café
diff --git a/biblio/30356142.mdwn b/biblio/30356142.mdwn
new file mode 100644
index 0000000..5c1e6d4
--- /dev/null
+++ b/biblio/30356142.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Successful cryopreservation of coral larvae using vitrification and laser warming."]]
+[[!tag trehalose]]
+
+Daly J, Zuchowicz N, Nuñez Lendo CI, Khosla K, Lager C, Henley EM, Bischof J, Kleinhans FW, Lin C, Peters EC, Hagedorn M.
+
+Sci Rep. 2018 Oct 24;8(1):15714. doi:10.1038/s41598-018-34035-0
+
+Successful cryopreservation of coral larvae using vitrification and laser warming.
+
+[[!pmid 30356142 desc="Laser warming with gold nanorods.  Trehalose alone as a cryopresercant dehydrates the animals but does not permeate them but “3.5 M cryoprotectant solution (10% v/v propylene glycol, 5% v/v dimethyl sulfoxide, and 1 M trehalose in phosphate buffered saline)” does."]]

Café hier
diff --git a/biblio/30125266.mdwn b/biblio/30125266.mdwn
new file mode 100644
index 0000000..2b2305f
--- /dev/null
+++ b/biblio/30125266.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Variation graph toolkit improves read mapping by representing genetic variation in the reference."]]
+[[!tag alignment method]]
+
+Garrison E, Sirén J, Novak AM, Hickey G, Eizenga JM, Dawson ET, Jones W, Garg S, Markello C, Lin MF, Paten B, Durbin R.
+
+Nat Biotechnol. 2018 Oct;36(9):875-879. doi:10.1038/nbt.4227
+
+Variation graph toolkit improves read mapping by representing genetic variation in the reference.
+
+[[!pmid 30125266 desc="“Using the vg toolkit, we can construct or import a graph, modify it, visualize it, and use it as a reference.”"]]

Read just that part.
diff --git a/biblio/20333175.mdwn b/biblio/20333175.mdwn
new file mode 100644
index 0000000..b39e2c2
--- /dev/null
+++ b/biblio/20333175.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="On the origin and evolution of vertebrate olfactory receptor genes: comparative genome analysis among 23 chordate species."]]
+[[!tag Oikopleura olfaction]]
+
+Niimura Y.
+
+Genome Biol Evol. 2009 Apr 30;1:34-44. doi:10.1093/gbe/evp003
+
+On the origin and evolution of vertebrate olfactory receptor genes: comparative genome analysis among 23 chordate species.
+
+[[!pmid 20333175 desc="No ORs found in Oikopleura or Ciona"]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index ac14dfe..94867c4 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -121,7 +121,8 @@ Genes and pathways
  - Some muscle genes were duplicated in the _Oikopleura_ stem lineage
    ([[Inoue & Satoh|biblio/29319812]]), prehaps reflecting the importance of
    tail movements at larval and adult stages.
-
+ - No olfactory receptors have been found in _Oikopleura_ nor in _Ciona_
+   ([[Niimura, 2009|biblio/20333175]]).
 
 Transcriptome
 -------------

Plus de café.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 92d0022..ac14dfe 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -162,6 +162,8 @@ Development
    2018|biblio/29298334]]), and 132 ± 13 h (5.5 d) at 20 °C and 159 ± 20 h (6.9 d)
    at 15 °C ([[Troedsson and coll., 2002|biblio/10.3354_meps243083]]).  Note that
    culture conditions might differ.
+ - Increased food abundance increases egg number but does not change diameter nor
+   generation time ([[Troedsson and coll., 2002|biblio/10.3354_meps243083]]).
  - The _Oikopleura_ CNS possesses homologs of the vertebrate forebrain,
    hindbrain, and spinal cord, but not the midbrain.  No expression of
    _pax2/5/8_ is detected between the _otxa_ + _otxb_ and the _hox1_ territories.

Plus de café.
diff --git a/biblio/10.3354_meps243083.mdwn b/biblio/10.3354_meps243083.mdwn
new file mode 100644
index 0000000..75a252e
--- /dev/null
+++ b/biblio/10.3354_meps243083.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Resource allocation between somatic growth and reproductive output in the pelagic chordate Oikopleura dioica allows opportunistic response to nutritional variation."]]
+[[!tag Oikopleura]]
+
+Christofer Troedsson, Jean-Marie Bouquet, Dag L. Aksnes and Eric M. Thompson
+
+Mar Ecol Prog Ser Vol. 243: 83–91, 2002 Published November 13
+
+Resource allocation between somatic growth and reproductive output in the pelagic chordate _Oikopleura dioica_ allows opportunistic response to nutritional variation.
+
+[[!doi 10.3354/meps243083 desc="Defines four phases of development that transition with inflating the first house, growing the reproductive organ, and spawning. In phase 3, an increasing number of eggs is produced when available food increases, but the length of the phase and the size of the eggs stays comparable between standard and 1/6 food amounts.  Generation time was 132 ± 13 h (5.5 d) at 20 °C and 159 ± 20 h (6.9 d) at 15 °C."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index aa5c37e..92d0022 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -156,7 +156,12 @@ Development
 -----------
 
  - Generation times shortens when temperature rises.  First spawnings are seen
-   on day 6 at 14.2 °C, and on day 8 at 17.2 °C ([[Bouquet and coll., 2018|biblio/29298334]]).
+   on day 6 at 14.2 °C, and on day 8 at 17.2 °C ([[Bouquet and coll.,
+   2018|biblio/29298334]]).  Reported generation times in the litterature: 149 ± 2
+   h at 14.2 °C and 120 ± 6 h at 17.2 °C ([[Bouquet and coll.,
+   2018|biblio/29298334]]), and 132 ± 13 h (5.5 d) at 20 °C and 159 ± 20 h (6.9 d)
+   at 15 °C ([[Troedsson and coll., 2002|biblio/10.3354_meps243083]]).  Note that
+   culture conditions might differ.
  - The _Oikopleura_ CNS possesses homologs of the vertebrate forebrain,
    hindbrain, and spinal cord, but not the midbrain.  No expression of
    _pax2/5/8_ is detected between the _otxa_ + _otxb_ and the _hox1_ territories.

Café
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index f0c4244..aa5c37e 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -151,9 +151,12 @@ Tools
  - DNAi was used to screen for maternal genes ([[Omotezako et al., 2017|biblio/28281645]]).
  - CRISPR-Cas9 was used to induce genomic microdeletions ([[Deng, Henriet and Chourrout, 2018|biblio/30293719]]).
 
+
 Development
 -----------
 
+ - Generation times shortens when temperature rises.  First spawnings are seen
+   on day 6 at 14.2 °C, and on day 8 at 17.2 °C ([[Bouquet and coll., 2018|biblio/29298334]]).
  - The _Oikopleura_ CNS possesses homologs of the vertebrate forebrain,
    hindbrain, and spinal cord, but not the midbrain.  No expression of
    _pax2/5/8_ is detected between the _otxa_ + _otxb_ and the _hox1_ territories.
@@ -178,6 +181,7 @@ Anatomy
  - [[Onuma and coll., 2017|biblio/27837269]] published a comprehensive collection of
    surface electron microscopy pictures from external and internal organs.
 
+
 Physiology
 ----------
 
@@ -203,6 +207,7 @@ House
    filter” by [[Conley and coll., 2017|biblio/10.1002_lno.1068]], who observed
    its expansion and contraction at high spatial and temporal resolution.
 
+
 Phenotypes
 ----------
 

Café
diff --git a/biblio/27837269.mdwn b/biblio/27837269.mdwn
new file mode 100644
index 0000000..566a7bf
--- /dev/null
+++ b/biblio/27837269.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Internal and external morphology of adults of the appendicularian, Oikopleura dioica: an SEM study."]]
+[[!tag Oikopleura]]
+
+Onuma TA, Isobe M, Nishida H.
+
+Cell Tissue Res. 2017 Feb;367(2):213-227. doi:10.1007/s00441-016-2524-5
+
+Internal and external morphology of adults of the appendicularian, Oikopleura dioica: an SEM study.
+
+[[!pmid 27837269 desc="A good reminder that there are also a few neural and subchordal cells in the tail."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 5860ca9..f0c4244 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -175,7 +175,8 @@ Anatomy
  - The adult tail is made of three layers of cells: epithelium, muscle, notochord.
    The fins of the tail are made from single epithelial cells joining each side with
    tight junctions ([[Nakashima and coll, 2011|biblio/21667277]]).
-
+ - [[Onuma and coll., 2017|biblio/27837269]] published a comprehensive collection of
+   surface electron microscopy pictures from external and internal organs.
 
 Physiology
 ----------

Old
diff --git a/biblio/29237070.mdwn b/biblio/29237070.mdwn
new file mode 100644
index 0000000..8b29fc0
--- /dev/null
+++ b/biblio/29237070.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="Rolling circle amplification shows a sinusoidal template length-dependent amplification bias."]]
+[[!tag amplification method]]
+
+Nucleic Acids Res. 2018 Jan 25;46(2):538-545. doi:10.1093/nar/gkx1238
+
+Joffroy B, Uca YO, Prešern D, Doye JPK, Schmidt TL.
+
+Rolling circle amplification shows a sinusoidal template length-dependent amplification bias.
+
+[[!pmid 29237070 desc="Small circles of length ~70-75, 80-85, ... are very hard to amplify with
+Phi29, and to a lesser extent with Bst 2.0"]]

Café
diff --git a/biblio/21667277.mdwn b/biblio/21667277.mdwn
new file mode 100644
index 0000000..38c0f23
--- /dev/null
+++ b/biblio/21667277.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Forming a tough shell via an intracellular matrix and cellular junctions in the tail epidermis of Oikopleura dioica (Chordata: Tunicata: Appendicularia)."]]
+[[!tag Oikopleura]]
+
+Nakashima K, Nishino A, Hirose E.
+
+Naturwissenschaften. 2011 Aug;98(8):661-9. doi:10.1007/s00114-011-0815-y
+
+Forming a tough shell via an intracellular matrix and cellular junctions in the tail epidermis of Oikopleura dioica (Chordata: Tunicata: Appendicularia).
+
+[[!pmid 21667277 desc="In larvae, the tail is enclosed in the larval enveloppe.  In adults, the tail fins are made of single triangular epithelial cells attached with tight junctions to the other epitheilal cells covering the tail on the left and right sides.  The muscle cells are attached to the epithelial cells via a basal membrane.  Up and down the notochord, a sinus separates the muscle cells of each side.  In the muscle cells, mitochondria are packed on the exterior side and muscle fibers are on the interior side."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index ae9a749..5860ca9 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -169,6 +169,14 @@ Development
    al., 2018|biblio/30217597]]).
 
 
+Anatomy
+-------
+
+ - The adult tail is made of three layers of cells: epithelium, muscle, notochord.
+   The fins of the tail are made from single epithelial cells joining each side with
+   tight junctions ([[Nakashima and coll, 2011|biblio/21667277]]).
+
+
 Physiology
 ----------
 

syntax again
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 6610cb0..ae9a749 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -24,7 +24,7 @@ Some links:
 
 Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
 µm in size), _Tetraselmis suecica_ (9.5 µm), and the chlorophyte _Chlorella
-sp._ (3.5 µm) [[Acuña and Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608.mdwn]].
+sp._ (3.5 µm) [[Acuña and Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608]].
 
 Parasites: _Oodinium pouchetii_ and others.
 

syntax
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index aaa260e..6610cb0 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -191,7 +191,7 @@ House
    “the tails beat slowly when the suspended particles were numerous and rapidly
    when they were few”.
  - The food collecting filter in the house was described as a “self-cleaning
-   filter” by [[Conley and coll., 2017biblio|10.1002_lno.1068]], who observed
+   filter” by [[Conley and coll., 2017|biblio/10.1002_lno.1068]], who observed
    its expansion and contraction at high spatial and temporal resolution.
 
 Phenotypes

Café
diff --git a/biblio/10.1002_lno.10680.mdwn b/biblio/10.1002_lno.10680.mdwn
new file mode 100644
index 0000000..b648109
--- /dev/null
+++ b/biblio/10.1002_lno.10680.mdwn
@@ -0,0 +1,14 @@
+[[!meta title="A self‐cleaning biological filter: How appendicularians mechanically control particle adhesion and removal."]]
+[[!tag Oikopleura]]
+
+Keats R. Conley
+Brad J. Gemmell
+Jean‐Marie Bouquet
+Eric M. Thompson and
+Kelly R. Sutherland
+
+Limnol. Oceanogr. 63, 2018, 927–938
+
+A self‐cleaning biological filter: How appendicularians mechanically control particle adhesion and removal.
+
+[[!doi 10.1002/lno.10680 desc="Smaller particles adhere more than larger ones."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index dc59478..aaa260e 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -190,6 +190,9 @@ House
    1986|biblio/10.1007_BF00312043]].  He also observed that, when in the house,
    “the tails beat slowly when the suspended particles were numerous and rapidly
    when they were few”.
+ - The food collecting filter in the house was described as a “self-cleaning
+   filter” by [[Conley and coll., 2017biblio|10.1002_lno.1068]], who observed
+   its expansion and contraction at high spatial and temporal resolution.
 
 Phenotypes
 ----------

In the house, ...
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index d8e90b8..dc59478 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -183,6 +183,14 @@ Physiology
    digestion or chemical defence ([[Martí-Solans et al., 2016|biblio/27406791]]).
 
 
+House
+-----
+
+ - The house was observed in details with India ink coloration by [[Fenaux
+   1986|biblio/10.1007_BF00312043]].  He also observed that, when in the house,
+   “the tails beat slowly when the suspended particles were numerous and rapidly
+   when they were few”.
+
 Phenotypes
 ----------
 

Café
diff --git a/biblio/10.1007_BF00312043.mdwn b/biblio/10.1007_BF00312043.mdwn
new file mode 100644
index 0000000..f94dce3
--- /dev/null
+++ b/biblio/10.1007_BF00312043.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="The house of Oikopleura dioica (Tunicata, Appendicularia): Structure and functions"]]
+[[!tag Oikopleura]]
+
+Fenaux R
+
+Zoomorphology October 1986, Volume 106, Issue 4, pp 224–231
+
+The house of Oikopleura dioica (Tunicata, Appendicularia): Structure and functions
+
+[[!doi 10.1007/BF00312043 desc="“In our cultures, the tails beat slowly when the suspended particles were numerous and rapidly when they were few.”"]]

Typo
diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index e71ffe3..b5bfe73 100644
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -33,6 +33,6 @@ Methods for enriching capped RNAs.
 Atypical caps (work in progress).
 
  - 5′-phospho-ADP-ribosylated RNA/DNA cap, synthethysed by RNA
-   2′-phosphotransferase Tpt1 ([[Munir, Banerjee and Shuman, 2018|biblio/3020286]]).
+   2′-phosphotransferase Tpt1 ([[Munir, Banerjee and Shuman, 2018|biblio/30202863]]).
 
 [[!inline pages="tagged(cap)" limit=0]]

Cap
diff --git a/biblio/30202863.mdwn b/biblio/30202863.mdwn
new file mode 100644
index 0000000..cc7fe65
--- /dev/null
+++ b/biblio/30202863.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="NAD+-dependent synthesis of a 5'-phospho-ADP-ribosylated RNA/DNA cap by RNA 2'-phosphotransferase Tpt1."]]
+[[!tag cap]]
+
+Nucleic Acids Res. 2018 Oct 12;46(18):9617-9624. doi:10.1093/nar/gky792
+
+Munir A, Banerjee A, Shuman S.
+
+NAD+-dependent synthesis of a 5'-phospho-ADP-ribosylated RNA/DNA cap by RNA 2'-phosphotransferase Tpt1.
+
+[[!pmid 30202863 desc="Tpt1 from Aeropyrum pernix (ApeTpt1)."]]
diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index 082ef11..e71ffe3 100644
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -30,5 +30,9 @@ Methods for enriching capped RNAs.
  - [[Kwak et al., 2013|biblio/23430654]] modified oligo-capping to create Pro-cap, a method for nuclear
    run-on analysis at single-nucleotide resulution.
 
+Atypical caps (work in progress).
+
+ - 5′-phospho-ADP-ribosylated RNA/DNA cap, synthethysed by RNA
+   2′-phosphotransferase Tpt1 ([[Munir, Banerjee and Shuman, 2018|biblio/3020286]]).
 
 [[!inline pages="tagged(cap)" limit=0]]

tag
diff --git a/biblio/30293719.mdwn b/biblio/30293719.mdwn
index 0038211..badc3bd 100644
--- a/biblio/30293719.mdwn
+++ b/biblio/30293719.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Prevalence of Mutation-Prone Microhomology-Mediated End Joining in a Chordate Lacking the c-NHEJ DNA Repair Pathway."]]
-[[!tag Oikopleura mutation]]
+[[!tag Oikopleura mutation CRISPR]]
 
 Deng W, Henriet S, Chourrout D.
 

Café
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 30589bb..d8e90b8 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -65,8 +65,10 @@ Genome
    2010|biblio/21097902]])
  - Chromatin domains are rarely longer than 7 nucleosomes ([[Navratilova et al., 2017|biblio/28115992]]).
  - Some 5-methylcytosine was detected by MeDIP-chip by ([[Navratilova et al., 2017|biblio/28115992]]).
- - The entire machinery required for performing NHEJ repair of DSB (which is
+ - The entire machinery required for performing classical NHEJ repair of DSB (which is
    conserved from yeast to mammals) is undetectable ([[Denoeud et al., 2010|biblio/21097902]]).
+ - An alternative or microhomology (MH)-driven end joining pathway is active
+   and triggers microdeletions at the joining site ([[Deng, Henriet and Chourrout, 2018|biblio/30293719]]).
  - Only a partial mitochondrial genome was reconstituted in [[Denoeud et al.,
    2010|biblio/21097902]], due to cloning and sequencing difficulties that may
    have been caused by oligo-dT stretches.  A/T-rich codons are more frequent than
@@ -147,7 +149,7 @@ Tools
 -----
 
  - DNAi was used to screen for maternal genes ([[Omotezako et al., 2017|biblio/28281645]]).
-
+ - CRISPR-Cas9 was used to induce genomic microdeletions ([[Deng, Henriet and Chourrout, 2018|biblio/30293719]]).
 
 Development
 -----------

Café
diff --git a/biblio/30293719.mdwn b/biblio/30293719.mdwn
new file mode 100644
index 0000000..0038211
--- /dev/null
+++ b/biblio/30293719.mdwn
@@ -0,0 +1,18 @@
+[[!meta title="Prevalence of Mutation-Prone Microhomology-Mediated End Joining in a Chordate Lacking the c-NHEJ DNA Repair Pathway."]]
+[[!tag Oikopleura mutation]]
+
+Deng W, Henriet S, Chourrout D.
+
+Curr Biol. 2018 Sep 27. pii: S0960-9822(18)31132-1. doi:10.1016/j.cub.2018.08.048
+
+Prevalence of Mutation-Prone Microhomology-Mediated End Joining in a Chordate Lacking the c-NHEJ DNA Repair Pathway.
+
+[[!pmid 30293719 desc="In circularisation assays, the ratio between end-joining
+and homologous recombination is similar in O. dioica compared with other
+eukaryotes.  Nevertheless, end-joining is more likely to concatenate than to
+circularise linear plasmids.  The presence of microhomologies trigger
+microdeletions, consistend with the absence of the “classical” end-joining
+pathway.  These microdeletions are much more frequent than in other organisms.
+Comparisons between Norvegian and Japanese strains suggest that the
+microdeletions could be part of the mechansism driving the compaction of O.
+dioica's genome."]]

Masted 2018
diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index dc83bbe..55eb466 100644
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -20,6 +20,7 @@ Different kinds of surfactants:
  - ABIL WE09 (polysiloxane–polycetyl–polyethylene glycol copolymer; primary paper: [[Dielh _et al._, 2004|biblio/14990785]])
  - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]])
  - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]
+ - 3% fluorosurfactant (RAN Biotechnologies) in Novec HFE-7500 / 0.015% Tween 80 for double emulsions in [[Masted and coll., 2018|biblio/10.1101_409086]].
 
 Reactive droplets:
 

Syntaxx
diff --git a/biblio/10.1101_409086.mdwn b/biblio/10.1101_409086.mdwn
index afb8cfe..fa04afd 100644
--- a/biblio/10.1101_409086.mdwn
+++ b/biblio/10.1101_409086.mdwn
@@ -7,4 +7,4 @@ bioRxiv, September 6, 2018.
 
 Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting
 
-[[!DOI: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing.  When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target.  Emulsions were prepared in Dolomite chips."]]
+[[!doi 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing.  When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target.  Emulsions were prepared in Dolomite chips."]]

syntax
diff --git a/biblio/10.1101_409086.mdwn b/biblio/10.1101_409086.mdwn
index c4715df..afb8cfe 100644
--- a/biblio/10.1101_409086.mdwn
+++ b/biblio/10.1101_409086.mdwn
@@ -7,4 +7,4 @@ bioRxiv, September 6, 2018.
 
 Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting
 
-[[!doi: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing.  When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target.  Emulsions were prepared in Dolomite chips."]]
+[[!DOI: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing.  When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target.  Emulsions were prepared in Dolomite chips."]]

bioRxiv
diff --git a/biblio/10.1101_409086.mdwn b/biblio/10.1101_409086.mdwn
new file mode 100644
index 0000000..c4715df
--- /dev/null
+++ b/biblio/10.1101_409086.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting"]]
+[[!tag emulsion Nanopore HPV]]
+
+Esben Bjoern Madsen, Thomas Kvist, Ida Hoijer, Adam Ameur, Marie Just Mikkelsen
+
+bioRxiv, September 6, 2018.
+
+Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting
+
+[[!doi: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing.  When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target.  Emulsions were prepared in Dolomite chips."]]

A day in the life of an Oikopleura lab.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 88c6055..30589bb 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -20,6 +20,7 @@ Some links:
  - OikoBase: <http://oikoarrays.biology.uiowa.edu/Oiko/> ([[Danks et al., 2013|biblio/23185044]])
  - Études sur les Appendiculaires du Détroit de Messine, Hermann Fol, 1872
    ([[ark:/13960/t7fr0vj77|https://archive.org/details/cbarchive_100233_etudessurlesappendiculairesdud1821]]).
+ - [A day in the life of an Oikopleura lab](http://thenode.biologists.com/day-life-oikopleura-lab/).
 
 Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
 µm in size), _Tetraselmis suecica_ (9.5 µm), and the chlorophyte _Chlorella

Café.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 964e294..88c6055 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -25,6 +25,8 @@ Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
 µm in size), _Tetraselmis suecica_ (9.5 µm), and the chlorophyte _Chlorella
 sp._ (3.5 µm) [[Acuña and Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608.mdwn]].
 
+Parasites: _Oodinium pouchetii_ and others.
+
 
 Phylogeny
 ---------

Café
diff --git a/biblio/30228204.mdwn b/biblio/30228204.mdwn
new file mode 100644
index 0000000..079e2f1
--- /dev/null
+++ b/biblio/30228204.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics."]]
+[[!tag single_cell Ciona_intestinalis]]
+
+Genes Dev. 2018 Sep 18. doi:10.1101/gad.317669.118
+
+Horie T, Horie R, Chen K, Cao C, Nakagawa M, Kusakabe TG, Satoh N, Sasakura Y, Levine M.
+
+Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics.
+
+[[!pmid 30228204 desc="Ptf1a and Meis can reprogram the CNS of Ciona intestinalis into a dopaminergic cell type."]]

Correct links.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 88e4c89..964e294 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -23,7 +23,7 @@ Some links:
 
 Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
 µm in size), _Tetraselmis suecica_ (9.5 µm), and the chlorophyte _Chlorella
-sp._ (3.5 µm) [[Acuña and Kiefer, 2000|10.4319_lo.2000.45.3.0608.mdwn]].
+sp._ (3.5 µm) [[Acuña and Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608.mdwn]].
 
 
 Phylogeny
@@ -204,6 +204,6 @@ Ecology
    database of DNA barcodes.
  - Carbon output of _O. dioica_ is either their house or fecal pellets, the
    ratio of which may depend on food concentration ([[Acuña and
-   Kiefer, 2000|10.4319_lo.2000.45.3.0608.mdwn]]).
+   Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608.mdwn]]).
 
 [[!inline pages="tagged(Oikopleura)" actions="no" limit=0]]

Food
diff --git a/biblio/10.4319_lo.2000.45.3.0608.mdwn b/biblio/10.4319_lo.2000.45.3.0608.mdwn
new file mode 100644
index 0000000..7d08111
--- /dev/null
+++ b/biblio/10.4319_lo.2000.45.3.0608.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Functional response of the appendicularian Oikopleura dioica."]]
+[[!tag Oikopleura]]
+
+José Luis Acuña and Markus Kiefer.
+
+Limnology and Oceanography Volume 45, Issue 3, Pages 608–18, 26 April 2000
+
+Functional response of the appendicularian Oikopleura dioica.
+
+[[!doi 10.4319/lo.2000.45.3.0608 desc="“Particles cleared from suspension by O. dioica are ingested and transformed into fast‐sinking fecal pellets at low FC, while they are mainly accumulated into slow to fast‐sinking filter houses at high FC”.  Food used in this study: Isochrysis galbana (5.5 µm in size), Tetraselmis suecica (9.5 µm), and Chlorella sp. (3.5 µm)."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 80701f1..88e4c89 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -21,6 +21,11 @@ Some links:
  - Études sur les Appendiculaires du Détroit de Messine, Hermann Fol, 1872
    ([[ark:/13960/t7fr0vj77|https://archive.org/details/cbarchive_100233_etudessurlesappendiculairesdud1821]]).
 
+Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
+µm in size), _Tetraselmis suecica_ (9.5 µm), and the chlorophyte _Chlorella
+sp._ (3.5 µm) [[Acuña and Kiefer, 2000|10.4319_lo.2000.45.3.0608.mdwn]].
+
+
 Phylogeny
 ---------
 
@@ -197,5 +202,8 @@ Ecology
  - It was already reported to be frequent in Japanese waters [[in 1907 by T. Aida|biblio/AA0069577]].
  - As of August 2018, _O. dioica_ is not yet found in the [BOLD](http://www.boldsystems.org/)
    database of DNA barcodes.
+ - Carbon output of _O. dioica_ is either their house or fecal pellets, the
+   ratio of which may depend on food concentration ([[Acuña and
+   Kiefer, 2000|10.4319_lo.2000.45.3.0608.mdwn]]).
 
 [[!inline pages="tagged(Oikopleura)" actions="no" limit=0]]

Cafés
diff --git a/biblio/10.1093_plankt_2.2.145.mdwn b/biblio/10.1093_plankt_2.2.145.mdwn
new file mode 100644
index 0000000..94b205c
--- /dev/null
+++ b/biblio/10.1093_plankt_2.2.145.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Répartition des Appendiculaires au large des côtes de Californie"]]
+[[!tag Oikopleura]]
+
+Journal of Plankton Research, Volume 2, Issue 2, 1 April 1980, Pages 145–167 doi:10.1093/plankt/2.2.145
+
+R. Fenaux S. Dallot
+
+Répartition des Appendiculaires au large des côtes de Californie 
+
+[[!doi 10.1093/plankt/2.2.145 desc="Distributions of appendicularian species can be sorted according to the presence or absence of O. dioica (other species tend to co-occur by clusters).  O. dioica tends to co-occur with F. Tenella.  O. longicauda is the most abundant.  The total amount of appendicularians is higher where O. dioica is found."]]

Typo
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
index 2b8c1da..e847426 100644
--- "a/Debian/debi\303\242neries/okinawa.en.po"
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2018-09-23 12:51+0000\n"
-"PO-Revision-Date: 2018-09-23 21:51+0900\n"
+"PO-Revision-Date: 2018-09-24 07:37+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -78,7 +78,7 @@ msgid ""
 "restaurants#grano)).  Si vous venez le visiter, faites-moi signe !"
 msgstr ""
 "On the work side, I joined the Okinawa Institute of Science and Technology "
-"Graduate University (OIST).  It is a formidable place, [open to the public])"
+"Graduate University (OIST).  It is a formidable place, [open to the public]"
 "(https://www.oist.jp/unguided-campus-visit) even on week-end (note the "
 "opening hours of the [café](https://www.oist.jp/visitors-center-shops-"
 "restaurants#grano)).  If you come visit, please let me know!"

)
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
index 0195240..2b8c1da 100644
--- "a/Debian/debi\303\242neries/okinawa.en.po"
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2018-09-23 12:51+0000\n"
-"PO-Revision-Date: 2018-09-23 21:48+0900\n"
+"PO-Revision-Date: 2018-09-23 21:51+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -37,14 +37,6 @@ msgid "[[!meta title=\"J'ai déménagé à Okinawa !\"]]\n"
 msgstr "[[!meta title=\"I moved to Okinawa!\"]]\n"
 
 #. type: Plain text
-#, fuzzy
-#| msgid ""
-#| "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
-#| "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
-#| "[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
-#| "traditionnelles faisant usage d'un grand nombre de tambours, ayant "
-#| "souvent lieu vers la fin de mois d'août.  Chaque quartier a sa propre "
-#| "troupe et nous espérons pouvoir nous y joindre pour l'année prochaine."
 msgid ""
 "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
 "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
@@ -55,7 +47,7 @@ msgid ""
 msgstr ""
 "I moved with my family to Okinawa in August, in the Akano neighborhood in "
 "the Uruma city.  We arrived on time to see a bunch of [eisaa](https://en."
-"wikipedia.org/wiki/Eisa_(dance)), traditional dances using lots for drums, "
+"wikipedia.org/wiki/Eisa_(dance%29), traditional dances using lots for drums, "
 "that often take place at the end of August.  Each neighborhood has its own "
 "band and we hope we can join next year."
 

updated PO files
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
index 2fd4912..0195240 100644
--- "a/Debian/debi\303\242neries/okinawa.en.po"
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -6,7 +6,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2018-09-23 12:46+0000\n"
+"POT-Creation-Date: 2018-09-23 12:51+0000\n"
 "PO-Revision-Date: 2018-09-23 21:48+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
@@ -37,10 +37,18 @@ msgid "[[!meta title=\"J'ai déménagé à Okinawa !\"]]\n"
 msgstr "[[!meta title=\"I moved to Okinawa!\"]]\n"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
+#| "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
+#| "[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
+#| "traditionnelles faisant usage d'un grand nombre de tambours, ayant "
+#| "souvent lieu vers la fin de mois d'août.  Chaque quartier a sa propre "
+#| "troupe et nous espérons pouvoir nous y joindre pour l'année prochaine."
 msgid ""
 "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
 "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
-"[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
+"[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance%29), des danses "
 "traditionnelles faisant usage d'un grand nombre de tambours, ayant souvent "
 "lieu vers la fin de mois d'août.  Chaque quartier a sa propre troupe et nous "
 "espérons pouvoir nous y joindre pour l'année prochaine."

)
diff --git "a/Debian/debi\303\242neries/okinawa.mdwn" "b/Debian/debi\303\242neries/okinawa.mdwn"
index bb9ef08..3ad57ca 100644
--- "a/Debian/debi\303\242neries/okinawa.mdwn"
+++ "b/Debian/debi\303\242neries/okinawa.mdwn"
@@ -6,7 +6,7 @@
 
 J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier d'Akano,
 ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs
-[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses traditionnelles
+[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance%29), des danses traditionnelles
 faisant usage d'un grand nombre de tambours, ayant souvent lieu vers la fin de
 mois d'août.  Chaque quartier a sa propre troupe et nous espérons pouvoir nous
 y joindre pour l'année prochaine.

!!
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
index 071659e..2fd4912 100644
--- "a/Debian/debi\303\242neries/okinawa.en.po"
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2018-09-23 12:46+0000\n"
-"PO-Revision-Date: 2018-09-23 21:46+0900\n"
+"PO-Revision-Date: 2018-09-23 21:48+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -34,7 +34,7 @@ msgstr "[[!tag Debian]]\n"
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta title=\"J'ai déménagé à Okinawa !\"]]\n"
-msgstr "[[!meta title=\"I moved to Okinawa!!\"]]\n"
+msgstr "[[!meta title=\"I moved to Okinawa!\"]]\n"
 
 #. type: Plain text
 msgid ""

Yasasa.
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
index 46931ce..071659e 100644
--- "a/Debian/debi\303\242neries/okinawa.en.po"
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2018-09-23 12:46+0000\n"
-"PO-Revision-Date: 2018-09-23 21:45+0900\n"
+"PO-Revision-Date: 2018-09-23 21:46+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -37,14 +37,6 @@ msgid "[[!meta title=\"J'ai déménagé à Okinawa !\"]]\n"
 msgstr "[[!meta title=\"I moved to Okinawa!!\"]]\n"
 
 #. type: Plain text
-#, fuzzy
-#| msgid ""
-#| "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
-#| "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
-#| "[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
-#| "traditionelles faisant usage d'un grand nombre de tambours, ayant souvent "
-#| "lieu vers la fin de mois d'août.  Chaque quartier a sa propre troupe et "
-#| "nous espérons pouvoir nous y joindre pour l'année prochaine."
 msgid ""
 "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
 "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
@@ -60,16 +52,6 @@ msgstr ""
 "band and we hope we can join next year."
 
 #. type: Plain text
-#, fuzzy
-#| msgid ""
-#| "Nous habitons dans un immeuble avec une connection en fibre optique "
-#| "partagée.  Bon ping vers la métropole, mais le débit pour les gros "
-#| "téléchargements est catastrophique le soir quand toutes les familles sont "
-#| "connectées en même temps.  Impossible de réussir un simple `sbuild-update "
-#| "-dragu unstable`, alors je n'ai malheureusement pas réussi à contribuer "
-#| "qui que ce soit à Debian.  C'est frustrant mais il y a peut-être des "
-#| "solutions à chercher du côté de notre [forge GitLab](https://salsa.debian."
-#| "org)."
 msgid ""
 "Nous habitons dans un immeuble avec une connexion en fibre optique "
 "partagée.  Bon ping vers la métropole, mais le débit pour les gros "
@@ -81,21 +63,13 @@ msgid ""
 msgstr ""
 "We live in a concrete building with a shared optic fiber connection.  It has "
 "a good ping to the mainland, but the speed for big downloads is catastrophic "
-"in the evenings, when all families are using the fibre at the same time.  "
+"in the evenings, when all families are using the fiber at the same time.  "
 "Impossible to manage a simple `sbuild-update -dragu unstable`, and I could "
 "not contribute anything to Debian since them.  It is frustrating; however "
 "there might be solutions through our [GitLab forge](https://salsa.debian."
 "org)."
 
 #. type: Plain text
-#, fuzzy
-#| msgid ""
-#| "Côté travail, j'ai rejoint l'université Okinawa Institue of Science and "
-#| "Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, "
-#| "[ouvert au public](https://www.oist.jp/unguided-campus-visit) même le "
-#| "week-end (notez les horaires du [café](https://www.oist.jp/visitors-"
-#| "center-shops-restaurants#grano)).  Si vous venez le visiter, faites-moi "
-#| "signe !"
 msgid ""
 "Côté travail, j'ai rejoint l'université Okinawa Institute of Science and "
 "Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, "

updated PO files
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
index 6f35f48..46931ce 100644
--- "a/Debian/debi\303\242neries/okinawa.en.po"
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -3,66 +3,108 @@
 # This file is distributed under the same license as the PACKAGE package.
 # FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
 #
-#, fuzzy
 msgid ""
 msgstr ""
-"Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2018-09-23 12:34+0000\n"
-"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
-"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
-"Language-Team: LANGUAGE <LL@li.org>\n"
-"Language: \n"
+"Project-Id-Version: \n"
+"POT-Creation-Date: 2018-09-23 12:46+0000\n"
+"PO-Revision-Date: 2018-09-23 21:45+0900\n"
+"Last-Translator: Charles Plessy <toto@example.com>\n"
+"Language-Team: \n"
+"Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
+"X-Generator: Poedit 1.8.11\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta date=\"Sun, 23 Sep 2018 21:33:11 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta date=\"Sun, 23 Sep 2018 21:33:11 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta updated=\"Sun, 23 Sep 2018 21:33:11 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta updated=\"Sun, 23 Sep 2018 21:33:11 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!tag Debian]]\n"
-msgstr ""
+msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta title=\"J'ai déménagé à Okinawa !\"]]\n"
-msgstr ""
+msgstr "[[!meta title=\"I moved to Okinawa!!\"]]\n"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
+#| "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
+#| "[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
+#| "traditionelles faisant usage d'un grand nombre de tambours, ayant souvent "
+#| "lieu vers la fin de mois d'août.  Chaque quartier a sa propre troupe et "
+#| "nous espérons pouvoir nous y joindre pour l'année prochaine."
 msgid ""
 "J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
 "d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
 "[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
-"traditionelles faisant usage d'un grand nombre de tambours, ayant souvent "
+"traditionnelles faisant usage d'un grand nombre de tambours, ayant souvent "
 "lieu vers la fin de mois d'août.  Chaque quartier a sa propre troupe et nous "
 "espérons pouvoir nous y joindre pour l'année prochaine."
 msgstr ""
+"I moved with my family to Okinawa in August, in the Akano neighborhood in "
+"the Uruma city.  We arrived on time to see a bunch of [eisaa](https://en."
+"wikipedia.org/wiki/Eisa_(dance)), traditional dances using lots for drums, "
+"that often take place at the end of August.  Each neighborhood has its own "
+"band and we hope we can join next year."
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "Nous habitons dans un immeuble avec une connection en fibre optique "
+#| "partagée.  Bon ping vers la métropole, mais le débit pour les gros "
+#| "téléchargements est catastrophique le soir quand toutes les familles sont "
+#| "connectées en même temps.  Impossible de réussir un simple `sbuild-update "
+#| "-dragu unstable`, alors je n'ai malheureusement pas réussi à contribuer "
+#| "qui que ce soit à Debian.  C'est frustrant mais il y a peut-être des "
+#| "solutions à chercher du côté de notre [forge GitLab](https://salsa.debian."
+#| "org)."
 msgid ""
-"Nous habitons dans un immeuble avec une connection en fibre optique "
+"Nous habitons dans un immeuble avec une connexion en fibre optique "
 "partagée.  Bon ping vers la métropole, mais le débit pour les gros "
 "téléchargements est catastrophique le soir quand toutes les familles sont "
-"connectées en même temps.  Impossible de réussir un simple `sbuild-update "
-"-dragu unstable`, alors je n'ai malheureusement pas réussi à contribuer qui "
+"connectées en même temps.  Impossible de réussir un simple `sbuild-update -"
+"dragu unstable`, alors je n'ai malheureusement pas réussi à contribuer qui "
 "que ce soit à Debian.  C'est frustrant mais il y a peut-être des solutions à "
 "chercher du côté de notre [forge GitLab](https://salsa.debian.org)."
 msgstr ""
+"We live in a concrete building with a shared optic fiber connection.  It has "
+"a good ping to the mainland, but the speed for big downloads is catastrophic "
+"in the evenings, when all families are using the fibre at the same time.  "
+"Impossible to manage a simple `sbuild-update -dragu unstable`, and I could "
+"not contribute anything to Debian since them.  It is frustrating; however "
+"there might be solutions through our [GitLab forge](https://salsa.debian."
+"org)."
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "Côté travail, j'ai rejoint l'université Okinawa Institue of Science and "
+#| "Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, "
+#| "[ouvert au public](https://www.oist.jp/unguided-campus-visit) même le "
+#| "week-end (notez les horaires du [café](https://www.oist.jp/visitors-"
+#| "center-shops-restaurants#grano)).  Si vous venez le visiter, faites-moi "
+#| "signe !"
 msgid ""
-"Côté travail, j'ai rejoint l'université Okinawa Institue of Science and "
+"Côté travail, j'ai rejoint l'université Okinawa Institute of Science and "
 "Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, "
-"[ouvert au public](https://www.oist.jp/unguided-campus-visit) même le "
-"week-end (notez les horaires du "
-"[café](https://www.oist.jp/visitors-center-shops-restaurants#grano)).  Si "
-"vous venez le visiter, faites-moi signe !"
+"[ouvert au public](https://www.oist.jp/unguided-campus-visit) même le week-"
+"end (notez les horaires du [café](https://www.oist.jp/visitors-center-shops-"
+"restaurants#grano)).  Si vous venez le visiter, faites-moi signe !"
 msgstr ""
+"On the work side, I joined the Okinawa Institute of Science and Technology "
+"Graduate University (OIST).  It is a formidable place, [open to the public])"
+"(https://www.oist.jp/unguided-campus-visit) even on week-end (note the "
+"opening hours of the [café](https://www.oist.jp/visitors-center-shops-"
+"restaurants#grano)).  If you come visit, please let me know!"

yasasa
diff --git "a/Debian/debi\303\242neries/okinawa.mdwn" "b/Debian/debi\303\242neries/okinawa.mdwn"
index b8aaaa7..bb9ef08 100644
--- "a/Debian/debi\303\242neries/okinawa.mdwn"
+++ "b/Debian/debi\303\242neries/okinawa.mdwn"
@@ -6,12 +6,12 @@
 
 J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier d'Akano,
 ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs
-[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses traditionelles
+[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses traditionnelles
 faisant usage d'un grand nombre de tambours, ayant souvent lieu vers la fin de
 mois d'août.  Chaque quartier a sa propre troupe et nous espérons pouvoir nous
 y joindre pour l'année prochaine.
 
-Nous habitons dans un immeuble avec une connection en fibre optique partagée.
+Nous habitons dans un immeuble avec une connexion en fibre optique partagée.
 Bon ping vers la métropole, mais le débit pour les gros téléchargements est
 catastrophique le soir quand toutes les familles sont connectées en même temps.
 Impossible de réussir un simple `sbuild-update -dragu unstable`, alors je n'ai
@@ -19,7 +19,7 @@ malheureusement pas réussi à contribuer qui que ce soit à Debian.  C'est
 frustrant mais il y a peut-être des solutions à chercher du côté de notre
 [forge GitLab](https://salsa.debian.org).
 
-Côté travail, j'ai rejoint l'université Okinawa Institue of Science and
+Côté travail, j'ai rejoint l'université Okinawa Institute of Science and
 Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, [ouvert
 au public](https://www.oist.jp/unguided-campus-visit) même le week-end (notez
 les horaires du [café](https://www.oist.jp/visitors-center-shops-restaurants#grano)).

updated PO files
diff --git "a/Debian/debi\303\242neries/okinawa.en.po" "b/Debian/debi\303\242neries/okinawa.en.po"
new file mode 100644
index 0000000..6f35f48
--- /dev/null
+++ "b/Debian/debi\303\242neries/okinawa.en.po"
@@ -0,0 +1,68 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2018-09-23 12:34+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Sun, 23 Sep 2018 21:33:11 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Sun, 23 Sep 2018 21:33:11 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"J'ai déménagé à Okinawa !\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier "
+"d'Akano, ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs "
+"[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses "
+"traditionelles faisant usage d'un grand nombre de tambours, ayant souvent "
+"lieu vers la fin de mois d'août.  Chaque quartier a sa propre troupe et nous "
+"espérons pouvoir nous y joindre pour l'année prochaine."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Nous habitons dans un immeuble avec une connection en fibre optique "
+"partagée.  Bon ping vers la métropole, mais le débit pour les gros "
+"téléchargements est catastrophique le soir quand toutes les familles sont "
+"connectées en même temps.  Impossible de réussir un simple `sbuild-update "
+"-dragu unstable`, alors je n'ai malheureusement pas réussi à contribuer qui "
+"que ce soit à Debian.  C'est frustrant mais il y a peut-être des solutions à "
+"chercher du côté de notre [forge GitLab](https://salsa.debian.org)."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Côté travail, j'ai rejoint l'université Okinawa Institue of Science and "
+"Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, "
+"[ouvert au public](https://www.oist.jp/unguided-campus-visit) même le "
+"week-end (notez les horaires du "
+"[café](https://www.oist.jp/visitors-center-shops-restaurants#grano)).  Si "
+"vous venez le visiter, faites-moi signe !"
+msgstr ""

Yasasa
diff --git "a/Debian/debi\303\242neries/okinawa.mdwn" "b/Debian/debi\303\242neries/okinawa.mdwn"
new file mode 100644
index 0000000..b8aaaa7
--- /dev/null
+++ "b/Debian/debi\303\242neries/okinawa.mdwn"
@@ -0,0 +1,26 @@
+[[!meta date="Sun, 23 Sep 2018 21:33:11 +0900"]]
+[[!meta updated="Sun, 23 Sep 2018 21:33:11 +0900"]]
+[[!tag Debian]]
+
+[[!meta title="J'ai déménagé à Okinawa !"]]
+
+J'ai déménagé avec ma famille à Okinawa depuis août, dans le quartier d'Akano,
+ville d'Uruma.  Nous sommes arrivés à temps pour vois plusieurs
+[eisaa](https://en.wikipedia.org/wiki/Eisa_(dance)), des danses traditionelles
+faisant usage d'un grand nombre de tambours, ayant souvent lieu vers la fin de
+mois d'août.  Chaque quartier a sa propre troupe et nous espérons pouvoir nous
+y joindre pour l'année prochaine.
+
+Nous habitons dans un immeuble avec une connection en fibre optique partagée.
+Bon ping vers la métropole, mais le débit pour les gros téléchargements est
+catastrophique le soir quand toutes les familles sont connectées en même temps.
+Impossible de réussir un simple `sbuild-update -dragu unstable`, alors je n'ai
+malheureusement pas réussi à contribuer qui que ce soit à Debian.  C'est
+frustrant mais il y a peut-être des solutions à chercher du côté de notre
+[forge GitLab](https://salsa.debian.org).
+
+Côté travail, j'ai rejoint l'université Okinawa Institue of Science and
+Technology [OIST](https://www.oist.jp).  C'est un endroit formidable, [ouvert
+au public](https://www.oist.jp/unguided-campus-visit) même le week-end (notez
+les horaires du [café](https://www.oist.jp/visitors-center-shops-restaurants#grano)).
+Si vous venez le visiter, faites-moi signe !

Found what "Fol, 1872" stands for.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 5af5724..80701f1 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -18,7 +18,8 @@ Some links:
  - Oikopleura Histone Database: <http://apps.cbu.uib.no/oikohistonedb>
  - Genoscope's genome browser: <http://www.genoscope.cns.fr/externe/GenomeBrowser/Oikopleura/>
  - OikoBase: <http://oikoarrays.biology.uiowa.edu/Oiko/> ([[Danks et al., 2013|biblio/23185044]])
-
+ - Études sur les Appendiculaires du Détroit de Messine, Hermann Fol, 1872
+   ([[ark:/13960/t7fr0vj77|https://archive.org/details/cbarchive_100233_etudessurlesappendiculairesdud1821]]).
 
 Phylogeny
 ---------

Avant café
diff --git a/biblio/30217597.mdwn b/biblio/30217597.mdwn
new file mode 100644
index 0000000..4700286
--- /dev/null
+++ b/biblio/30217597.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Development of the house secreting epithelium, a major innovation of tunicate larvaceans, involves multiple homeodomain transcription factors."]]
+[[!tag Oikopleura]]
+
+Mikhaleva Y, Skinnes R, Sumic S, Thompson EM, Chourrout D.
+
+Dev Biol. 2018 Sep 11. pii: S0012-1606(18)30157-X. doi:10.1016/j.ydbio.2018.09.006
+
+Development of the house secreting epithelium, a major innovation of tunicate larvaceans, involves multiple homeodomain transcription factors.
+
+[[!pmid 30217597 desc="The tandem-duplicated propA and probB genes participate to the development of the house secreting epitelium and control (directly or indirectly) oik41a."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index b026220..5af5724 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -153,6 +153,9 @@ Development
    who noted that it is exceptional in invertebrates, and hypothethise that it may
    be caused by neofunctionalisation (house production, ...) or by the small size of
    the genome (doubling the genes would then double the amount of regulatory sequences).
+ - The tandem _propA_ and _propB_ genes control directly or indirectly _oik41a_
+   and proper development of the house-secreting epithelium ([[Mikhaleva et
+   al., 2018|biblio/30217597]]).
 
 
 Physiology

schpoump
diff --git a/biblio/22343429.mdwn b/biblio/22343429.mdwn
new file mode 100644
index 0000000..893e24b
--- /dev/null
+++ b/biblio/22343429.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research."]]
+[[!tag method nucleus]]
+
+Zhang M, Zhang Y, Scheuring CF, Wu CC, Dong JJ, Zhang HB.
+
+Nat Protoc. 2012 Feb 16;7(3):467-78. doi:10.1038/nprot.2011.455
+
+Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research.
+
+[[!pmid 22343429 desc="Method to produce low melting agarose plugs or beads."]]

Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro.
diff --git a/biblio/26579937.mdwn b/biblio/26579937.mdwn
new file mode 100644
index 0000000..e316361
--- /dev/null
+++ b/biblio/26579937.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro."]]
+[[!tag CRISPR not_read]]
+
+Hinz JM, Laughery MF, Wyrick JJ.
+
+J Biol Chem. 2016 Nov 25;291(48):24851-24856 doi:10.1074/jbc.C116.758706
+
+Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro.
+
+[[!pmid 26579937 desc="Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro."]]

Debranched.
diff --git a/biblio/24213699.mdwn b/biblio/24213699.mdwn
index 7ddff5e..041b09a 100644
--- a/biblio/24213699.mdwn
+++ b/biblio/24213699.mdwn
@@ -7,4 +7,4 @@ Nat Biotechnol. 2013 Dec;31(12):1126-32. doi: 10.1038/nbt.2720
 
 Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells.
 
-[[!pmid 24213699 desc="Tn5 is not efficient on hyperbranched DNA"]]
+[[!pmid 24213699 desc="Tn5 is not efficient on hyperbranched DNA.  Random hexamers and DNA polymerase I were used to debranch the DNA."]]

Old
diff --git a/biblio/28973477.mdwn b/biblio/28973477.mdwn
new file mode 100644
index 0000000..43763bb
--- /dev/null
+++ b/biblio/28973477.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity."]]
+[[!tag method structure]]
+
+Nucleic Acids Res. 2017 Sep 29;45(17):9813-9822. doi:10.1093/nar/gkx765
+
+Einarson OJ, Sen D.
+
+Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity.
+
+[[!pmid 28973477 desc="Could it be turned into a single-cell method ?"]]

Correct tag.
diff --git a/biblio/30154186.mdwn b/biblio/30154186.mdwn
index fe84ccf..896ea95 100644
--- a/biblio/30154186.mdwn
+++ b/biblio/30154186.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler"]]
-[[!tag method sequence_tag nucleus]]
+[[!tag method sequence_tags nucleus]]
 
 J Cell Biol. 2018 Aug 28. pii: jcb.201807108. doi:10.1083/jcb.201807108
 

Prevent smiley.
diff --git a/biblio/30154186.mdwn b/biblio/30154186.mdwn
index ecbc8a1..fe84ccf 100644
--- a/biblio/30154186.mdwn
+++ b/biblio/30154186.mdwn
@@ -7,4 +7,4 @@ Chen Y, Zhang Y, Wang Y, Zhang L, Brinkman EK, Adam SA, Goldman R, van Steensel
 
 Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler 
 
-[[!pmid 30154186 desc="Tyramide free radicals can label DNA directly.  In TSA-Seq (Tyramide Signal Amplification-Sequencing), HRP is targeted on proteins of interest, biotinylated TSA labels neighboring DNA, and biotin-DNA complexes are purified and sequenced.  Distances between chromosome domains and nuclear speckles is reproducible across experiments. Between peripheral lamina (marked by lamin A and B) and nuclear speckles (marked by SON), an increasing gradient of transcriptional activity is clearly visible."]]
+[[!pmid 30154186 desc="Tyramide free radicals can label DNA directly.  In TSA-Seq (Tyramide Signal Amplification-Sequencing), HRP is targeted on proteins of interest, biotinylated TSA labels neighboring DNA, and biotin-DNA complexes are purified and sequenced.  Distances between chromosome domains and nuclear speckles is reproducible across experiments. Between peripheral lamina, marked by lamin A and B, and nuclear speckles (marked by SON), an increasing gradient of transcriptional activity is clearly visible."]]

Old.
diff --git a/biblio/30154186.mdwn b/biblio/30154186.mdwn
new file mode 100644
index 0000000..ecbc8a1
--- /dev/null
+++ b/biblio/30154186.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler"]]
+[[!tag method sequence_tag nucleus]]
+
+J Cell Biol. 2018 Aug 28. pii: jcb.201807108. doi:10.1083/jcb.201807108
+
+Chen Y, Zhang Y, Wang Y, Zhang L, Brinkman EK, Adam SA, Goldman R, van Steensel B, Ma J, Belmont AS.
+
+Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler 
+
+[[!pmid 30154186 desc="Tyramide free radicals can label DNA directly.  In TSA-Seq (Tyramide Signal Amplification-Sequencing), HRP is targeted on proteins of interest, biotinylated TSA labels neighboring DNA, and biotin-DNA complexes are purified and sequenced.  Distances between chromosome domains and nuclear speckles is reproducible across experiments. Between peripheral lamina (marked by lamin A and B) and nuclear speckles (marked by SON), an increasing gradient of transcriptional activity is clearly visible."]]

Old
diff --git a/biblio/29895867.mdwn b/biblio/29895867.mdwn
new file mode 100644
index 0000000..179f50f
--- /dev/null
+++ b/biblio/29895867.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="A photoelectrochemical platform for the capture and release of rare single cells."]]
+[[!tag single_cell microfluidic]]
+
+Parker SG, Yang Y, Ciampi S, Gupta B, Kimpton K, Mansfeld FM, Kavallaris M,
+Gaus K, Gooding JJ.
+
+Nat Commun. 2018 Jun 12;9(1):2288. doi:10.1038/s41467-018-04701-y
+
+A photoelectrochemical platform for the capture and release of rare single cells.
+
+[[!pmid 29895867 desc="Cells captured by an antibody by an electrochemically cleavable linker."]]

Normalisation.
diff --git a/biblio/21596820.mdwn b/biblio/21596820.mdwn
index 340bdb9..47716c3 100644
--- a/biblio/21596820.mdwn
+++ b/biblio/21596820.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Unamplified cap analysis of gene expression on a single-molecule sequencer."]]
-[[!tag CAGE Helicos sequence_tag method]]
+[[!tag CAGE Helicos sequence_tags method]]
 
 Genome Res. 2011 Jul;21(7):1150-9. doi:10.1101/gr.115469.110
 
diff --git a/tags/sequence_tag.mdwn b/tags/sequence_tag.mdwn
deleted file mode 100644
index 244d1cd..0000000
--- a/tags/sequence_tag.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged sequence tag"]]
-
-[[!inline pages="tagged(sequence_tag)" actions="no" archive="yes"
-feedshow=10]]

Old
diff --git a/biblio/28973448.mdwn b/biblio/28973448.mdwn
new file mode 100644
index 0000000..6fafeed
--- /dev/null
+++ b/biblio/28973448.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers."]]
+[[!tag sequence_tags epigenetic method]]
+
+Nucleic Acids Res. 2017 Sep 29;45(17):e153. doi:10.1093/nar/gkx648
+
+Zarnegar MA, Reinitz F, Newman AM, Clarke MF.
+
+Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers.
+
+[[!pmid 28973448 desc="Proximity ligation between DNA ends and antibody-linked adaptors."]]

Café
diff --git a/biblio/17829536.mdwn b/biblio/17829536.mdwn
new file mode 100644
index 0000000..d70c5ed
--- /dev/null
+++ b/biblio/17829536.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Zooplankton Species Groups in the North Pacific"]]
+[[!tag richness statistics]]
+
+Science. 1963 May 3;140(3566):453-60 doi:10.1126/science.140.3566.453
+
+Fager EW, McGowan JA.
+
+Zooplankton Species Groups in the North Pacific
+
+[[!pmid 17829536 desc="Fager & McGowan index of affinity: [J/√(Na.Nb)] - 1/2√Nb, where J is number of joined occurences and Na and Nb are the number of occurences of a and b respectively.  Nb is chosen to be greater or equal to Na.  Scores higher than 0.5 denote affinity.  The paper then explores the geographical distribution of groups of species, where all species in the same group have affinity for each other."]]

Splice leader sequence.
diff --git a/biblio/15314184.mdwn b/biblio/15314184.mdwn
index 2ca7c3e..b45e7b3 100644
--- a/biblio/15314184.mdwn
+++ b/biblio/15314184.mdwn
@@ -7,4 +7,4 @@ Mol Cell Biol. 2004 Sep;24(17):7795-805. doi:10.1128/MCB.24.17.7795-7805.2004
 
 Spliced-Leader RNA trans Splicing in a Chordate, Oikopleura dioica, with a Compact Genome.
 
-[[!pmid 15314184 desc="5′ splice leader (SL) found in 90/158 ESTs containing a start codon.  The SL RNA is found downstream of the 5S RNA in at least 40 occurences, and aproximately 2/3 of all the 5S rRNA genes.  O. doica is the first chordate where gene operons have been described."]]
+[[!pmid 15314184 desc="5′ splice leader (SL) found in 90/158 ESTs containing a start codon.  The SL RNA is found downstream of the 5S RNA in at least 40 occurences, and aproximately 2/3 of all the 5S rRNA genes.  Its sequence is ACTCATCCCATTTTTGAGTCCGATTTCGATTGTCTAACAG. O. doica is the first chordate where gene operons have been described."]]

Old
diff --git a/biblio/29320473.mdwn b/biblio/29320473.mdwn
new file mode 100644
index 0000000..e7a9587
--- /dev/null
+++ b/biblio/29320473.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Systems of mechanized and reactive droplets powered by multi-responsive surfactants."]]
+[[!tag emulsion]]
+
+Nature. 2018 Jan 18;553(7688):313-318. doi:10.1038/nature25137
+
+Yang Z, Wei J, Sobolev YI, Grzybowski BA.
+
+Systems of mechanized and reactive droplets powered by multi-responsive surfactants.
+
+[[!pmid 29320473 desc="New amphiphilic surfactants containing gold, iron oxide or lead sulfide were used to create droplets that aggregate in magnetic fields, translate or rotate under laser illumination, and weld together electric fields. Combining these three manipulations allowed for the creation of complex structures used as microreactors for chemical reactions between reagents brought by individual droplets."]]
diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index b72e5ee..dc83bbe 100644
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -21,4 +21,8 @@ Different kinds of surfactants:
  - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]])
  - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]
 
+Reactive droplets:
+
+ - Illumination changing magnetic or electric properties of droplets ([[Yang et al., 2018|biblio/29320473]]).
+
 [[!inline pages="tagged(emulsion)" actions="no" limit=0]]

Link to BOLD.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index a21d1f5..b026220 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -191,5 +191,7 @@ Ecology
    population size” ([[Denoeud et al., 2010|biblio/21097902]], according to a study
    of silent and non-silent substitution rates in coding sequences).
  - It was already reported to be frequent in Japanese waters [[in 1907 by T. Aida|biblio/AA0069577]].
+ - As of August 2018, _O. dioica_ is not yet found in the [BOLD](http://www.boldsystems.org/)
+   database of DNA barcodes.
 
 [[!inline pages="tagged(Oikopleura)" actions="no" limit=0]]

Tiling.
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index b16538d..a21d1f5 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -17,7 +17,7 @@ Some links:
 
  - Oikopleura Histone Database: <http://apps.cbu.uib.no/oikohistonedb>
  - Genoscope's genome browser: <http://www.genoscope.cns.fr/externe/GenomeBrowser/Oikopleura/>
- - OikoBase: <http://oikoarrays.biology.uiowa.edu/Oiko/>
+ - OikoBase: <http://oikoarrays.biology.uiowa.edu/Oiko/> ([[Danks et al., 2013|biblio/23185044]])
 
 
 Phylogeny
@@ -131,6 +131,7 @@ Transcriptome
    by reverse splicing, ([[Denoeud et al., 2010|biblio/21097902]]).  Larger introns tend
    to be older, and among the large introns, the older contain repeat elements less frequently
    than the newer ([[Denoeud et al., 2010|biblio/21097902]]). 
+ - 12 developmental stages were studied with tiling arrays by [[Danks et al., 2013|biblio/23185044]].
 
 
 Tools

Tiling array.
diff --git a/biblio/23185044.mdwn b/biblio/23185044.mdwn
new file mode 100644
index 0000000..b5e9c64
--- /dev/null
+++ b/biblio/23185044.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="OikoBase: a genomics and developmental transcriptomics resource for the urochordate Oikopleura dioica."]]
+[[!tag Oikopleura]]
+
+Nucleic Acids Res. 2013 Jan;41(Database issue):D845-53. doi:10.1093/nar/gks1159
+
+Danks G, Campsteijn C, Parida M, Butcher S, Doddapaneni H, Fu B, Petrin R, Metpally R, Lenhard B, Wincker P, Chourrout D, Thompson EM, Manak JR.
+
+OikoBase: a genomics and developmental transcriptomics resource for the urochordate Oikopleura dioica.
+
+[[!pmid 23185044 desc="Tiling array expression data for 12 developmental stages."]]

Café.
diff --git a/biblio/1929090.mdwn b/biblio/1929090.mdwn
new file mode 100644
index 0000000..48feb89
--- /dev/null
+++ b/biblio/1929090.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="The Seasonal Distribution of the Appendicularia in the Region of San Diego, California"]]
+[[!tag Oikopleura]]
+
+Ecology Vol. 3, No. 1 (Jan., 1922), pp. 55-64
+
+Christine E. Essenberg
+
+The Seasonal Distribution of the Appendicularia in the Region of San Diego, California
+
+[[!doi 10.2307/1929090 desc="Higher abundances of O. dioica in cool temperatures during winter."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index a27282b..b16538d 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -183,6 +183,9 @@ Ecology
 
  - _O. dioica_ populations may have a higher fitness in warmer and more acid oceans
    ([[Bouquet et al., 2018|biblio/29298334]]).
+ - In California, _O. dioica_ was reported by C. Essenberg
+   ([[1922|biblio/1929090]]) to be rare in summer's warm (> 20ºC) waters and
+   more abundant in winter's cool (13~16 ºC) waters.
  - _O. dioica_ has “a very large mutation rate, and/or a very large effective
    population size” ([[Denoeud et al., 2010|biblio/21097902]], according to a study
    of silent and non-silent substitution rates in coding sequences).

Methylation.
diff --git a/biblio/28115992.mdwn b/biblio/28115992.mdwn
index 3a7c736..8c32193 100644
--- a/biblio/28115992.mdwn
+++ b/biblio/28115992.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Sex-specific chromatin landscapes in an ultra-compact chordate genome."]]
-[[!tag Oikopleura epigenetic]]
+[[!tag Oikopleura methylation epigenetic]]
 
 Navratilova P, Danks GB, Long A, Butcher S, Manak JR, Thompson EM.
 
@@ -7,4 +7,4 @@ Epigenetics Chromatin. 2017 Jan 17;10:3. doi:10.1186/s13072-016-0110-4
 
 Sex-specific chromatin landscapes in an ultra-compact chordate genome.
 
-[[!pmid 28115992 desc="Chromatin domains usually smaller than 7 nucleosomes."]]
+[[!pmid 28115992 desc="Chromatin domains usually smaller than 7 nucleosomes.  5-methylcytosine detected by immunoprecipitation and microarray analysis (MeDIP-chip)."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index dfb3fe6..a27282b 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -55,6 +55,7 @@ Genome
    level of conserved synteny at larger distances.” ([[Denoeud et al.,
    2010|biblio/21097902]])
  - Chromatin domains are rarely longer than 7 nucleosomes ([[Navratilova et al., 2017|biblio/28115992]]).
+ - Some 5-methylcytosine was detected by MeDIP-chip by ([[Navratilova et al., 2017|biblio/28115992]]).
  - The entire machinery required for performing NHEJ repair of DSB (which is
    conserved from yeast to mammals) is undetectable ([[Denoeud et al., 2010|biblio/21097902]]).
  - Only a partial mitochondrial genome was reconstituted in [[Denoeud et al.,

Normalisation.
diff --git a/biblio/29291348.mdwn b/biblio/29291348.mdwn
index 3a713b6..7225a5c 100644
--- a/biblio/29291348.mdwn
+++ b/biblio/29291348.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias."]]
-[[!tag fingerprint transcriptomics]]
+[[!tag fingerprint transcriptome]]
 
 Karst SM, Dueholm MS, McIlroy SJ, Kirkegaard RH, Nielsen PH, Albertsen M.
 

Au bureau
diff --git a/biblio/29945865.mdwn b/biblio/29945865.mdwn
new file mode 100644
index 0000000..3ba0182
--- /dev/null
+++ b/biblio/29945865.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting."]]
+[[!tag transcriptome cell_type]]
+
+Development. 2018 Jul 11;145(13). pii: dev164640. doi:10.1242/dev.164640
+
+Matsushima W, Herzog VA, Neumann T, Gapp K, Zuber J, Ameres SL, Miska EA.
+
+SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting.
+
+[[!pmid 29945865 desc="SLAMseq in tissue (SLAM-ITseq).  Tissue-specific UPRT expression selectively incorporates 4-thiouracil in RNA.  Alkylation with iodoacetamide (IAA) leads to G → A mutations during reverse transcription, which are detected and quantified during data analysis."]]

Café encore.
diff --git a/biblio/29986741.mdwn b/biblio/29986741.mdwn
new file mode 100644
index 0000000..6c81d5b
--- /dev/null
+++ b/biblio/29986741.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Splicing heterogeneity: separating signal from noise."]]
+[[!tag splicing review single_cell]]
+
+Genome Biol. 2018 Jul 9;19(1):86. doi:10.1186/s13059-018-1467-4
+
+Wan Y, Larson DR.
+
+Splicing heterogeneity: separating signal from noise.
+
+[[!pmid 29986741 desc="Review covering splicing at the single-cell level"]]

Café
diff --git a/biblio/3138163.mdwn b/biblio/3138163.mdwn
new file mode 100644
index 0000000..b753818
--- /dev/null
+++ b/biblio/3138163.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Splice site selection, rate of splicing, and alternative splicing on nascent transcripts."]]
+[[!tag Drosophila splicing]]
+
+Genes Dev. 1988 Jun;2(6):754-65.
+
+Beyer AL, Osheim YN.
+
+Splice site selection, rate of splicing, and alternative splicing on nascent transcripts.
+
+[[!pmid 3138163 desc="Chromatin spread illustrating alternative splicing."]]

Precisions
diff --git a/biblio/27897270.mdwn b/biblio/27897270.mdwn
index a3048b3..431ea4e 100644
--- a/biblio/27897270.mdwn
+++ b/biblio/27897270.mdwn
@@ -7,4 +7,4 @@ Nat Commun. 2016 Nov 29;7:13296. doi:10.1038/ncomms13296
 
 TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.
 
-[[!pmid 27897270 desc="Isothermal amplification for 3 h at 30 °C using a mixture of TthPrimPol for priming and and Φ29DNApol for extension."]]
+[[!pmid 27897270 desc="Isothermal amplification for 3 h at 30 °C using a mixture of TthPrimPol for priming and and Φ29DNApol for extension.  Target DNA was first denatured in an alkaline buffer."]]

Ketchup
diff --git a/biblio/27897270.mdwn b/biblio/27897270.mdwn
new file mode 100644
index 0000000..a3048b3
--- /dev/null
+++ b/biblio/27897270.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol."]]
+[[!tag method amplification enzyme primase]]
+
+Picher ÁJ, Budeus B, Wafzig O, Krüger C, García-Gómez S, Martínez-Jiménez MI, Díaz-Talavera A, Weber D, Blanco L, Schneider A.
+
+Nat Commun. 2016 Nov 29;7:13296. doi:10.1038/ncomms13296
+
+TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.
+
+[[!pmid 27897270 desc="Isothermal amplification for 3 h at 30 °C using a mixture of TthPrimPol for priming and and Φ29DNApol for extension."]]

creating tag page tags/primase
diff --git a/tags/primase.mdwn b/tags/primase.mdwn
new file mode 100644
index 0000000..2b277c1
--- /dev/null
+++ b/tags/primase.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged primase"]]
+
+[[!inline pages="tagged(primase)" actions="no" archive="yes"
+feedshow=10]]

Prima
diff --git a/biblio/28911121.mdwn b/biblio/28911121.mdwn
index b30a1ab..e10b3df 100644
--- a/biblio/28911121.mdwn
+++ b/biblio/28911121.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase."]]
-[[!tag enzyme reverse_transcription]]
+[[!tag enzyme primase reverse_transcription]]
 
 Nucleic Acids Res. 2017 Sep 6;45(15):9046-9058. doi:10.1093/nar/gkx633
 

Café
diff --git a/biblio/30005597.mdwn b/biblio/30005597.mdwn
new file mode 100644
index 0000000..7969492
--- /dev/null
+++ b/biblio/30005597.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy."]]
+[[!tag nanopore review]]
+
+Genome Biol. 2018 Jul 13;19(1):90. doi:10.1186/s13059-018-1462-9
+
+Rang FJ, Kloosterman WP, de Ridder J.
+
+From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy.
+
+[[!pmid 30005597 desc="From R6 to R9.5."]]

Cleanup encore
diff --git a/tags/rna-seq.mdwn b/tags/rna-seq.mdwn
deleted file mode 100644
index bba2247..0000000
--- a/tags/rna-seq.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged rna-seq"]]
-
-[[!inline pages="tagged(rna-seq)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/rna.mdwn b/tags/rna.mdwn
deleted file mode 100644
index 36d49c2..0000000
--- a/tags/rna.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged rna"]]
-
-[[!inline pages="tagged(rna)" actions="no" archive="yes"
-feedshow=10]]

Cleanup.
diff --git a/biblio/17373481.mdwn b/biblio/17373481.mdwn
index ce7e81d..e4568cf 100644
--- a/biblio/17373481.mdwn
+++ b/biblio/17373481.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="5′-tailed sequencing primers improve sequencing quality of PCR products."]]
-[[!tag sanger sequencing]]
+[[!tag Sanger sequencing]]
 [[!pmid 17373481 desc="A long tail (40 bp, for instance) increases the size of the fragments to separate on gel, and therefore resolution in 5′"]]
diff --git a/tags/RNA-Seq.mdwn b/tags/RNA-Seq.mdwn
deleted file mode 100644
index bfbf634..0000000
--- a/tags/RNA-Seq.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged RNA-Seq"]]
-
-[[!inline pages="tagged(RNA-Seq)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/heliscope.mdwn b/tags/heliscope.mdwn
deleted file mode 100644
index f1764df..0000000
--- a/tags/heliscope.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged heliscope"]]
-
-[[!inline pages="tagged(heliscope)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/parkinson.mdwn b/tags/parkinson.mdwn
deleted file mode 100644
index 0502e73..0000000
--- a/tags/parkinson.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged parkinson"]]
-
-[[!inline pages="tagged(parkinson)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/sanger.mdwn b/tags/sanger.mdwn
deleted file mode 100644
index c4e7100..0000000
--- a/tags/sanger.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged sanger"]]
-
-[[!inline pages="tagged(sanger)" actions="no" archive="yes"
-feedshow=10]]
diff --git a/tags/tfbs.mdwn b/tags/tfbs.mdwn
deleted file mode 100644
index 984b40b..0000000
--- a/tags/tfbs.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged tfbs"]]
-
-[[!inline pages="tagged(tfbs)" actions="no" archive="yes"
-feedshow=10]]

BUSCO
diff --git a/biblio/26059717.mdwn b/biblio/26059717.mdwn
new file mode 100644
index 0000000..7e725d2
--- /dev/null
+++ b/biblio/26059717.mdwn
@@ -0,0 +1,8 @@
+[[!meta title="BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs."]]
+[[!tag assembly]]
+
+Bioinformatics. 2015 Oct 1;31(19):3210-2. doi:10.1093/bioinformatics/btv351
+
+BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.
+
+[[!pmid 26059717 desc="Benchmarking Universal Single-Copy Orthologs. Can be used to train predictors such as Augustus."]]
diff --git a/biblio/29220515.mdwn b/biblio/29220515.mdwn
new file mode 100644
index 0000000..342e62b
--- /dev/null
+++ b/biblio/29220515.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="BUSCO applications from quality assessments to gene prediction and phylogenomics."]]
+[[!tag assembly]]
+
+Waterhouse RM, Seppey M, Simão FA, Manni M, Ioannidis P, Klioutchnikov G, Kriventseva EV, Zdobnov EM.
+
+Mol Biol Evol. 2017 Dec 6. doi:10.1093/molbev/msx319
+
+BUSCO applications from quality assessments to gene prediction and phylogenomics.
+
+[[!pmid 29220515 desc="BUSCO v3 and examples of use."]]

Normalise.
diff --git a/biblio/30018084.mdwn b/biblio/30018084.mdwn
index 15291cb..dc0ce8c 100644
--- a/biblio/30018084.mdwn
+++ b/biblio/30018084.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing."]]
-[[!tag Drosophila Nanopore assembly]]
+[[!tag Drosophila nanopore assembly]]
 
 G3 (Bethesda). 2018 Jul 17. pii: g3.200162.2018. doi:10.1534/g3.118.200162
 
diff --git a/biblio/30087105.mdwn b/biblio/30087105.mdwn
index ed9f085..e0bb1a0 100644
--- a/biblio/30087105.mdwn
+++ b/biblio/30087105.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Highly Contiguous Genome Assemblies of 15 Drosophila Species Generated Using Nanopore Sequencing."]]
-[[!tag Drosophila Nanopore assembly]]
+[[!tag Drosophila nanopore assembly]]
 
 G3 (Bethesda). 2018 Aug 7. pii: g3.200160.2018. doi:10.1534/g3.118.200160
 

ketchup
diff --git a/biblio/30087105.mdwn b/biblio/30087105.mdwn
new file mode 100644
index 0000000..ed9f085
--- /dev/null
+++ b/biblio/30087105.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Highly Contiguous Genome Assemblies of 15 Drosophila Species Generated Using Nanopore Sequencing."]]
+[[!tag Drosophila Nanopore assembly]]
+
+G3 (Bethesda). 2018 Aug 7. pii: g3.200160.2018. doi:10.1534/g3.118.200160
+
+Miller DE, Staber C, Zeitlinger J, Hawley RS.
+
+Highly Contiguous Genome Assemblies of 15 Drosophila Species Generated Using Nanopore Sequencing.
+
+[[!pmid 30087105 desc="29× coverage and N50 of 4.4. Mb in average.  A multiplexed NextSeq 500 run was used for polishing.  Optimisation of Nanopore throughput by reorganising pore groups periodically, extracting high molecular weight DNA with phenol/chloroform extration, and using more DNA in the library preparation.  Benchmark of various tools including minimap/miniasm and canu."]]

Merge branch 'master' of ssh://charles-plessy-org.branchable.com
Mot manquant
diff --git "a/Debian/debi\303\242neries/imprimanteBrother.en.po" "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
index d9f3c39..d515191 100644
--- "a/Debian/debi\303\242neries/imprimanteBrother.en.po"
+++ "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2018-08-22 12:55+0000\n"
-"PO-Revision-Date: 2018-08-22 21:59+0900\n"
+"PO-Revision-Date: 2018-08-22 22:01+0900\n"
 "Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
@@ -47,9 +47,10 @@ msgid ""
 "tout semble se passer parfaitement sauf que rien ne sort de l'imprimante."
 msgstr ""
 "I ran into circles for more than one hour, before eventually understanding "
-"that one needs to install the `lib32stdc++6` in order to use the Brother "
-"drivers (HL-L2365DW printer) on an `amd64` system, since they are provided "
-"as `i386` packages. Only after, I realised that there was more than a hint "
-"in the [online instructions](http://support.brother.com/g/s/id/linux/en/"
-"before.html).  The hardest part was that without `lib32stdc++6`, everything "
-"seemed to work fine, except that nothing was coming out from the printer."
+"that one needs to install the `lib32stdc++6` package in order to use the "
+"Brother drivers (HL-L2365DW printer) on an `amd64` system, since they are "
+"provided as `i386` packages. Only after, I realised that there was more "
+"than a hint in the [online instructions](http://support.brother.com/g/s/id/"
+"linux/en/before.html).  The hardest part was that without `lib32stdc++6`, "
+"everything seemed to work fine, except that nothing was coming out from the "
+"printer."

updated PO files
diff --git "a/Debian/debi\303\242neries/imprimanteBrother.en.po" "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
index d9f3c39..2534769 100644
--- "a/Debian/debi\303\242neries/imprimanteBrother.en.po"
+++ "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
@@ -6,14 +6,14 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2018-08-22 12:55+0000\n"
+"POT-Creation-Date: 2018-08-22 13:00+0000\n"
 "PO-Revision-Date: 2018-08-22 21:59+0900\n"
+"Last-Translator: Charles Plessy <toto@example.com>\n"
+"Language-Team: \n"
 "Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
-"Last-Translator: Charles Plessy <toto@example.com>\n"
-"Language-Team: \n"
 "X-Generator: Poedit 1.8.11\n"
 
 #. type: Plain text

Brother
diff --git "a/Debian/debi\303\242neries/imprimanteBrother.en.po" "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
index 9abb6d7..d9f3c39 100644
--- "a/Debian/debi\303\242neries/imprimanteBrother.en.po"
+++ "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
@@ -3,38 +3,38 @@
 # This file is distributed under the same license as the PACKAGE package.
 # FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
 #
-#, fuzzy
 msgid ""
 msgstr ""
-"Project-Id-Version: PACKAGE VERSION\n"
+"Project-Id-Version: \n"
 "POT-Creation-Date: 2018-08-22 12:55+0000\n"
-"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
-"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
-"Language-Team: LANGUAGE <LL@li.org>\n"
-"Language: \n"
+"PO-Revision-Date: 2018-08-22 21:59+0900\n"
+"Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
+"Last-Translator: Charles Plessy <toto@example.com>\n"
+"Language-Team: \n"
+"X-Generator: Poedit 1.8.11\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta date=\"Wed, 22 Aug 2018 21:41:32 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta date=\"Wed, 22 Aug 2018 21:41:32 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta updated=\"Wed, 22 Aug 2018 21:41:32 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta updated=\"Wed, 22 Aug 2018 21:41:32 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!tag Debian]]\n"
-msgstr ""
+msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta title=\"Imprimer avec Brother sur Debian\"]]\n"
-msgstr ""
+msgstr "[[!meta title=\"Print with Brother from Debian\"]]\n"
 
 #. type: Plain text
 msgid ""
@@ -42,8 +42,14 @@ msgid ""
 "qu'il faut aussi installer le paquet `lib32stdc++6` quand on utilise les "
 "pilotes Brother (imprimante HL-L2365DW) sur un système `amd64`, puisqu'ils "
 "sont fournis sous la forme de paquets `i386`.  Pourtant il y a plus d'une "
-"piste dans les [instructions en "
-"ligne](http://support.brother.com/g/s/id/linux/en/before.html).  Le plus "
-"difficile était que sans `lib32stdc++6`, tout semble se passer parfaitement "
-"sauf que rien ne sort de l'imprimante."
+"piste dans les [instructions en ligne](http://support.brother.com/g/s/id/"
+"linux/en/before.html).  Le plus difficile était que sans `lib32stdc++6`, "
+"tout semble se passer parfaitement sauf que rien ne sort de l'imprimante."
 msgstr ""
+"I ran into circles for more than one hour, before eventually understanding "
+"that one needs to install the `lib32stdc++6` in order to use the Brother "
+"drivers (HL-L2365DW printer) on an `amd64` system, since they are provided "
+"as `i386` packages. Only after, I realised that there was more than a hint "
+"in the [online instructions](http://support.brother.com/g/s/id/linux/en/"
+"before.html).  The hardest part was that without `lib32stdc++6`, everything "
+"seemed to work fine, except that nothing was coming out from the printer."

updated PO files
diff --git "a/Debian/debi\303\242neries/imprimanteBrother.en.po" "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
new file mode 100644
index 0000000..9abb6d7
--- /dev/null
+++ "b/Debian/debi\303\242neries/imprimanteBrother.en.po"
@@ -0,0 +1,49 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2018-08-22 12:55+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Wed, 22 Aug 2018 21:41:32 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Wed, 22 Aug 2018 21:41:32 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Imprimer avec Brother sur Debian\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"J'ai tourné en rond pendant plus d'une heure, avant de finir par comprendre "
+"qu'il faut aussi installer le paquet `lib32stdc++6` quand on utilise les "
+"pilotes Brother (imprimante HL-L2365DW) sur un système `amd64`, puisqu'ils "
+"sont fournis sous la forme de paquets `i386`.  Pourtant il y a plus d'une "
+"piste dans les [instructions en "
+"ligne](http://support.brother.com/g/s/id/linux/en/before.html).  Le plus "
+"difficile était que sans `lib32stdc++6`, tout semble se passer parfaitement "
+"sauf que rien ne sort de l'imprimante."
+msgstr ""

Grand frère II.
diff --git "a/Debian/debi\303\242neries/imprimanteBrother.mdwn" "b/Debian/debi\303\242neries/imprimanteBrother.mdwn"
new file mode 100644
index 0000000..c47656b
--- /dev/null
+++ "b/Debian/debi\303\242neries/imprimanteBrother.mdwn"
@@ -0,0 +1,13 @@
+[[!meta date="Wed, 22 Aug 2018 21:41:32 +0900"]]
+[[!meta updated="Wed, 22 Aug 2018 21:41:32 +0900"]]
+[[!tag Debian]]
+
+[[!meta title="Imprimer avec Brother sur Debian"]]
+
+J'ai tourné en rond pendant plus d'une heure, avant de finir par comprendre
+qu'il faut aussi installer le paquet `lib32stdc++6` quand on utilise les
+pilotes Brother (imprimante HL-L2365DW) sur un système `amd64`, puisqu'ils sont
+fournis sous la forme de paquets `i386`.  Pourtant il y a plus d'une piste dans
+les [instructions en ligne](http://support.brother.com/g/s/id/linux/en/before.html).
+Le plus difficile était que sans `lib32stdc++6`, tout semble se passer parfaitement
+sauf que rien ne sort de l'imprimante.

Droso
diff --git a/biblio/30018084.mdwn b/biblio/30018084.mdwn
index 51ea447..15291cb 100644
--- a/biblio/30018084.mdwn
+++ b/biblio/30018084.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing."]]
-[[!tag Nanopore assembly]]
+[[!tag Drosophila Nanopore assembly]]
 
 G3 (Bethesda). 2018 Jul 17. pii: g3.200162.2018. doi:10.1534/g3.118.200162
 

Normalisation.
diff --git a/biblio/20615941.mdwn b/biblio/20615941.mdwn
index 694c505..5a28f17 100644
--- a/biblio/20615941.mdwn
+++ b/biblio/20615941.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="Global analysis of trans-splicing in Drosophila."]]
-[[!tag drosophila trans-splicing]]
+[[!tag Drosophila trans-splicing]]
 [[!pmid 20615941 desc="Hybrid experiment found cases of trans-splicing, but few."]]
diff --git a/biblio/20868489.mdwn b/biblio/20868489.mdwn
index 096fdae..bfc37a0 100644
--- a/biblio/20868489.mdwn
+++ b/biblio/20868489.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="An arthropod cis-regulatory element functioning in sensory organ precursor development dates back to the Cambrian."]]
-[[!tag enhancer sequence-conservation drosophila]]
+[[!tag enhancer sequence-conservation Drosophila]]
 [[!pmid 20868489 desc="The atonal sensory organ precursor enhancer (SOPE)"]]
diff --git a/biblio/23537633.mdwn b/biblio/23537633.mdwn
index 94ec47a..377c760 100644
--- a/biblio/23537633.mdwn
+++ b/biblio/23537633.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="A Sequence in the Drosophila H3-H4 Promoter Triggers Histone Locus Body Assembly and Biosynthesis of Replication-Coupled Histone mRNAs."]]
-[[!tag histone nuclear_body promoter bidirectional drosophila ]]
+[[!tag histone nuclear_body promoter bidirectional Drosophila ]]
 
 Salzler HR, Tatomer DC, Malek PY, McDaniel SL, Orlando AN, Marzluff WF, Duronio RJ.
 
diff --git a/biblio/24631403.mdwn b/biblio/24631403.mdwn
index fdda40c..2a240b5 100644
--- a/biblio/24631403.mdwn
+++ b/biblio/24631403.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Dedifferentiation of Neurons Precedes Tumor Formation in lola Mutants."]]
-[[!tag drosophila cell_cycle]]
+[[!tag Drosophila cell_cycle]]
 
 Southall TD, Davidson CM, Miller C, Carr A, Brand AH.
 
diff --git a/biblio/26098021.mdwn b/biblio/26098021.mdwn
index 29639b7..92c76b6 100644
--- a/biblio/26098021.mdwn
+++ b/biblio/26098021.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Combining protein and mRNA quantification to decipher transcriptional regulation."]]
-[[!tag single_cell transcription_factor imaging drosophila]]
+[[!tag single_cell transcription_factor imaging Drosophila]]
 
 Xu H, Sepúlveda LA, Figard L, Sokac AM, Golding I.
 
diff --git a/biblio/26680200.mdwn b/biblio/26680200.mdwn
index 55c27dc..1233ffb 100644
--- a/biblio/26680200.mdwn
+++ b/biblio/26680200.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="An essential cell cycle regulation gene causes hybrid inviability in Drosophila."]]
-[[!tag drosophila cell_cycle]]
+[[!tag Drosophila cell_cycle]]
 
 Phadnis N, Baker EP, Cooper JC, Frizzell KA, Hsieh E, de la Cruz AF, Shendure 
 J, Kitzman JO, Malik HS.
diff --git a/tags/drosophila.mdwn b/tags/drosophila.mdwn
deleted file mode 100644
index 619c2eb..0000000
--- a/tags/drosophila.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged drosophila"]]
-
-[[!inline pages="tagged(drosophila)" actions="no" archive="yes"
-feedshow=10]]

Typo.
diff --git a/biblio/29934612.mdwn b/biblio/29934612.mdwn
index 26b5948..2df471a 100644
--- a/biblio/29934612.mdwn
+++ b/biblio/29934612.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Experimental evidence that thermal selection shapes mitochondrial genome evolution."]]
-[[!tag Drosophia mitochondrion evolution temperature]]
+[[!tag Drosophila mitochondrion evolution temperature]]
 
 Sci Rep. 2018 Jun 22;8(1):9500. doi:10.1038/s41598-018-27805-3
 
diff --git a/tags/Drosophia.mdwn b/tags/Drosophia.mdwn
deleted file mode 100644
index fb8b636..0000000
--- a/tags/Drosophia.mdwn
+++ /dev/null
@@ -1,4 +0,0 @@
-[[!meta title="pages tagged Drosophia"]]
-
-[[!inline pages="tagged(Drosophia)" actions="no" archive="yes"
-feedshow=10]]

creating tag page tags/Drosophia
diff --git a/tags/Drosophia.mdwn b/tags/Drosophia.mdwn
new file mode 100644
index 0000000..fb8b636
--- /dev/null
+++ b/tags/Drosophia.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged Drosophia"]]
+
+[[!inline pages="tagged(Drosophia)" actions="no" archive="yes"
+feedshow=10]]

Café
diff --git a/biblio/29934612.mdwn b/biblio/29934612.mdwn
new file mode 100644
index 0000000..26b5948
--- /dev/null
+++ b/biblio/29934612.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Experimental evidence that thermal selection shapes mitochondrial genome evolution."]]
+[[!tag Drosophia mitochondrion evolution temperature]]
+
+Sci Rep. 2018 Jun 22;8(1):9500. doi:10.1038/s41598-018-27805-3
+
+Lajbner Z, Pnini R, Camus MF, Miller J, Dowling DK.
+
+Experimental evidence that thermal selection shapes mitochondrial genome evolution.
+
+[[!pmid 29934612 desc="Experimental evidence for the “mitochondrial climatic hypothesis”.  Haplotypes only differ by non-coding or synonymous changes.  Wolbachia infection was a confounding factor and needed to be removed by antibiotic treatment."]]