Dernières modifications :

Tag.
diff --git a/biblio/24079827.mdwn b/biblio/24079827.mdwn
index 1687532..3349ba7 100644
--- a/biblio/24079827.mdwn
+++ b/biblio/24079827.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Comparison of RNA- or LNA-hybrid oligonucleotides in template-switching reactions for high-speed sequencing library preparation."]]
-[[!tag template_switching CAGE OSC]]
+[[!tag template_switching CAGE OSC LNA]]
 
 Harbers M, Kato S, de Hoon M, Hayashizaki Y, Carninici P, Plessy C.
 

From the past.
diff --git a/biblio/12703294.mdwn b/biblio/12703294.mdwn
new file mode 100644
index 0000000..7601771
--- /dev/null
+++ b/biblio/12703294.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Modified 3'-end amplification PCR for gene expression analysis in single cells."]]
+[[!tag single_cell qPCR]]
+
+Heams T, Kupiec JJ.
+
+Biotechniques. 2003 Apr;34(4):712-4, 716.
+
+Modified 3'-end amplification PCR for gene expression analysis in single cells.
+
+[[!pmid 12703294 desc="12703294 Improvement of TPEA method. Gets rid of the nucleus by centrifugation."]]
diff --git a/biblio/12736331.mdwn b/biblio/12736331.mdwn
new file mode 100644
index 0000000..3bfd101
--- /dev/null
+++ b/biblio/12736331.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity."]]
+[[!tag single_cell microarray heterogeneity]]
+
+Kamme F, Salunga R, Yu J, Tran DT, Zhu J, Luo L, Bittner A, Guo HQ, Miller N, Wan J, Erlander M.
+
+J Neurosci. 2003 May 1;23(9):3607-15.
+
+Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.
+
+[[!pmid 12736331 desc="Proof of concept and method, from Johnson & Johnson, Acturus, and OmniViz."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 3280279..ab2aa66 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -107,15 +107,9 @@ Oligo-capping to fine-map transcription start sites.
 14606961 [libraries]
 The nuclease activity of T7 RNA pol could also show some transcript specificity.
 
-12736331 [single cell]
-Proof of concept and method, from Johnson & Johnson, Acturus, and OmniViz.
-
 14704353 [libraries] [tracked]
 T4 DNA ligase used to ligate a double stranded cap-tag.
 
-12703294 [single cells]
-Improvement of TPEA method. Gets rid of the nucleus by centrifugation.
-
 11730011 [libraries]
 Type IIS restriction digest followed py 3'primer ligation, to rempve the poly A from cDNA.
 

creating tag page tags/ageing
diff --git a/tags/ageing.mdwn b/tags/ageing.mdwn
new file mode 100644
index 0000000..ceefdeb
--- /dev/null
+++ b/tags/ageing.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged ageing"]]
+
+[[!inline pages="tagged(ageing)" actions="no" archive="yes"
+feedshow=10]]

Hop
diff --git a/biblio/28746310.mdwn b/biblio/28746310.mdwn
new file mode 100644
index 0000000..469bf32
--- /dev/null
+++ b/biblio/28746310.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Hypothalamic stem cells control ageing speed partly through exosomal miRNAs."]]
+[[!tag not_read exosome ageing mouse]]
+
+Nature. 2017 Jul 26. doi:10.1038/nature23282
+
+Zhang Y, Kim MS, Jia B, Yan J, Zuniga-Hertz JP, Han C, Cai D.
+
+Hypothalamic stem cells control ageing speed partly through exosomal miRNAs.
+
+[[!pmid 28746310 desc="Treatement with exosomes partially compensates the loss of exosome-secreting cells, which causes accelerated ageing."]]

Ref
diff --git a/biblio/25417166.mdwn b/biblio/25417166.mdwn
new file mode 100644
index 0000000..3fba336
--- /dev/null
+++ b/biblio/25417166.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Toehold switches: de-novo-designed regulators of gene expression."]]
+[[!tag not_read synthethic_biology]]
+
+Cell. 2014 Nov 6;159(4):925-39. doi:10.1016/j.cell.2014.10.002
+
+Green AA, Silver PA, Collins JJ, Yin P.
+
+Toehold switches: de-novo-designed regulators of gene expression.
+
+[[!pmid 25417166 desc="Toehold switches are similar to conventional riboregulators, except that they have less sequence constraints, allowing for larger-scale multiplexing."]]

k-fé
diff --git a/biblio/28483779.mdwn b/biblio/28483779.mdwn
new file mode 100644
index 0000000..f87a3d8
--- /dev/null
+++ b/biblio/28483779.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Heritable L1 retrotransposition in the mouse primordial germline and early embryo."]]
+[[!tag mouse repeat]]
+
+Genome Res. 2017 May 8. doi:10.1101/gr.219022.116
+
+Richardson SR, Gerdes P, Gerhardt DJ, Sanchez-Luque FJ, Bodea GO, Muñoz-Lopez M, Jesuadian JS, Kempen MHC, Carreira PE, Jeddeloh JA, Garcia-Perez JL, Kazazian HH Jr, Ewing AD, Faulkner GJ.
+
+Heritable L1 retrotransposition in the mouse primordial germline and early embryo.
+
+[[!pmid 28483779 desc="One de novo insertion per eight mice. L1 or SINE retrotransposition may also happen in the primordial germ cells."]]

Cleanup.
diff --git a/biblio/15047890.mdwn b/biblio/15047890.mdwn
new file mode 100644
index 0000000..9a59fb3
--- /dev/null
+++ b/biblio/15047890.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Gene discovery in genetically labeled single dopaminergic neurons of the retina."]]
+[[!tag single_cell library]]
+
+Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5069-74. doi:10.1073/pnas.0400913101
+
+Gustincich S, Contini M, Gariboldi M, Puopolo M, Kadota K, Bono H, LeMieux J, Walsh P, Carninci P, Hayashizaki Y, Okazaki Y, Raviola E.
+
+Gene discovery in genetically labeled single dopaminergic neurons of the retina.
+
+[[!pmid 15047890 desc="Uses SMART7, PCR and multiple aRNA rounds."]]
diff --git a/biblio/7760832.mdwn b/biblio/7760832.mdwn
new file mode 100644
index 0000000..5001116
--- /dev/null
+++ b/biblio/7760832.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture)."]]
+[[!tag cap libraries]]
+
+Mol Cell Biol. 1995 Jun;15(6):3363-71.
+
+Edery I, Chu LL, Sonenberg N, Pelletier J.
+
+An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture).
+
+[[!pmid desc="An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 45debae..3280279 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -64,9 +64,6 @@ Uses T3N9 random nonamer amplification rounds (up to 5), after an initial T7dT R
 10499525 [single cell]
 Single cell RT-PCR.
 
-15047890 [single cell]
-Uses SMART7, PCR, and multiple aRNA rounds.
-
 12711698 [amplification] [tracked]
 Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones).
 
@@ -797,9 +794,6 @@ The cleaved DNA molecules are selected by filling of their ends with biotin-dUTP
 15156154 [emulsion]
 Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs.
 
-7760832 [libraries]
-An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length.
-
 16308152 [amplification]
 Detailed protocol for Terminal Continuation (TC).
 

Cleanup.
diff --git a/biblio/16204192.mdwn b/biblio/16204192.mdwn
new file mode 100644
index 0000000..349971e
--- /dev/null
+++ b/biblio/16204192.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels."]]
+[[!tag single_cell]]
+
+Genome Res. 2005 Oct;15(10):1388-92.
+
+Bengtsson M, Ståhlberg A, Rorsman P, Kubista M.
+
+Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels.
+
+[[!pmid 16204192 desc="Geometric mean can be more relevant than arithmetic mean when the expression follows a lognormal distribution."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 4bd092a..45debae 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -722,9 +722,6 @@ When a group of TFs have more than one Transfac entry, only the most over-repres
 11842121 [mining]
 Normalisation between print tips.
 
-16204192 [single cells]
-Geometric mean can be more relevant than arithmetic mean when the expression follows a lognormal distribution.
-
 16141248 [networks]
 Primary paper for the GeneNT R library. Clustering using direct and shortest-path distances.
 

Featured article in NAR.
diff --git a/biblio/28645155.mdwn b/biblio/28645155.mdwn
new file mode 100644
index 0000000..c123374
--- /dev/null
+++ b/biblio/28645155.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Features of genomic organization in a nucleotide-resolution molecular model of the Escherichia coli chromosome."]]
+[[!tag prokaryote chromatin not_read]]
+
+Nucleic Acids Res. 2017 Jun 22. doi:10.1093/nar/gkx541
+
+Hacker WC, Li S, Elcock AH.
+
+Features of genomic organization in a nucleotide-resolution molecular model of the Escherichia coli chromosome.
+
+[[!pmid 28645155 desc="“The chromosomal models have features similar to those of a fractal globule.”"]]

Normalise tags.
diff --git a/biblio/24053768.mdwn b/biblio/24053768.mdwn
index 0d15310..afcf8d9 100644
--- a/biblio/24053768.mdwn
+++ b/biblio/24053768.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="3' terminal diversity of MRP RNA and other human noncoding RNAs revealed by deep sequencing."]]
-[[!tag Deep-RACE sRNA]]
+[[!tag RACE sRNA]]
 
 Goldfarb KC, Cech TR
 

Add tag.
diff --git a/biblio/16629394.mdwn b/biblio/16629394.mdwn
index 1c097d5..407c97e 100644
--- a/biblio/16629394.mdwn
+++ b/biblio/16629394.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells."]]
-[[!tag RACE]]
+[[!tag single_cell RACE]]
 
 Biotechniques. 2006 Apr;40(4):469-70, 472, 474
 

Café
diff --git a/biblio/21483721.mdwn b/biblio/21483721.mdwn
new file mode 100644
index 0000000..40571a4
--- /dev/null
+++ b/biblio/21483721.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms."]]
+[[!tag method microscopy]]
+
+PLoS Biol. 2011 Apr;9(4):e1001041. doi:10.1371/journal.pbio.1001041.
+
+Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY.
+
+A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms.
+
+[[!pmid 21483721 desc="miniSOG is a fluorescent protein that liberates singlet oxygen to polymerise diaminobenzidine (DAB), a contrast agent for electron microscopy."]]

Café.
diff --git a/biblio/28751582.mdwn b/biblio/28751582.mdwn
new file mode 100644
index 0000000..58a2e12
--- /dev/null
+++ b/biblio/28751582.mdwn
@@ -0,0 +1,16 @@
+[[!meta title="ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells."]]
+[[!tag method chromatin microscopy]]
+
+Science. 2017 Jul 28;357(6349). pii: eaag0025. doi:10.1126/science.aag0025.
+
+Ou HD, Phan S, Deerinck TJ, Thor A, Ellisman MH, O'Shea CC.
+
+ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells.
+
+[[!pmid 28751582 desc="The membrane-permeable anthraquinone dye DRAQ5 (646/697
+nm) was identified by a screen for its capacity to produce singlet oxygen,
+which polymerises diaminobenzidine (DAB), which binds the electron-dense osmium
+tetroxide (OsO4).  Use of this stain suggests that there are 30 nm fibers may
+be rare in vivo or an artefact of chromatin purification.  The authors suggest
+that the absence of higher-order fibers helps compaction, because disordered
+chromatin chains have shorter persistence length."]]

Add missing PMID.
diff --git a/biblio/28673998.mdwn b/biblio/28673998.mdwn
index f2ca67d..23c4daf 100644
--- a/biblio/28673998.mdwn
+++ b/biblio/28673998.mdwn
@@ -7,4 +7,4 @@ Zhang W, Tam CP, Walton T, Fahrenbach AC, Birrane G, Szostak JW.
 
 Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.
 
-[[!pmid desc="« The formation of two Watson–Crick base pairs leads to a much higher affinity of GpppG than GMP for a CC template (Kd of ∼0.2 mM vs. ∼20 mM, respectively). » « The tight binding of GpppG to a CC template suggests that GpppG might be an ideal primer for the initiation of template copying by primer extension. The resulting RNAs would begin with a 5ʹ cap-like GpppG moiety, suggesting a potential evolutionary origin for the eukaryotic mRNA 5ʹ-cap structure. »"]]
+[[!pmid 28673998 desc="« The formation of two Watson–Crick base pairs leads to a much higher affinity of GpppG than GMP for a CC template (Kd of ∼0.2 mM vs. ∼20 mM, respectively). » « The tight binding of GpppG to a CC template suggests that GpppG might be an ideal primer for the initiation of template copying by primer extension. The resulting RNAs would begin with a 5ʹ cap-like GpppG moiety, suggesting a potential evolutionary origin for the eukaryotic mRNA 5ʹ-cap structure. »"]]

Normalise tags.
diff --git a/biblio/22761851.mdwn b/biblio/22761851.mdwn
index 80ad52a..93fad2e 100644
--- a/biblio/22761851.mdwn
+++ b/biblio/22761851.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Non-random integration of the HPV genome in cervical cancer."]]
-[[!tag HPV RACE-PCR]]
+[[!tag HPV RACE]]
 
 PLoS One. 2012;7(6):e39632. doi:10.1371/journal.pone.0039632
 

Long time ago.
diff --git a/biblio/16629394.mdwn b/biblio/16629394.mdwn
new file mode 100644
index 0000000..1c097d5
--- /dev/null
+++ b/biblio/16629394.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells."]]
+[[!tag RACE]]
+
+Biotechniques. 2006 Apr;40(4):469-70, 472, 474
+
+Ozawa T, Kishi H, Muraguchi A.
+
+Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells.
+
+[[!pmid 16629394 desc="50 % success on B cells to amplify the Ig variable region with polyG tailing and nested PCR."]]
diff --git a/biblio/To_Do b/biblio/To_Do
index 0219090..4bd092a 100644
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -932,9 +932,6 @@ Concentrations as high as 1 M or 2.5 M can strongly improve the yield.
 16533910 [enhancers]
 The directionality of the CNEs is often conserved beween paralogous loci.
 
-16629394 [single cells]
-50 % success on B cells to amplify the Ig variable region with polyG tailing and nested PCR.
-
 16682450 [misc]
 The structure of the genetic code favours secondary structures in coding RNA.
 

Biblio.
diff --git a/biblio/28673998.mdwn b/biblio/28673998.mdwn
new file mode 100644
index 0000000..f2ca67d
--- /dev/null
+++ b/biblio/28673998.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex."]]
+[[!tag cap]]
+
+Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7659-7664. doi:10.1073/pnas.1704006114
+
+Zhang W, Tam CP, Walton T, Fahrenbach AC, Birrane G, Szostak JW. 
+
+Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.
+
+[[!pmid desc="« The formation of two Watson–Crick base pairs leads to a much higher affinity of GpppG than GMP for a CC template (Kd of ∼0.2 mM vs. ∼20 mM, respectively). » « The tight binding of GpppG to a CC template suggests that GpppG might be an ideal primer for the initiation of template copying by primer extension. The resulting RNAs would begin with a 5ʹ cap-like GpppG moiety, suggesting a potential evolutionary origin for the eukaryotic mRNA 5ʹ-cap structure. »"]]

creating tag page tags/information
diff --git a/tags/information.mdwn b/tags/information.mdwn
new file mode 100644
index 0000000..ac0ae29
--- /dev/null
+++ b/tags/information.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged information"]]
+
+[[!inline pages="tagged(information)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/CRISPR
diff --git a/tags/CRISPR.mdwn b/tags/CRISPR.mdwn
new file mode 100644
index 0000000..322aa4a
--- /dev/null
+++ b/tags/CRISPR.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged CRISPR"]]
+
+[[!inline pages="tagged(CRISPR)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/storage
diff --git a/tags/storage.mdwn b/tags/storage.mdwn
new file mode 100644
index 0000000..fe9311a
--- /dev/null
+++ b/tags/storage.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged storage"]]
+
+[[!inline pages="tagged(storage)" actions="no" archive="yes"
+feedshow=10]]

Café.
diff --git a/biblio/28700573.mdwn b/biblio/28700573.mdwn
new file mode 100644
index 0000000..69a86d0
--- /dev/null
+++ b/biblio/28700573.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="CRISPR-Cas encoding of a digital movie into the genomes of a population of living bacteria."]]
+[[!tag method information storage biotechnology CRISPR]]
+
+Nature. 2017 Jul 20;547(7663):345-349. doi:10.1038/nature23017
+
+Shipman SL, Nivala J, Macklis JD, Church GM.
+
+CRISPR-Cas encoding of a digital movie into the genomes of a population of living bacteria.
+
+[[!pmid 28700573 desc="Frame order encoded by sequential transfections."]]

creating tag page tags/__40__TL__41__DNA
diff --git a/tags/__40__TL__41__DNA.mdwn b/tags/__40__TL__41__DNA.mdwn
new file mode 100644
index 0000000..819a0a6
--- /dev/null
+++ b/tags/__40__TL__41__DNA.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged (TL)DNA"]]
+
+[[!inline pages="tagged(__40__TL__41__DNA)" actions="no" archive="yes"
+feedshow=10]]

jet lag
diff --git a/biblio/18656947.mdwn b/biblio/18656947.mdwn
new file mode 100644
index 0000000..a6aa4cc
--- /dev/null
+++ b/biblio/18656947.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Triazole-linked analogue of deoxyribonucleic acid ((TL)DNA): design, synthesis, and double-strand formation with natural DNA."]]
+[[!tag (TL)DNA not_read]]
+
+Org Lett. 2008 Sep 4;10(17):3729-32. doi: 10.1021/ol801230k. Epub 2008 Jul 26.
+
+Isobe H, Fujino T, Yamazaki N, Guillot-Nieckowski M, Nakamura E.
+
+Triazole-linked analogue of deoxyribonucleic acid ((TL)DNA): design, synthesis, and double-strand formation with natural DNA.
+
+[[!pmid 18656947 desc="Synthesized using click chemistry.  (TL)DNA-DNA duplexes are more stable than DNA-DNA ones."]]

Café.
diff --git a/biblio/28655330.mdwn b/biblio/28655330.mdwn
new file mode 100644
index 0000000..701c3f1
--- /dev/null
+++ b/biblio/28655330.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="NicE-seq: high resolution open chromatin profiling."]]
+[[!tag method chromatin epigenetic]]
+
+Genome Biol. 2017 Jun 28;18(1):122. doi:10.1186/s13059-017-1247-6
+
+V. K. Chaithanya Ponnaluri, Guoqiang Zhang, Pierre-Olivier Estève, George Spracklin, Stephanie Sian, Shuang-yong Xu, Touati Benoukraf and Sriharsa Pradhan
+
+NicE-seq: high resolution open chromatin profiling.
+
+[[!pmid 28655330 desc="Uses Nt-CviPII nickase.  Extra peaks observed in fixed cells."]]

creating tag page tags/2Ome
diff --git a/tags/2Ome.mdwn b/tags/2Ome.mdwn
new file mode 100644
index 0000000..8fb6348
--- /dev/null
+++ b/tags/2Ome.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged 2Ome"]]
+
+[[!inline pages="tagged(2Ome)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/modified_base
diff --git a/tags/modified_base.mdwn b/tags/modified_base.mdwn
new file mode 100644
index 0000000..8b39112
--- /dev/null
+++ b/tags/modified_base.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged modified base"]]
+
+[[!inline pages="tagged(modified_base)" actions="no" archive="yes"
+feedshow=10]]

Café.
diff --git a/biblio/28504680.mdwn b/biblio/28504680.mdwn
new file mode 100644
index 0000000..e08327d
--- /dev/null
+++ b/biblio/28504680.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Nm-seq maps 2'-O-methylation sites in human mRNA with base precision."]]
+[[!tag method modified_base 2Ome]]
+
+Nat Methods. 2017 Jul;14(7):695-698. doi:10.1038/nmeth.4294
+
+Dai Q, Moshitch-Moshkovitz S, Han D, Kol N, Amariglio N, Rechavi G, Dominissini D, He C.
+
+Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.
+
+[[!pmid 28504680 desc=""]]

creating tag page tags/outreach
diff --git a/tags/outreach.mdwn b/tags/outreach.mdwn
new file mode 100644
index 0000000..890e2c7
--- /dev/null
+++ b/tags/outreach.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged outreach"]]
+
+[[!inline pages="tagged(outreach)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/education
diff --git a/tags/education.mdwn b/tags/education.mdwn
new file mode 100644
index 0000000..6c901cd
--- /dev/null
+++ b/tags/education.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged education"]]
+
+[[!inline pages="tagged(education)" actions="no" archive="yes"
+feedshow=10]]

Café
diff --git a/biblio/28591116.mdwn b/biblio/28591116.mdwn
new file mode 100644
index 0000000..63014f4
--- /dev/null
+++ b/biblio/28591116.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="A synthetic biology approach to integrative high school STEM training."]]
+[[!tag open_science education outreach]]
+
+Nat Biotechnol. 2017 Jun 7;35(6):591-595. doi:10.1038/nbt.3896
+
+Dubé S, Orr D, Dempsey B, Wieden HJ.
+
+A synthetic biology approach to integrative high school STEM training.
+
+[[!pmid 28591116 desc="The iGEM competition for high schools."]]

creating tag page tags/AI
diff --git a/tags/AI.mdwn b/tags/AI.mdwn
new file mode 100644
index 0000000..04b3aff
--- /dev/null
+++ b/tags/AI.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged AI"]]
+
+[[!inline pages="tagged(AI)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/open_science
diff --git a/tags/open_science.mdwn b/tags/open_science.mdwn
new file mode 100644
index 0000000..34a2ce6
--- /dev/null
+++ b/tags/open_science.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged open science"]]
+
+[[!inline pages="tagged(open_science)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/automation
diff --git a/tags/automation.mdwn b/tags/automation.mdwn
new file mode 100644
index 0000000..1cda6a7
--- /dev/null
+++ b/tags/automation.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged automation"]]
+
+[[!inline pages="tagged(automation)" actions="no" archive="yes"
+feedshow=10]]

Café
diff --git a/biblio/AI_37_2642.mdwn b/biblio/AI_37_2642.mdwn
new file mode 100644
index 0000000..3df228f
--- /dev/null
+++ b/biblio/AI_37_2642.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Artificial Intelligence to Win the Nobel Prize and Beyond: Creating the Engine for Scientific Discovery."]]
+[[!tag AI open_science automation]]
+
+AI Magazine, Spring 2016, Vol 37, No 1, pp 39–49
+
+Hiroaki Kitano
+
+Artificial Intelligence to Win the Nobel Prize and Beyond: Creating the Engine for Scientific Discovery
+
+[[“Deep phenotyping”, “combinatorial hypothesis generation”, “advanced intelligence”.|https://www.aaai.org/ojs/index.php/aimagazine/article/view/2642]]

Markdown.
diff --git a/biblio/28495877.mdwn b/biblio/28495877.mdwn
index cd31bc8..8c1b62b 100644
--- a/biblio/28495877.mdwn
+++ b/biblio/28495877.mdwn
@@ -1,4 +1,4 @@
-[[!meta title="A maternal-effect selfish genetic element in <i>Caenorhabditis elegans</i>."]]
+[[!meta title="A maternal-effect selfish genetic element in Caenorhabditis elegans."]]
 [[!tag genetics development]]
 
 Science. 2017 Jun 9;356(6342):1051-1055. doi:10.1126/science.aan0621

Markup.
diff --git a/biblio/28495877.mdwn b/biblio/28495877.mdwn
index 560c9d0..cd31bc8 100644
--- a/biblio/28495877.mdwn
+++ b/biblio/28495877.mdwn
@@ -1,4 +1,4 @@
-[[!meta title="A maternal-effect selfish genetic element in _Caenorhabditis elegans_."]]
+[[!meta title="A maternal-effect selfish genetic element in <i>Caenorhabditis elegans</i>."]]
 [[!tag genetics development]]
 
 Science. 2017 Jun 9;356(6342):1051-1055. doi:10.1126/science.aan0621

Dans le train.
diff --git a/biblio/28495877.mdwn b/biblio/28495877.mdwn
new file mode 100644
index 0000000..560c9d0
--- /dev/null
+++ b/biblio/28495877.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="A maternal-effect selfish genetic element in _Caenorhabditis elegans_."]]
+[[!tag genetics development]]
+
+Science. 2017 Jun 9;356(6342):1051-1055. doi:10.1126/science.aan0621
+
+Ben-David E, Burga A, Kruglyak L.
+
+A maternal-effect selfish genetic element in _Caenorhabditis elegans_.
+
+[[!pmid 28495877 desc="The sup-35 gene is a paralog of rmd-2 that hijacks developmental regulators to terminate embryogenesis.  It is inhibited by pha-1, which was thought to be an essential developmental regulator by itself, but is dispensable in the absence of sup-35."]]

Consolidate.
diff --git a/biblio/28495919.mdwn b/biblio/28495919.mdwn
index 56f8552..1df5507 100644
--- a/biblio/28495919.mdwn
+++ b/biblio/28495919.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Alternative TSSs are co-regulated in single cells in the mouse brain."]]
-[[!tag single_cell STRT alternative_promoter]]
+[[!tag single_cell STRT promoter]]
 
 Mol Syst Biol. 2017 May 11;13(5):930. doi:10.15252/msb.20167374.
 

creating tag page tags/STRT
diff --git a/tags/STRT.mdwn b/tags/STRT.mdwn
new file mode 100644
index 0000000..40f47b3
--- /dev/null
+++ b/tags/STRT.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged STRT"]]
+
+[[!inline pages="tagged(STRT)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/alternative_promoter
diff --git a/tags/alternative_promoter.mdwn b/tags/alternative_promoter.mdwn
new file mode 100644
index 0000000..9e8434a
--- /dev/null
+++ b/tags/alternative_promoter.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged alternative promoter"]]
+
+[[!inline pages="tagged(alternative_promoter)" actions="no" archive="yes"
+feedshow=10]]

Lu
diff --git a/biblio/28495919.mdwn b/biblio/28495919.mdwn
new file mode 100644
index 0000000..56f8552
--- /dev/null
+++ b/biblio/28495919.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Alternative TSSs are co-regulated in single cells in the mouse brain."]]
+[[!tag single_cell STRT alternative_promoter]]
+
+Mol Syst Biol. 2017 May 11;13(5):930. doi:10.15252/msb.20167374.
+
+Karlsson K, Lönnerberg P, Linnarsson S.
+
+Alternative TSSs are co-regulated in single cells in the mouse brain.
+
+[[!pmid 28495919 desc="“out of the average of 26,500 detected molecules per cell, only 3,800 (14%), could be allocated to an annotated FANTOM TSS” “some genes showed extensive 3ʹ UTR expression”"]]

Markup.
diff --git a/biblio/14971053.mdwn b/biblio/14971053.mdwn
index 6c1b2f0..5947aba 100644
--- a/biblio/14971053.mdwn
+++ b/biblio/14971053.mdwn
@@ -7,4 +7,4 @@ Oliveri M, Daga A, Lunardi C, Navone R, Millo R, Puccetti A.
 
 DNase I behaves as a transcription factor which modulates Fas expression in human cells.
 
-[[!pmid 14971053 desc="“DNase I is internalized by human cells upon binding mannose 6-phosphate receptor” (CI-MPR)']]
+[[!pmid 14971053 desc="“DNase I is internalized by human cells upon binding mannose 6-phosphate receptor” (CI-MPR)"]]

Markup.
diff --git a/biblio/14971053.mdwn b/biblio/14971053.mdwn
index a180e51..6c1b2f0 100644
--- a/biblio/14971053.mdwn
+++ b/biblio/14971053.mdwn
@@ -7,4 +7,4 @@ Oliveri M, Daga A, Lunardi C, Navone R, Millo R, Puccetti A.
 
 DNase I behaves as a transcription factor which modulates Fas expression in human cells.
 
-[[!pmid 14971053 desc='"DNase I is internalized by human cells upon binding mannose 6-phosphate receptor" (CI-MPR)']]
+[[!pmid 14971053 desc="“DNase I is internalized by human cells upon binding mannose 6-phosphate receptor” (CI-MPR)']]
diff --git a/biblio/19734907.mdwn b/biblio/19734907.mdwn
index 0f7e2ce..d13f374 100644
--- a/biblio/19734907.mdwn
+++ b/biblio/19734907.mdwn
@@ -7,4 +7,4 @@ Nat Methods. 2009 Oct;6(10):745-51. doi:10.1038/nmeth.1370
 
 Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression.
 
-[[!pmid 19734907 desc="'embryos were fixed by resuspending them in −20 °C methanol (80%). The tube was then placed on an overhead rotator at 4 °C for at least 1 h.'"]]
+[[!pmid 19734907 desc="“embryos were fixed by resuspending them in −20 °C methanol (80%). The tube was then placed on an overhead rotator at 4 °C for at least 1 h”"]]
diff --git a/biblio/28526029.mdwn b/biblio/28526029.mdwn
index 99abfae..ac2005f 100644
--- a/biblio/28526029.mdwn
+++ b/biblio/28526029.mdwn
@@ -7,4 +7,4 @@ BMC Biol. 2017 May 19;15(1):44. doi:10.1186/s12915-017-0383-5
 
 Cell fixation and preservation for droplet-based single-cell transcriptomics.
 
-[[!pmid 28526029 desc='"For rehydration, cells were either kept on ice after fixation (Fixed) or moved from –80 °C to 4 °C (Fixed 1 or 3 weeks) and kept in the cold throughout the procedure. Cells were pelleted at 1000 to 3000 × g, resuspended in PBS + 0.01% BSA, centrifuged again, resuspended in PBS + 0.01% BSA, passed through a 40- or 35-μm cell strainer, counted and diluted for Drop-seq in PBS + 0.01% BSA as described above. "']]
+[[!pmid 28526029 desc="“For rehydration, cells were either kept on ice after fixation (Fixed) or moved from –80 °C to 4 °C (Fixed 1 or 3 weeks) and kept in the cold throughout the procedure. Cells were pelleted at 1000 to 3000 × g, resuspended in PBS + 0.01% BSA, centrifuged again, resuspended in PBS + 0.01% BSA, passed through a 40- or 35-μm cell strainer, counted and diluted for Drop-seq in PBS + 0.01% BSA as described above.”"]]

Markup.
diff --git a/biblio/17332513.mdwn b/biblio/17332513.mdwn
index 9f7db37..5448198 100644
--- a/biblio/17332513.mdwn
+++ b/biblio/17332513.mdwn
@@ -7,4 +7,4 @@ Panchision DM, Chen HL, Pistollato F, Papini D, Ni HT, Hawley TS.
 
 Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24.
 
-[[!pmid 17332513 desc='"On two occasions with papain and three occasions with TrypLE, we saw free-DNA aggregation even in the presence of DNase I, indicating substantial cell lysis.  We could not definitively determine the cause of this variability. Two additional papain and one TrypLE sample could not be properly assayed by hemocytometer because of DNA aggregation and so were discarded. Accutase and Liberase-1 never generated free DNA aggregates."']]
+[[!pmid 17332513 desc="“On two occasions with papain and three occasions with TrypLE, we saw free-DNA aggregation even in the presence of DNase I, indicating substantial cell lysis.  We could not definitively determine the cause of this variability. Two additional papain and one TrypLE sample could not be properly assayed by hemocytometer because of DNA aggregation and so were discarded. Accutase and Liberase-1 never generated free DNA aggregates.”"]]

Au bureau.
diff --git a/biblio/17332513.mdwn b/biblio/17332513.mdwn
new file mode 100644
index 0000000..9f7db37
--- /dev/null
+++ b/biblio/17332513.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24."]]
+[[!tag DNAse_I method]]
+
+Stem Cells. 2007 Jun;25(6):1560-70. Epub 2007 Mar 1.
+
+Panchision DM, Chen HL, Pistollato F, Papini D, Ni HT, Hawley TS.
+
+Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24.
+
+[[!pmid 17332513 desc='"On two occasions with papain and three occasions with TrypLE, we saw free-DNA aggregation even in the presence of DNase I, indicating substantial cell lysis.  We could not definitively determine the cause of this variability. Two additional papain and one TrypLE sample could not be properly assayed by hemocytometer because of DNA aggregation and so were discarded. Accutase and Liberase-1 never generated free DNA aggregates."']]
diff --git a/biblio/28526029.mdwn b/biblio/28526029.mdwn
index 08b22ae..99abfae 100644
--- a/biblio/28526029.mdwn
+++ b/biblio/28526029.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Cell fixation and preservation for droplet-based single-cell transcriptomics."]]
-[[!tag methanol fixation method single_cell]]
+[[!tag methanol fixation method single_cell DNAse_I]]
 
 Alles J, Karaiskos N, Praktiknjo SD, Grosswendt S, Wahle P, Ruffault PL, Ayoub S, Schreyer L, Boltengagen A, Birchmeier C, Zinzen R, Kocks C, Rajewsky N.
 

creating tag page tags/DNAse_I
diff --git a/tags/DNAse_I.mdwn b/tags/DNAse_I.mdwn
new file mode 100644
index 0000000..27dcce6
--- /dev/null
+++ b/tags/DNAse_I.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged DNAse I"]]
+
+[[!inline pages="tagged(DNAse_I)" actions="no" archive="yes"
+feedshow=10]]

Au bureau.
diff --git a/biblio/14971053.mdwn b/biblio/14971053.mdwn
new file mode 100644
index 0000000..a180e51
--- /dev/null
+++ b/biblio/14971053.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="DNase I behaves as a transcription factor which modulates Fas expression in human cells."]]
+[[!tag DNAse_I method]]
+
+Eur J Immunol. 2004 Jan;34(1):273-9.
+
+Oliveri M, Daga A, Lunardi C, Navone R, Millo R, Puccetti A.
+
+DNase I behaves as a transcription factor which modulates Fas expression in human cells.
+
+[[!pmid 14971053 desc='"DNase I is internalized by human cells upon binding mannose 6-phosphate receptor" (CI-MPR)']]

Au bureau.
diff --git a/biblio/28526029.mdwn b/biblio/28526029.mdwn
new file mode 100644
index 0000000..08b22ae
--- /dev/null
+++ b/biblio/28526029.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Cell fixation and preservation for droplet-based single-cell transcriptomics."]]
+[[!tag methanol fixation method single_cell]]
+
+Alles J, Karaiskos N, Praktiknjo SD, Grosswendt S, Wahle P, Ruffault PL, Ayoub S, Schreyer L, Boltengagen A, Birchmeier C, Zinzen R, Kocks C, Rajewsky N.
+
+BMC Biol. 2017 May 19;15(1):44. doi:10.1186/s12915-017-0383-5
+
+Cell fixation and preservation for droplet-based single-cell transcriptomics.
+
+[[!pmid 28526029 desc='"For rehydration, cells were either kept on ice after fixation (Fixed) or moved from –80 °C to 4 °C (Fixed 1 or 3 weeks) and kept in the cold throughout the procedure. Cells were pelleted at 1000 to 3000 × g, resuspended in PBS + 0.01% BSA, centrifuged again, resuspended in PBS + 0.01% BSA, passed through a 40- or 35-μm cell strainer, counted and diluted for Drop-seq in PBS + 0.01% BSA as described above. "']]

Mastodon.
diff --git "a/r\303\251seauxsociaux.txt" "b/r\303\251seauxsociaux.txt"
index 1ad370b..05b094a 100644
--- "a/r\303\251seauxsociaux.txt"
+++ "b/r\303\251seauxsociaux.txt"
@@ -7,21 +7,21 @@ https:://copainsdavant.linternaute.com/membre/520697/7398950458/charles_plessy/
 https://github.com/charles-plessy
 https://www.biostars.org/u/13132/
 https://publons.com/author/793793
-
+https://framapiaf.org/@charles_plessy
+https://mastodon.technology/@charles_plessy
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Biblio.
diff --git a/biblio/19734907.mdwn b/biblio/19734907.mdwn
new file mode 100644
index 0000000..0f7e2ce
--- /dev/null
+++ b/biblio/19734907.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression."]]
+[[!tag methanol fixation not_read miRNA]]
+
+Stoeckius M, Maaskola J, Colombo T, Rahn HP, Friedländer MR, Li N, Chen W, Piano F, Rajewsky N.
+
+Nat Methods. 2009 Oct;6(10):745-51. doi:10.1038/nmeth.1370
+
+Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression.
+
+[[!pmid 19734907 desc="'embryos were fixed by resuspending them in −20 °C methanol (80%). The tube was then placed on an overhead rotator at 4 °C for at least 1 h.'"]]

Sans café.
diff --git a/biblio/28473583.mdwn b/biblio/28473583.mdwn
new file mode 100644
index 0000000..dad5bf0
--- /dev/null
+++ b/biblio/28473583.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells."]]
+[[!tag CpG epigenetic promoter]]
+
+Science. 2017 May 5;356(6337):503-508. doi:10.1126/science.aag3260
+
+Takahashi Y, Wu J, Suzuki K, Martinez-Redondo P, Li M, Liao HK, Wu MZ, Hernández-Benítez R, Hishida T, Shokhirev MN, Esteban CR, Sancho-Martinez I, Belmonte JCI.
+
+Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells.
+
+[[!pmid 28473583 desc="“both interruption of the methylation-blocking machinery and re- pression of transcriptional activity are necessary and sufficient to trigger de novo CGI methylation in PSCs”"]]

creating tag page tags/methanol
diff --git a/tags/methanol.mdwn b/tags/methanol.mdwn
new file mode 100644
index 0000000..94c022e
--- /dev/null
+++ b/tags/methanol.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged methanol"]]
+
+[[!inline pages="tagged(methanol)" actions="no" archive="yes"
+feedshow=10]]

jc
diff --git a/biblio/10.1101-104844.mdwn b/biblio/10.1101-104844.mdwn
index 678aa70..608ffba 100644
--- a/biblio/10.1101-104844.mdwn
+++ b/biblio/10.1101-104844.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Comprehensive single cell transcriptional profiling of a multicellular organism by combinatorial indexing"]]
-[[!tag single_cell transcriptome methanol fixation method]]
+[[!tag single_cell transcriptome methanol fixation method journal_club]]
 
 bioRxiv, posted February 2, 2017.
 

Journal club.
diff --git a/biblio/10.1101-104844.mdwn b/biblio/10.1101-104844.mdwn
new file mode 100644
index 0000000..678aa70
--- /dev/null
+++ b/biblio/10.1101-104844.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Comprehensive single cell transcriptional profiling of a multicellular organism by combinatorial indexing"]]
+[[!tag single_cell transcriptome methanol fixation method]]
+
+bioRxiv, posted February 2, 2017.
+
+Junyue Cao, Jonathan S. Packer, Vijay Ramani, Darren A. Cusanovich, Chau Huynh, Riza Daza, Xiaojie Qiu, Choli Lee, Scott N. Furlan, Frank J. Steemers, Andrew Adey, Robert H. Waterston, Cole Trapnell, Jay Shendure
+
+Comprehensive single cell transcriptional profiling of a multicellular organism by combinatorial indexing
+
+[[!doi 10.1101/104844 desc="Methanol fixation."]]

Petite correction.
diff --git a/Japon/kanji/abeille.mdwn b/Japon/kanji/abeille.mdwn
index ab7a7b1..ba07127 100644
--- a/Japon/kanji/abeille.mdwn
+++ b/Japon/kanji/abeille.mdwn
@@ -1,7 +1,7 @@
 [[!meta title="蜂 – hatchi, l'abeille, la guêpe, ..."]]
 [[!meta date="2014-12-31"]]
 
-<span title="hatchi, l'abeille, la guêpe, ..." id="abeille" class="kanji">[[!unihan 蜂]]</span> – l'abeille, la guêpe, ...
+<span title="hatchi, l'abeille, la guêpe, ..." id="abeille" class="kanji">[[!unihan 蜂]]</span> – hatchi, l'abeille, la guêpe, ...
 
 10 mai 2017 (水)
 
@@ -16,4 +16,4 @@ l'entraînement, on ne se roule ni dans les fleurs ni dans le miel !
 
 En japonais, abeille se dit « mitsupatchi » (蜜蜂), l'abeille à miel.  Et miel
 se dit « hatchimitsu » (蜂蜜), le miel d'abeille.  Attention à ne pas
-confondre.  Vous reprendrez bien une tartine de pain beurée aux abeilles ?
+confondre.  Vous reprendrez bien une tartine de pain beurrée aux abeilles ?

Le miel et les abeilles.
diff --git a/Japon/kanji/abeille.mdwn b/Japon/kanji/abeille.mdwn
new file mode 100644
index 0000000..ab7a7b1
--- /dev/null
+++ b/Japon/kanji/abeille.mdwn
@@ -0,0 +1,19 @@
+[[!meta title="蜂 – hatchi, l'abeille, la guêpe, ..."]]
+[[!meta date="2014-12-31"]]
+
+<span title="hatchi, l'abeille, la guêpe, ..." id="abeille" class="kanji">[[!unihan 蜂]]</span> – l'abeille, la guêpe, ...
+
+10 mai 2017 (水)
+
+J'ai investi dans l'aïkidô beaucoup du temps libre que j'ai récupéré quand
+Simon a grandi.  Plusieurs entraînements par semaine, et maintenant que le
+froids de l'hiver s'en est allé pour de bon mon dôgui (on ne dit pas kimono)
+sèche plus rapidement sur le balcon.  J'ai du le secouer très fort pour en
+faire partir une abeille qui visiblement le trouvait très attirant.  Pourtant à
+l'entraînement, on ne se roule ni dans les fleurs ni dans le miel !
+
+[[!img images/abeille.jpg alt="abeille" title="Bzzz" link="none"]]
+
+En japonais, abeille se dit « mitsupatchi » (蜜蜂), l'abeille à miel.  Et miel
+se dit « hatchimitsu » (蜂蜜), le miel d'abeille.  Attention à ne pas
+confondre.  Vous reprendrez bien une tartine de pain beurée aux abeilles ?
diff --git a/Japon/kanji/images/abeille.jpg b/Japon/kanji/images/abeille.jpg
new file mode 100644
index 0000000..4a5630f
Binary files /dev/null and b/Japon/kanji/images/abeille.jpg differ

Signet.
diff --git a/biblio/28473589.mdwn b/biblio/28473589.mdwn
new file mode 100644
index 0000000..255a913
--- /dev/null
+++ b/biblio/28473589.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Negative selection in humans and fruit flies involves synergistic epistasis."]]
+[[!tag genetics not_read]]
+
+Science. 2017 May 5;356(6337):539-542. doi:10.1126/science.aah5238
+
+Sohail M, Vakhrusheva OA, Sul JH, Pulit SL, Francioli LC; Genome of the Netherlands Consortium; Alzheimer’s Disease Neuroimaging Initiative, van den Berg LH, Veldink JH, de Bakker PIW, Bazykin GA, Kondrashov AS, Sunyaev SR.
+
+Negative selection in humans and fruit flies involves synergistic epistasis.
+
+[[!pmid 28473589 desc="'Deleterious LOF mutations are further away from each other in the genome than expected by chance."]]

creating tag page tags/selex
diff --git a/tags/selex.mdwn b/tags/selex.mdwn
new file mode 100644
index 0000000..3a2d146
--- /dev/null
+++ b/tags/selex.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged selex"]]
+
+[[!inline pages="tagged(selex)" actions="no" archive="yes"
+feedshow=10]]

Café.
diff --git a/biblio/28473536.mdwn b/biblio/28473536.mdwn
new file mode 100644
index 0000000..81755ea
--- /dev/null
+++ b/biblio/28473536.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Impact of cytosine methylation on DNA binding specificities of human transcription factors."]]
+[[!tag mC selex transcription_factor epigenetic]]
+
+Yin Y, Morgunova E, Jolma A, Kaasinen E, Sahu B, Khund-Sayeed S, Das PK, Kivioja T, Dave K, Zhong F, Nitta KR, Taipale M, Popov A, Ginno PA, Domcke S, Yan J, Schübeler D, Vinson C, Taipale J.
+
+Science. 2017 May 5;356(6337). pii: eaaj2239. doi:10.1126/science.aaj2239
+
+Impact of cytosine methylation on DNA binding specificities of human transcription factors.
+
+[[!pmid 28473536 desc="“Binding of most major classes of transcription factors, including bHLH, bZIP, and ETS, was inhibited by mCpG. In contrast, transcription factors such as homeodomain, POU, and NFAT proteins preferred to bind methylated DNA.”"]]

Correction des couleurs.
diff --git a/trombine/mon_colors.png b/trombine/mon_colors.png
index fefb891..e7998a0 100644
Binary files a/trombine/mon_colors.png and b/trombine/mon_colors.png differ
diff --git a/trombine/mon_colors.svg b/trombine/mon_colors.svg
index 2fd1fde..f285246 100644
--- a/trombine/mon_colors.svg
+++ b/trombine/mon_colors.svg
@@ -9,15 +9,16 @@
    xmlns="http://www.w3.org/2000/svg"
    xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd"
    xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape"
-   width="440.65701"
-   height="440.65701"
+   width="470.03415"
+   height="470.03415"
    id="svg2"
    version="1.1"
-   inkscape:version="0.91 r13725"
+   inkscape:version="0.92.1 r15371"
    sodipodi:docname="mon_colors.svg"
-   inkscape:export-filename="/home/charles/sites/charles-plessy-org.branchable.com/trombine/mon_colors.png"
+   inkscape:export-filename="/home/charles/sites/charles-plessy-org/source/trombine/mon_colors.png"
    inkscape:export-xdpi="90.069801"
-   inkscape:export-ydpi="90.069801">
+   inkscape:export-ydpi="90.069801"
+   viewBox="0 0 440.65701 440.65701">
   <defs
      id="defs4" />
   <sodipodi:namedview
@@ -27,9 +28,9 @@
      borderopacity="1.0"
      inkscape:pageopacity="0.0"
      inkscape:pageshadow="2"
-     inkscape:zoom="0.44329156"
-     inkscape:cx="-63.472836"
-     inkscape:cy="-96.388738"
+     inkscape:zoom="0.31345447"
+     inkscape:cx="447.86373"
+     inkscape:cy="-26.848655"
      inkscape:document-units="px"
      inkscape:current-layer="layer1"
      showgrid="false"
@@ -37,9 +38,9 @@
      showguides="true"
      inkscape:guide-bbox="true"
      inkscape:window-width="1279"
-     inkscape:window-height="1369"
-     inkscape:window-x="1279"
-     inkscape:window-y="30"
+     inkscape:window-height="1016"
+     inkscape:window-x="641"
+     inkscape:window-y="27"
      inkscape:window-maximized="0"
      fit-margin-top="0"
      fit-margin-left="0"
@@ -51,7 +52,11 @@
        empspacing="5"
        visible="true"
        enabled="true"
-       snapvisiblegridlinesonly="true" />
+       snapvisiblegridlinesonly="true"
+       originx="0"
+       originy="0"
+       spacingx="1"
+       spacingy="1" />
   </sodipodi:namedview>
   <metadata
      id="metadata7">
@@ -70,28 +75,26 @@
      inkscape:groupmode="layer"
      id="layer1"
      transform="translate(-33.297594,-589.29391)">
+    <ellipse
+       transform="matrix(0.53239866,0.53239866,-0.53214988,0.53214988,278.37177,450.23091)"
+       style="fill:none;stroke:#2aa198;stroke-width:47.0715332;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
+       id="path3910"
+       inkscape:export-xdpi="90.069786"
+       inkscape:export-ydpi="90.069786"
+       cx="314.28571"
+       cy="360.93362"
+       rx="265.71429"
+       ry="272.85715" />
     <path
        sodipodi:nodetypes="ccc"
        inkscape:connector-curvature="0"
        id="path3908"
        d="m 254.84585,654.74341 153.6638,153.6639 -156.1031,156.10314"
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-    <path
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-       sodipodi:cx="314.28571"
-       sodipodi:cy="360.93362"
-       sodipodi:rx="265.71429"
-       sodipodi:ry="272.85715"
-       d="M 580,360.93362 A 265.71429,272.85715 0 0 1 314.28571,633.79077 265.71429,272.85715 0 0 1 48.571411,360.93362 265.71429,272.85715 0 0 1 314.28571,88.076477 265.71429,272.85715 0 0 1 580,360.93362 Z"
+       style="fill:none;stroke:#cb4b16;stroke-width:35.43307114;stroke-linecap:square;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        inkscape:export-xdpi="90.069786"
        inkscape:export-ydpi="90.069786" />
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-       style="fill:none;stroke:#268bd2;stroke-width:35.43307114;stroke-linecap:square;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-opacity:1;stroke-dasharray:none"
+       style="fill:none;stroke:#268bd2;stroke-width:35.43307114;stroke-linecap:square;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        d="m 323.42605,815.84711 -70.499,70.499 -69.1846,-69.1846"
        id="path3912"
        inkscape:connector-curvature="0"
@@ -99,7 +102,7 @@
        inkscape:export-xdpi="90.069786"
        inkscape:export-ydpi="90.069786" />
     <path
-       style="fill:none;stroke:#d33682;stroke-width:35.43307114;stroke-linecap:square;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-opacity:1;stroke-dasharray:none"
+       style="fill:none;stroke:#d33682;stroke-width:35.43307114;stroke-linecap:square;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        d="M 200.71835,912.82221 98.742717,810.84651 201.63045,707.95871"
        id="path3914"
        inkscape:connector-curvature="0"
@@ -107,7 +110,7 @@
        inkscape:export-xdpi="90.069786"
        inkscape:export-ydpi="90.069786" />
     <path
-       style="fill:none;stroke:#002b36;stroke-width:35.43307114;stroke-linecap:butt;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-opacity:1;stroke-dasharray:none"
+       style="fill:none;stroke:#859900;stroke-width:35.43307114;stroke-linecap:butt;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        d="m 261.22055,825.03991 -57.0638,-57.0637"
        id="path3916"
        inkscape:connector-curvature="0"
@@ -117,7 +120,7 @@
     <path
        id="path3918"
        d="m 238.53205,733.60101 57.0637,57.0637"
-       style="fill:#000000;stroke:#859900;stroke-width:35.43307114;stroke-linecap:butt;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-opacity:1;stroke-dasharray:none;fill-opacity:0"
+       style="fill:#000000;fill-opacity:0;stroke:#859900;stroke-width:35.43307114;stroke-linecap:butt;stroke-linejoin:bevel;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
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        inkscape:export-xdpi="90.069786"

Un long café.
diff --git a/biblio/28160599.mdwn b/biblio/28160599.mdwn
new file mode 100644
index 0000000..84162c0
--- /dev/null
+++ b/biblio/28160599.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase."]]
+[[!tag enzyme lariat]]
+
+Döring J, Hurek T.
+
+Nucleic Acids Res. 2017 Apr 20;45(7):3967-3984. doi: 10.1093/nar/gkx073.
+
+Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase.
+
+[[!pmid 28160599 desc="Despite it is only single-stranded, the 2′ arm can be
+cleaved when polymerisation is primed on the 3′ arm, but not the converse."]]

En diagonale.
diff --git a/biblio/28397782.mdwn b/biblio/28397782.mdwn
new file mode 100644
index 0000000..091c71b
--- /dev/null
+++ b/biblio/28397782.mdwn
@@ -0,0 +1,13 @@
+[[!meta title="Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice."]]
+[[!tag HPV cancer]]
+
+Zhong Z, Zhai Y, Bu P, Shah S, Qiao L.
+
+Nat Commun. 2017 Apr 11;8:15004. doi:10.1038/ncomms15004.
+
+Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice.
+
+[[!pmid 28397782 desc="HPV pseudoviruses have tropsim for cancer cells (other
+cells in this system do not express the needed heparan sulfate proteoglycains
+in the needed conformation).  Capsid and (non-replicative plasmid) DNA activate
+innate immunity against the infected cancer cells."]]

Publié!
diff --git a/biblio/28349422.mdwn b/biblio/28349422.mdwn
new file mode 100644
index 0000000..75c473b
--- /dev/null
+++ b/biblio/28349422.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="NanoCAGE: A Method for the Analysis of Coding and Noncoding 5'-Capped Transcriptomes"]]
+[[!tag nanoCAGE]]
+
+Poulain S, Kato S, Arnaud O, Morlighem JÉ, Suzuki M, Plessy C, Harbers M.
+
+Methods Mol Biol. 2017;1543:57-109. doi:10.1007/978-1-4939-6716-2_4
+
+NanoCAGE: A Method for the Analysis of Coding and Noncoding 5'-Capped Transcriptomes
+
+[[!pmid 28349422 desc="Replaces Library PCR with tagmentation."]]

Café.
diff --git a/biblio/28263961.mdwn b/biblio/28263961.mdwn
new file mode 100644
index 0000000..e4dd327
--- /dev/null
+++ b/biblio/28263961.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Power analysis of single-cell RNA-sequencing experiments."]]
+[[!tag single_cell spike]]
+
+Svensson V, Natarajan KN, Ly LH, Miragaia RJ, Labalette C, Macaulay IC, Cvejic A, Teichmann SA.
+
+Nat Methods. 2017 Apr;14(4):381-387. doi:10.1038/nmeth.4220
+
+Power analysis of single-cell RNA-sequencing experiments.
+
+[[!pmid 28263961 desc="Freeze-thaw cycles decrease detected amounts of ERCC spike molecules."]]

La semaine dernière.
diff --git a/biblio/28265063.mdwn b/biblio/28265063.mdwn
new file mode 100644
index 0000000..8b5306e
--- /dev/null
+++ b/biblio/28265063.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers."]]
+[[!tag enzyme]]
+
+Zhu B, Wang L, Mitsunobu H, Lu X, Hernandez AJ, Yoshida-Takashima Y, Nunoura T, Tabor S, Richardson CC.
+
+Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):E2310-E2318. doi:10.1073/pnas.1700280114
+
+Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers.
+
+[[!pmid 28265063 desc="'This gene product has efficient DNA polymerase activity and is processive in DNA synthesis in the presence of the NrS-1 helicase and ssDNA-binding protein.' 'The optimal reaction temperature for de novo DNA synthesis by NrS-1 DNA polymerase is 50 °C.' 'The most efficient sites for de novo initiation of DNA by NrS-1 DNA polymerase are 5′-TTTGGTCA-3′ and 5′-TTTGGTTA-3′'"]]

Ce soir.
diff --git a/biblio/24852250.mdwn b/biblio/24852250.mdwn
new file mode 100644
index 0000000..9b2acf9
--- /dev/null
+++ b/biblio/24852250.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="De novo detection of differentially bound regions for ChIP-seq data using peaks and windows: controlling error rates correctly."]]
+[[!tag method peak statistics]]
+
+Nucleic Acids Res. 2014 Jun;42(11):e95. doi:10.1093/nar/gku351
+
+Lun AT, Smyth GK.
+
+De novo detection of differentially bound regions for ChIP-seq data using peaks and windows: controlling error rates correctly.
+
+[[!pmid 24852250 desc="'Peak calling should be performed on pooled libraries prior to the statistical analysis.'"]]

Quotes.
diff --git a/biblio/28225755.mdwn b/biblio/28225755.mdwn
index d7a4c61..af08693 100644
--- a/biblio/28225755.mdwn
+++ b/biblio/28225755.mdwn
@@ -7,4 +7,4 @@ Neri F, Rapelli S, Krepelova A, Incarnato D, Parlato C, Basile G, Maldotti M, An
 
 Intragenic DNA methylation prevents spurious transcription initiation.
 
-[[!pmid 28225755 desc='"Dnmt3b knockout increases intragenic RNA Pol II spurious entry and transcription initiation events on gene body." Uses DECAP-seq']]
+[[!pmid 28225755 desc="'Dnmt3b knockout increases intragenic RNA Pol II spurious entry and transcription initiation events on gene body.' Uses DECAP-seq"]]

En diagonale.
diff --git a/biblio/26201400.mdwn b/biblio/26201400.mdwn
new file mode 100644
index 0000000..cefdb31
--- /dev/null
+++ b/biblio/26201400.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos."]]
+[[!tag single_cell random_priming method]]
+
+Genome Biol. 2015 Jul 23;16:148. doi:10.1186/s13059-015-0706-1
+
+Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang F, Huang Y.
+
+Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos.
+
+[[!pmid 26201400 desc="'Single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq).' Linkers added by poly-a tailing of first-strand cDNAs.  Random primers but very low levels of rRNA reads."]]

Solved !
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index afd3354..bc40c41 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2017-03-04 13:05+0000\n"
-"PO-Revision-Date: 2017-02-10 13:48+0900\n"
+"PO-Revision-Date: 2017-03-04 22:06+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -22,10 +22,9 @@ msgid "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 msgstr "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
-#, fuzzy, no-wrap
-#| msgid "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
+#, no-wrap
 msgid "[[!meta updated=\"Sat, 04 Mar 2017 22:04:36 +0900\"]]\n"
-msgstr "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
+msgstr "[[!meta updated=\"Sat, 04 Mar 2017 22:04:36 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
@@ -33,10 +32,8 @@ msgid "[[!tag Debian]]\n"
 msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
-#, fuzzy
-#| msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
 msgid "[[!meta title=\"[résolu] Attention à libinput 1.6.0-1\"]]"
-msgstr "[[!meta title=\"Beware of libinput 1.6.0-1\"]]\n"
+msgstr "[[!meta title=\"[solved] Beware of libinput 1.6.0-1\"]]"
 
 #. type: Plain text
 msgid ""
@@ -65,3 +62,5 @@ msgid ""
 "***m-à-j :*** Les paquets version 1.6.2-1, encore dans Sid pour le\n"
 "moment, fonctionnent parfaitement.  Merci !\n"
 msgstr ""
+"***update:*** The packages version\n"
+"[1.6.1-1] in Sid work perfectly. Thanks!\n"

updated PO files
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index cbe3ba3..afd3354 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -6,7 +6,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2017-02-10 04:48+0000\n"
+"POT-Creation-Date: 2017-03-04 13:05+0000\n"
 "PO-Revision-Date: 2017-02-10 13:48+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
@@ -22,8 +22,9 @@ msgid "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 msgstr "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
-#, no-wrap
-msgid "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
+#, fuzzy, no-wrap
+#| msgid "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
+msgid "[[!meta updated=\"Sat, 04 Mar 2017 22:04:36 +0900\"]]\n"
 msgstr "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
 
 #. type: Plain text
@@ -32,8 +33,9 @@ msgid "[[!tag Debian]]\n"
 msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
-#, no-wrap
-msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
+#, fuzzy
+#| msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
+msgid "[[!meta title=\"[résolu] Attention à libinput 1.6.0-1\"]]"
 msgstr "[[!meta title=\"Beware of libinput 1.6.0-1\"]]\n"
 
 #. type: Plain text
@@ -56,3 +58,10 @@ msgstr ""
 "***update:*** To reinstall packages version\n"
 "[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) solves the \n"
 "problem in the meantime.\n"
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"***m-à-j :*** Les paquets version 1.6.2-1, encore dans Sid pour le\n"
+"moment, fonctionnent parfaitement.  Merci !\n"
+msgstr ""

Problème résolu.
diff --git "a/Debian/debi\303\242neries/libinput.mdwn" "b/Debian/debi\303\242neries/libinput.mdwn"
index e1d6266..38b4a32 100644
--- "a/Debian/debi\303\242neries/libinput.mdwn"
+++ "b/Debian/debi\303\242neries/libinput.mdwn"
@@ -1,8 +1,8 @@
 [[!meta date="Thu, 09 Feb 2017 22:22:11 +0900"]]
-[[!meta updated="Fri, 10 Feb 2017 13:47:06 +0900"]]
+[[!meta updated="Sat, 04 Mar 2017 22:04:36 +0900"]]
 [[!tag Debian]]
 
-[[!meta title="Attention à libinput 1.6.0-1"]]
+[[!meta title="[résolu] Attention à libinput 1.6.0-1"]]
 
 Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus cliquer en
 tapotant mon pavé tactile.  Heureusement, [une correction est en
@@ -11,3 +11,6 @@ route](https://bugs.debian.org/853869).
 ***m-à-j :*** Réinstaller les paquets version
 [1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résout le
 problème en attendant.
+
+***m-à-j :*** Les paquets version 1.6.2-1, encore dans Sid pour le
+moment, fonctionnent parfaitement.  Merci !

creating tag page tags/H3K36me3
diff --git a/tags/H3K36me3.mdwn b/tags/H3K36me3.mdwn
new file mode 100644
index 0000000..c22c3f7
--- /dev/null
+++ b/tags/H3K36me3.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged H3K36me3"]]
+
+[[!inline pages="tagged(H3K36me3)" actions="no" archive="yes"
+feedshow=10]]

Ce matin.
diff --git a/biblio/28225755.mdwn b/biblio/28225755.mdwn
new file mode 100644
index 0000000..d7a4c61
--- /dev/null
+++ b/biblio/28225755.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Intragenic DNA methylation prevents spurious transcription initiation."]]
+[[!tag exon_painting CpG methylation H3K36me3]]
+
+Nature. 2017 Feb 22. doi:10.1038/nature21373
+
+Neri F, Rapelli S, Krepelova A, Incarnato D, Parlato C, Basile G, Maldotti M, Anselmi F, Oliviero S.
+
+Intragenic DNA methylation prevents spurious transcription initiation.
+
+[[!pmid 28225755 desc='"Dnmt3b knockout increases intragenic RNA Pol II spurious entry and transcription initiation events on gene body." Uses DECAP-seq']]

creating tag page tags/systems_biology
diff --git a/tags/systems_biology.mdwn b/tags/systems_biology.mdwn
new file mode 100644
index 0000000..8e15e77
--- /dev/null
+++ b/tags/systems_biology.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged systems biology"]]
+
+[[!inline pages="tagged(systems_biology)" actions="no" archive="yes"
+feedshow=10]]

En diagonale.
diff --git a/biblio/28193641.mdwn b/biblio/28193641.mdwn
new file mode 100644
index 0000000..ab9284c
--- /dev/null
+++ b/biblio/28193641.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="A method for high-throughput production of sequence-verified DNA libraries and strain collections."]]
+[[!tag library yeast barcode systems_biology]]
+
+Mol Syst Biol. 2017 Feb 13;13(2):913. doi:10.15252/msb.20167233.
+
+Smith JD, Schlecht U, Xu W, Suresh S, Horecka J, Proctor MJ, Aiyar RS, Bennett RA, Chu A, Li YF, Roy K, Davis RW, Steinmetz LM, Hyman RW, Levy SF, St Onge RP.
+
+A method for high-throughput production of sequence-verified DNA libraries and strain collections.
+
+[[!pmid 28193641 desc="Clone a library of array-synthesised oligonucleotides in halpoid yeast, mate them to a library of barcoded haploid yeast, recombine, sequence the recombinants in bulk, and select the haploids that had an oligonucleotide without synthesis error."]]

Lu à l'écran.
diff --git a/biblio/28202002.mdwn b/biblio/28202002.mdwn
new file mode 100644
index 0000000..a6fee1f
--- /dev/null
+++ b/biblio/28202002.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16."]]
+[[!tag HPV]]
+
+BMC Cancer. 2017 Feb 15;17(1):136. doi: 10.1186/s12885-017-3124-9.
+
+Hong D, Liu J, Hu Y, Lu X, Li B, Li Y, Hu D, Lu W, Xie X, Cheng X.
+
+Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16.
+
+[[!pmid 28202002 desc='"In 93 samples, 21.5% (20/93) presented purely integrated viral genome, 53.8% (50/93) mixed viral genome, and 24.7% (23/93) purely episomal viral genome."']]

creating tag page tags/Trizol
diff --git a/tags/Trizol.mdwn b/tags/Trizol.mdwn
new file mode 100644
index 0000000..f8261c2
--- /dev/null
+++ b/tags/Trizol.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged Trizol"]]
+
+[[!inline pages="tagged(Trizol)" actions="no" archive="yes"
+feedshow=10]]

Lu dans le lit.
diff --git a/biblio/22749402.mdwn b/biblio/22749402.mdwn
new file mode 100644
index 0000000..4239e79
--- /dev/null
+++ b/biblio/22749402.mdwn
@@ -0,0 +1,13 @@
+[[!meta title="Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells."]]
+[[!tag purification method Trizol miRNA bias]]
+
+Mol Cell. 2012 Jun 29;46(6):893-5. doi:10.1016/j.molcel.2012.05.036
+
+Kim YK, Yeo J, Kim B, Ha M, Kim VN.
+
+Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells.
+
+[[!pmid 22749402 desc="In extraction methods using Trizol, the long RNAs present in the cell lysate
+act as carrier for the short RNAs, and when initial cell numbers are low or magnesium ions
+are chelated, structured small RNAs become selectively harder to capture, which causes
+a bias that can be confounded with key experimental parameters."]]

creating tag page tags/ATAC-seq
diff --git a/tags/ATAC-seq.mdwn b/tags/ATAC-seq.mdwn
new file mode 100644
index 0000000..dcd7b18
--- /dev/null
+++ b/tags/ATAC-seq.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged ATAC-seq"]]
+
+[[!inline pages="tagged(ATAC-seq)" actions="no" archive="yes"
+feedshow=10]]

Lu dans l'aérogare de Jeddah.
diff --git a/biblio/26083756.mdwn b/biblio/26083756.mdwn
new file mode 100644
index 0000000..f9a34f8
--- /dev/null
+++ b/biblio/26083756.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Single-cell chromatin accessibility reveals principles of regulatory variation."]]
+[[!tag single-cell ATAC-seq tag]]
+
+Nature. 2015 Jul 23;523(7561):486-90. doi:10.1038/nature14590
+
+Buenrostro JD, Wu B, Litzenburger UM, Ruff D, Gonzales ML, Snyder MP, Chang HY, Greenleaf WJ.
+
+Single-cell chromatin accessibility reveals principles of regulatory variation.
+
+[[!pmid 26083756 desc="Because the data is sparse, peaks with similar properties need to be analysed as pools."]]

Engrish.
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index 430a279..cbe3ba3 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2017-02-10 04:48+0000\n"
-"PO-Revision-Date: 2017-02-10 13:45+0900\n"
+"PO-Revision-Date: 2017-02-10 13:48+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -22,10 +22,9 @@ msgid "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 msgstr "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
-#, fuzzy, no-wrap
-#| msgid "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
+#, no-wrap
 msgid "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
-msgstr "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
+msgstr "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
@@ -38,11 +37,6 @@ msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
 msgstr "[[!meta title=\"Beware of libinput 1.6.0-1\"]]\n"
 
 #. type: Plain text
-#, fuzzy
-#| msgid ""
-#| "Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en "
-#| "tapotant mon pavé tactile.  Heureusement, [une correction est en route]"
-#| "(https://bugs.debian.org/853869)."
 msgid ""
 "Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus cliquer en "
 "tapotant mon pavé tactile.  Heureusement, [une correction est en route]"
@@ -53,16 +47,12 @@ msgstr ""
 "org/853869)."
 
 #. type: Plain text
-#, fuzzy, no-wrap
-#| msgid ""
-#| "***m-à-j*** Réinstaller les paquets version\n"
-#| "[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le\n"
-#| "problème en attendant.\n"
+#, no-wrap
 msgid ""
 "***m-à-j :*** Réinstaller les paquets version\n"
 "[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résout le\n"
 "problème en attendant.\n"
 msgstr ""
 "***update:*** To reinstall packages version\n"
-"[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) solved the \n"
+"[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) solves the \n"
 "problem in the meantime.\n"

updated PO files
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index e26eec8..430a279 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -6,7 +6,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2017-02-10 04:45+0000\n"
+"POT-Creation-Date: 2017-02-10 04:48+0000\n"
 "PO-Revision-Date: 2017-02-10 13:45+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
@@ -22,8 +22,9 @@ msgid "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 msgstr "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
-#, no-wrap
-msgid "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
+#, fuzzy, no-wrap
+#| msgid "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
+msgid "[[!meta updated=\"Fri, 10 Feb 2017 13:47:06 +0900\"]]\n"
 msgstr "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
@@ -37,8 +38,13 @@ msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
 msgstr "[[!meta title=\"Beware of libinput 1.6.0-1\"]]\n"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en "
+#| "tapotant mon pavé tactile.  Heureusement, [une correction est en route]"
+#| "(https://bugs.debian.org/853869)."
 msgid ""
-"Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en "
+"Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus cliquer en "
 "tapotant mon pavé tactile.  Heureusement, [une correction est en route]"
 "(https://bugs.debian.org/853869)."
 msgstr ""
@@ -54,7 +60,7 @@ msgstr ""
 #| "problème en attendant.\n"
 msgid ""
 "***m-à-j :*** Réinstaller les paquets version\n"
-"[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le\n"
+"[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résout le\n"
 "problème en attendant.\n"
 msgstr ""
 "***update:*** To reinstall packages version\n"

Lissage.
diff --git "a/Debian/debi\303\242neries/libinput.mdwn" "b/Debian/debi\303\242neries/libinput.mdwn"
index 8426787..e1d6266 100644
--- "a/Debian/debi\303\242neries/libinput.mdwn"
+++ "b/Debian/debi\303\242neries/libinput.mdwn"
@@ -1,13 +1,13 @@
 [[!meta date="Thu, 09 Feb 2017 22:22:11 +0900"]]
-[[!meta updated="Thu, 09 Feb 2017 22:22:11 +0900"]]
+[[!meta updated="Fri, 10 Feb 2017 13:47:06 +0900"]]
 [[!tag Debian]]
 
 [[!meta title="Attention à libinput 1.6.0-1"]]
 
-Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en
+Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus cliquer en
 tapotant mon pavé tactile.  Heureusement, [une correction est en
 route](https://bugs.debian.org/853869).
 
 ***m-à-j :*** Réinstaller les paquets version
-[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le
+[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résout le
 problème en attendant.

updated PO files
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index 8423428..e26eec8 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -6,7 +6,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2017-02-10 04:44+0000\n"
+"POT-Creation-Date: 2017-02-10 04:45+0000\n"
 "PO-Revision-Date: 2017-02-10 13:45+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
@@ -47,9 +47,13 @@ msgstr ""
 "org/853869)."
 
 #. type: Plain text
-#, no-wrap
+#, fuzzy, no-wrap
+#| msgid ""
+#| "***m-à-j*** Réinstaller les paquets version\n"
+#| "[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le\n"
+#| "problème en attendant.\n"
 msgid ""
-"***m-à-j*** Réinstaller les paquets version\n"
+"***m-à-j :*** Réinstaller les paquets version\n"
 "[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le\n"
 "problème en attendant.\n"
 msgstr ""

Typographie.
diff --git "a/Debian/debi\303\242neries/libinput.mdwn" "b/Debian/debi\303\242neries/libinput.mdwn"
index 868b020..8426787 100644
--- "a/Debian/debi\303\242neries/libinput.mdwn"
+++ "b/Debian/debi\303\242neries/libinput.mdwn"
@@ -8,6 +8,6 @@ Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en
 tapotant mon pavé tactile.  Heureusement, [une correction est en
 route](https://bugs.debian.org/853869).
 
-***m-à-j*** Réinstaller les paquets version
+***m-à-j :*** Réinstaller les paquets version
 [1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le
 problème en attendant.

En English.
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index e57b046..8423428 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2017-02-10 04:44+0000\n"
-"PO-Revision-Date: 2017-02-09 22:30+0900\n"
+"PO-Revision-Date: 2017-02-10 13:45+0900\n"
 "Last-Translator: Charles Plessy <toto@example.com>\n"
 "Language-Team: \n"
 "Language: en\n"
@@ -53,3 +53,6 @@ msgid ""
 "[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le\n"
 "problème en attendant.\n"
 msgstr ""
+"***update:*** To reinstall packages version\n"
+"[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) solved the \n"
+"problem in the meantime.\n"

updated PO files
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index 662f7d8..e57b046 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -6,14 +6,14 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2017-02-09 13:27+0000\n"
+"POT-Creation-Date: 2017-02-10 04:44+0000\n"
 "PO-Revision-Date: 2017-02-09 22:30+0900\n"
+"Last-Translator: Charles Plessy <toto@example.com>\n"
+"Language-Team: \n"
 "Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
-"Last-Translator: Charles Plessy <toto@example.com>\n"
-"Language-Team: \n"
 "X-Generator: Poedit 1.8.11\n"
 
 #. type: Plain text
@@ -43,5 +43,13 @@ msgid ""
 "(https://bugs.debian.org/853869)."
 msgstr ""
 "Since I updated this evening, touch to click with my touchpad is almost "
-"totally broken.  Fortunately, [a correction is pending](https://bugs."
-"debian.org/853869)."
+"totally broken.  Fortunately, [a correction is pending](https://bugs.debian."
+"org/853869)."
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"***m-à-j*** Réinstaller les paquets version\n"
+"[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le\n"
+"problème en attendant.\n"
+msgstr ""

Downgrade.
diff --git "a/Debian/debi\303\242neries/libinput.mdwn" "b/Debian/debi\303\242neries/libinput.mdwn"
index 1cb2d89..868b020 100644
--- "a/Debian/debi\303\242neries/libinput.mdwn"
+++ "b/Debian/debi\303\242neries/libinput.mdwn"
@@ -7,3 +7,7 @@
 Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en
 tapotant mon pavé tactile.  Heureusement, [une correction est en
 route](https://bugs.debian.org/853869).
+
+***m-à-j*** Réinstaller les paquets version
+[1.5.5-4](http://snapshot.debian.org/package/libinput/1.5.4-1/) résoud le
+problème en attendant.

Translated.
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
index 9a39611..662f7d8 100644
--- "a/Debian/debi\303\242neries/libinput.en.po"
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -3,42 +3,45 @@
 # This file is distributed under the same license as the PACKAGE package.
 # FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
 #
-#, fuzzy
 msgid ""
 msgstr ""
-"Project-Id-Version: PACKAGE VERSION\n"
+"Project-Id-Version: \n"
 "POT-Creation-Date: 2017-02-09 13:27+0000\n"
-"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
-"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
-"Language-Team: LANGUAGE <LL@li.org>\n"
-"Language: \n"
+"PO-Revision-Date: 2017-02-09 22:30+0900\n"
+"Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
+"Last-Translator: Charles Plessy <toto@example.com>\n"
+"Language-Team: \n"
+"X-Generator: Poedit 1.8.11\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!tag Debian]]\n"
-msgstr ""
+msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
-msgstr ""
+msgstr "[[!meta title=\"Beware of libinput 1.6.0-1\"]]\n"
 
 #. type: Plain text
 msgid ""
 "Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en "
-"tapotant mon pavé tactile.  Heureusement, [une correction est en "
-"route](https://bugs.debian.org/853869)."
+"tapotant mon pavé tactile.  Heureusement, [une correction est en route]"
+"(https://bugs.debian.org/853869)."
 msgstr ""
+"Since I updated this evening, touch to click with my touchpad is almost "
+"totally broken.  Fortunately, [a correction is pending](https://bugs."
+"debian.org/853869)."

updated PO files
diff --git "a/Debian/debi\303\242neries/libinput.en.po" "b/Debian/debi\303\242neries/libinput.en.po"
new file mode 100644
index 0000000..9a39611
--- /dev/null
+++ "b/Debian/debi\303\242neries/libinput.en.po"
@@ -0,0 +1,44 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2017-02-09 13:27+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Thu, 09 Feb 2017 22:22:11 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Attention à libinput 1.6.0-1\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en "
+"tapotant mon pavé tactile.  Heureusement, [une correction est en "
+"route](https://bugs.debian.org/853869)."
+msgstr ""

Touché, coulé.
diff --git "a/Debian/debi\303\242neries/libinput.mdwn" "b/Debian/debi\303\242neries/libinput.mdwn"
new file mode 100644
index 0000000..1cb2d89
--- /dev/null
+++ "b/Debian/debi\303\242neries/libinput.mdwn"
@@ -0,0 +1,9 @@
+[[!meta date="Thu, 09 Feb 2017 22:22:11 +0900"]]
+[[!meta updated="Thu, 09 Feb 2017 22:22:11 +0900"]]
+[[!tag Debian]]
+
+[[!meta title="Attention à libinput 1.6.0-1"]]
+
+Depuis que j'ai mis à jour ce soir, je ne peux quasiment plus clicker en
+tapotant mon pavé tactile.  Heureusement, [une correction est en
+route](https://bugs.debian.org/853869).

Avec un gros rhume.
diff --git a/biblio/28108474.mdwn b/biblio/28108474.mdwn
new file mode 100644
index 0000000..f56b9a2
--- /dev/null
+++ b/biblio/28108474.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters."]]
+[[!tag human promoter motif]]
+
+Genes Dev. 2017 Jan 1;31(1):6-11. doi:10.1101/gad.293837.116
+
+Vo Ngoc L, Cassidy CJ, Huang CY, Duttke SH, Kadonaga JT.
+
+The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters.
+
+[[!pmid 28108474 desc="A BBCABW motif, stricter than just YR.  But 'focused core promoters' do not cover all kinds of TSSes."]]

TOC du matin.
diff --git a/biblio/28002401.mdwn b/biblio/28002401.mdwn
new file mode 100644
index 0000000..2d998a8
--- /dev/null
+++ b/biblio/28002401.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Reversible methylation of m6Am in the 5' cap controls mRNA stability."]]
+[[!tag not_read cap]]
+
+Nature. 2016 Dec 21. doi:10.1038/nature21022
+
+Mauer J, Luo X, Blanjoie A, Jiao X, Grozhik AV, Patil DP, Linder B, Pickering BF, Vasseur JJ, Chen Q, Gross SS, Elemento O, Debart F, Kiledjian M, Jaffrey SR.
+
+Reversible methylation of m6Am in the 5' cap controls mRNA stability.
+
+[[!pmid 28002401 desc="Functional role for mAm cap in mRNAs."]]

Lu dans le train.
diff --git a/biblio/27317694.mdwn b/biblio/27317694.mdwn
new file mode 100644
index 0000000..52c2a74
--- /dev/null
+++ b/biblio/27317694.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="mRNA capping: biological functions and applications."]]
+[[!tag review cap]]
+
+Nucleic Acids Res. 2016 Sep 19;44(16):7511-26. doi:10.1093/nar/gkw551
+
+Ramanathan A, Robb GB, Chan SH
+
+mRNA capping: biological functions and applications.
+
+[[!pmid 27317694 desc="Written by NEB researchers."]]

Lu en partie.
diff --git a/biblio/25326703.mdwn b/biblio/25326703.mdwn
new file mode 100644
index 0000000..9d255e7
--- /dev/null
+++ b/biblio/25326703.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing."]]
+[[!tag method enzyme transposase]]
+
+Nat Genet. 2014 Dec;46(12):1343-9. doi:10.1038/ng.3119
+
+Amini S, Pushkarev D, Christiansen L, Kostem E, Royce T, Turk C, Pignatelli N,
+Adey A, Kitzman JO, Vijayan K, Ronaghi M, Shendure J, Gunderson KL, Steemers FJ.
+
+Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing.
+
+[[!pmid 25326703 desc="The Tn5 transposase still binds DNA after ligating its payload."]]

Normalisation.
diff --git a/biblio/27353563.mdwn b/biblio/27353563.mdwn
index 6801263..fe707b6 100644
--- a/biblio/27353563.mdwn
+++ b/biblio/27353563.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Droplet barcoding for massively parallel single-molecule deep sequencing."]]
-[[!tag emulsion microfluidic barcode transposase single_molecule]]
+[[!tag method emulsion microfluidic barcode transposase single_molecule]]
 
 Nat Commun. 2016 Jun 29;7:11784. doi: 10.1038/ncomms11784.