Dernières modifications :

Nigon elements
diff --git a/biblio/30770412.mdwn b/biblio/30770412.mdwn
new file mode 100644
index 00000000..5fe1c8e5
--- /dev/null
+++ b/biblio/30770412.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Chromosome-Wide Evolution and Sex Determination in the Three-Sexed Nematode Auanema rhodensis."]]
+[[!tag nematode]]
+
+Tandonnet S, Koutsovoulos GD, Adams S, Cloarec D, Parihar M, Blaxter ML, Pires-daSilva A.
+
+Chromosome-Wide Evolution and Sex Determination in the Three-Sexed Nematode Auanema rhodensis.
+
+G3 (Bethesda). 2019 Apr 9;9(4):1211-1230. doi: 10.1534/g3.119.0011
+
+[[!pmid 30770412 desc="Nigon elements"]]
diff --git a/tags/nematode.mdwn b/tags/nematode.mdwn
index d79694c9..626da130 100644
--- a/tags/nematode.mdwn
+++ b/tags/nematode.mdwn
@@ -2,6 +2,9 @@
 
 ... in progress ...
 
+Nematode chromosomes have been called “Nigon elements” by [[Tandonnet and coll,
+2019|biblio/30770412]].
+
 ### _C. inopinata_
 
  - _C. inopinata_ was discovered in 2018 to be the closest species to _C.

Nematodes
diff --git a/biblio/31007946.mdwn b/biblio/31007946.mdwn
new file mode 100644
index 00000000..62c4cfcb
--- /dev/null
+++ b/biblio/31007946.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Comparative genomics of 10 new _Caenorhabditis_ species."]]
+[[!tag nematode]]
+
+Stevens L, Félix MA, Beltran T, Braendle C, Caurcel C, Fausett S, Fitch D, Frézal L, Gosse C, Kaur T, Kiontke K, Newton MD, Noble LM, Richaud A, Rockman MV, Sudhaus W, Blaxter M.
+
+Evol Lett. 2019 Apr 2;3(2):217-236. doi:10.1002/evl3.110
+
+Comparative genomics of 10 new _Caenorhabditis_ species.
+
+[[!pmid 31007946 desc="“Phylogenetic relationships of 32 Caenorhabditis species”"]]
diff --git a/tags/nematode.mdwn b/tags/nematode.mdwn
index 001fd69c..d79694c9 100644
--- a/tags/nematode.mdwn
+++ b/tags/nematode.mdwn
@@ -29,6 +29,10 @@
 
 ### Other nematodes
 
+ - A phylogeny of 32 _Caenorhabditis_ species shows [ (_briggsae_ | _nigoni_) |
+   (_latens_ | _remanei_) ] | (_elegans_ | _inopinata_) ([[Stevens and coll.,
+   2019|biblio/31007946]]).
+
  - _Diploscapter pachys_ has a single chromosome, that is still organised in
    ancestral domains homologous to the ones of other nematodes ([[Fradin and
    coll., 2019|biblio/28943090]]).

rm refs
diff --git a/biblio/37726270.mdwn b/biblio/37726270.mdwn
index d427e9ce..491b1d5c 100644
--- a/biblio/37726270.mdwn
+++ b/biblio/37726270.mdwn
@@ -7,4 +7,4 @@ Nat Commun. 2023 Sep 19;14(1):5617. doi:10.1038/s41467-023-41220-x
 
 Evolutionary origin of genomic structural variations in domestic yaks.
 
-[[!pmid 37726270 desc="“assemblies were constructed for 6 wild and 15 domestic yaks”  “we used a uniform standard pipeline to annotate these 47 bovine genomes. We identified an average of 24,368 protein-coding genes for each assembly” “1,048,639 high-confidence SNPs were detected and used in phylogenetic analyses with the water buffalo genome as outgroup”  “We further constructed a species tree on the basis of 8428 single-copy core genes through selecting one representative individual of each species”  “Pangenomes were constructed for yaks and cattle and a super-pangenome for the 7 Bovini species. For the yak pangenome the total gene set approached saturation at n = 20. The percentages of core (present in all 22 genomes), near-core (present in 20–21 genomes) and variable (found in 1–19 genomes) gene families were 50.18, 10.91, 38.91%, respectively.”  “ In pairwise comparisons of the assemblies constituting the pangenome, each assembly possessed 123 to 2113 genes not present in the other genome”   we constructed a multi-assembly graph-based genome of the 47 genomes used in the phylogenomic and super-pangenome analyses. This comprised 3.14 gigabases (Gb) spread across 5,449,222 nodes (the number of fragments of sequences) and connected by 4,889,530 edges (the connections between nodes), with non-reference nodes spanning 387.0 Mb. The core (shared by all genomes), near-core (in 46 or 45 genomes) and variable nodes (in 44 or less samples) accounted for 60.8, 17.0, 22.2% of all nodes.”  “We detected SVs ( ≥ 50 bp) in the graph-based genome using the bubble popping algorithm of gfatools25 and retained 293,712 SVs (81.7% <500 bp, 99.76% <10 kb) that could be genotyped in the BosMut3.0 yak reference genome or at least one other genome”  “Next, we used the graph-genotyping software Vg (v1.36.0)26 on 386 bovines (233 yaks, 140 cattle, 4 bison, 8 wisent and one gaur, including the novel genome sequences for assembling the super-pangenome) for which resequencing data with >6× coverage were available (Supplementary Data 1, 2)2,3,8,20,21,27,28,29,30,31,32,33,34,35,36. This yielded 610,921 genotyped SVs, from which 57,432 were retained after quality filtering”"]]
+[[!pmid 37726270 desc="“assemblies were constructed for 6 wild and 15 domestic yaks”  “we used a uniform standard pipeline to annotate these 47 bovine genomes. We identified an average of 24,368 protein-coding genes for each assembly” “1,048,639 high-confidence SNPs were detected and used in phylogenetic analyses with the water buffalo genome as outgroup”  “We further constructed a species tree on the basis of 8428 single-copy core genes through selecting one representative individual of each species”  “Pangenomes were constructed for yaks and cattle and a super-pangenome for the 7 Bovini species. For the yak pangenome the total gene set approached saturation at n = 20. The percentages of core (present in all 22 genomes), near-core (present in 20–21 genomes) and variable (found in 1–19 genomes) gene families were 50.18, 10.91, 38.91%, respectively.”  “ In pairwise comparisons of the assemblies constituting the pangenome, each assembly possessed 123 to 2113 genes not present in the other genome”   we constructed a multi-assembly graph-based genome of the 47 genomes used in the phylogenomic and super-pangenome analyses. This comprised 3.14 gigabases (Gb) spread across 5,449,222 nodes (the number of fragments of sequences) and connected by 4,889,530 edges (the connections between nodes), with non-reference nodes spanning 387.0 Mb. The core (shared by all genomes), near-core (in 46 or 45 genomes) and variable nodes (in 44 or less samples) accounted for 60.8, 17.0, 22.2% of all nodes.”  “We detected SVs ( ≥ 50 bp) in the graph-based genome using the bubble popping algorithm of gfatools25 and retained 293,712 SVs (81.7% <500 bp, 99.76% <10 kb) that could be genotyped in the BosMut3.0 yak reference genome or at least one other genome”  “Next, we used the graph-genotyping software Vg (v1.36.0) on 386 bovines (233 yaks, 140 cattle, 4 bison, 8 wisent and one gaur, including the novel genome sequences for assembling the super-pangenome) for which resequencing data with >6× coverage were available. This yielded 610,921 genotyped SVs, from which 57,432 were retained after quality filtering”"]]

Café
diff --git a/biblio/37726270.mdwn b/biblio/37726270.mdwn
new file mode 100644
index 00000000..d427e9ce
--- /dev/null
+++ b/biblio/37726270.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Evolutionary origin of genomic structural variations in domestic yaks."]]
+[[!tag variants]]
+
+Liu X, Liu W, Lenstra JA, Zheng Z, Wu X, Yang J, Li B, Yang Y, Qiu Q, Liu H, Li K, Liang C, Guo X, Ma X, Abbott RJ, Kang M, Yan P, Liu J.
+
+Nat Commun. 2023 Sep 19;14(1):5617. doi:10.1038/s41467-023-41220-x
+
+Evolutionary origin of genomic structural variations in domestic yaks.
+
+[[!pmid 37726270 desc="“assemblies were constructed for 6 wild and 15 domestic yaks”  “we used a uniform standard pipeline to annotate these 47 bovine genomes. We identified an average of 24,368 protein-coding genes for each assembly” “1,048,639 high-confidence SNPs were detected and used in phylogenetic analyses with the water buffalo genome as outgroup”  “We further constructed a species tree on the basis of 8428 single-copy core genes through selecting one representative individual of each species”  “Pangenomes were constructed for yaks and cattle and a super-pangenome for the 7 Bovini species. For the yak pangenome the total gene set approached saturation at n = 20. The percentages of core (present in all 22 genomes), near-core (present in 20–21 genomes) and variable (found in 1–19 genomes) gene families were 50.18, 10.91, 38.91%, respectively.”  “ In pairwise comparisons of the assemblies constituting the pangenome, each assembly possessed 123 to 2113 genes not present in the other genome”   we constructed a multi-assembly graph-based genome of the 47 genomes used in the phylogenomic and super-pangenome analyses. This comprised 3.14 gigabases (Gb) spread across 5,449,222 nodes (the number of fragments of sequences) and connected by 4,889,530 edges (the connections between nodes), with non-reference nodes spanning 387.0 Mb. The core (shared by all genomes), near-core (in 46 or 45 genomes) and variable nodes (in 44 or less samples) accounted for 60.8, 17.0, 22.2% of all nodes.”  “We detected SVs ( ≥ 50 bp) in the graph-based genome using the bubble popping algorithm of gfatools25 and retained 293,712 SVs (81.7% <500 bp, 99.76% <10 kb) that could be genotyped in the BosMut3.0 yak reference genome or at least one other genome”  “Next, we used the graph-genotyping software Vg (v1.36.0)26 on 386 bovines (233 yaks, 140 cattle, 4 bison, 8 wisent and one gaur, including the novel genome sequences for assembling the super-pangenome) for which resequencing data with >6× coverage were available (Supplementary Data 1, 2)2,3,8,20,21,27,28,29,30,31,32,33,34,35,36. This yielded 610,921 genotyped SVs, from which 57,432 were retained after quality filtering”"]]

WFH
diff --git a/biblio/37277269.mdwn b/biblio/37277269.mdwn
new file mode 100644
index 00000000..69875eb4
--- /dev/null
+++ b/biblio/37277269.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="Gelatinous larvacean zooplankton can enhance trophic transfer and carbon sequestration."]]
+[[!tag Oikopleura review]]
+
+Jaspers C, Hopcroft RR, Kiørboe T, Lombard F, López-Urrutia Á, Everett JD, Richardson AJ.
+
+Trends Ecol Evol. 2023 Oct;38(10):980-993. doi:10.1016/j.tree.2023.05.005
+
+Gelatinous larvacean zooplankton can enhance trophic transfer and carbon sequestration.
+
+[[!pmid 37277269 desc="Role of appendicularians in the biological pump to the
+sea floor.  “Larvacean shunt” in the food chain."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 4c1d660f..774f1cac 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -584,6 +584,8 @@ Phenotypes
 Ecology
 -------
 
+ - The ecological role of appendicularians was reviewed by [[Jaspers and coll,
+   2023|biblio/37277269]].
  - _O. dioica_ grazes on bacterioplankton, which can be a significant share
    of its own diet, but the grazing has only “minimal influence on the
    population dynamics of the free-living bacteria” ([[King, Hollibaugh and

add a tag
diff --git a/biblio/36867684.mdwn b/biblio/36867684.mdwn
index 3a99120f..9d8b2f7f 100644
--- a/biblio/36867684.mdwn
+++ b/biblio/36867684.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. "]]
-[[!tag genome clock]]
+[[!tag genome clock phylogeny]]
 
 Huang Z, Xu L, Cai C, Zhou Y, Liu J, Xu Z, Zhu Z, Kang W, Cen W, Pei S, Chen D, Shi C, Wu X, Huang Y, Xu C, Yan Y, Yang Y, Xue T, He W, Hu X, Zhang Y, Chen Y, Bi C, He C, Xue L, Xiao S, Yue Z, Jiang Y, Yu JK, Jarvis ED, Li G, Lin G, Zhang Q, Zhou Q. 
 

Café
diff --git a/biblio/37402370.mdwn b/biblio/37402370.mdwn
new file mode 100644
index 00000000..d51cc30d
--- /dev/null
+++ b/biblio/37402370.mdwn
@@ -0,0 +1,39 @@
+[[!meta title="Mechanisms of insertions at a DNA double-strand break."]]
+[[!tag variants]]
+
+Min J, Zhao J, Zagelbaum J, Lee J, Takahashi S, Cummings P, Schooley A, Dekker J, Gottesman ME, Rabadan R, Gautier J. 
+
+Mol Cell. 2023 Jul 20;83(14):2434-2448.e7. doi: 10.1016/j.molcel.2023.06.016
+
+Mechanisms of insertions at a DNA double-strand break.
+
+[[!pmid 37402370 desc="Indel-seq: double-strand breaks are induced with the
+AsiSI endonuclease or CRISPR, and a target locus is studied by targeted
+sequencing of PCR amplicons.  “the median size of inserts is 120 bp [...] 39%
+of inserts originate from repetitive sequences, including telomeres,
+centromeres, Alu, and retrotransposon elements”  “Inhibition [with NU7441] of
+DNA-PK catalytic activity [involved in NHEJ] significantly decreased insertion
+events. In contrast, the size and number of deletions increased.”  “POLQ
+inhibition did not reduce [...] insertions, indicating that insertions were
+largely independent of microhomology-mediated end joining (MMEJ)”  “Treatment
+with CK-666, a small-molecule inhibitor that stabilizes the Arp2/3 complex in
+an inactive conformation, resulted in a significant decrease in insertion
+events originating from AsiSI-proximal donor sequences”  “siRNA-mediated
+downregulation of RAD51 did not affect the overall number of insertions but
+dramatically [increased] distal (class 3) insertions”.  “8.7% of insertions
+originated from multiple genomic loci on different chromosomes”  “more than 70%
+of donor sequences originated from promoters and transcribed gene body regions”
+“Donor sequences are spread around the AsiSI sites and span the TSS; however,
+insertions preferentially originate from the transcribed side [...]. In
+contrast, donor sequences originating from AsiSI-cleaved DSBs within a gene
+body or distal intergenic regions did not show any bias”  ”[Insertions in] a
+Cas9 DSB near the TSS of the BCL6 locus in a [the OCI-Ly7 cell line] were
+markedly enriched in the direction of transcription.”  “alpha-amanitin
+decreased the frequency of insertions, preferentially those originating from
+promoter-proximal DSBs [...]. Furthermore, donor sequences in
+alpha-amanitin-treated cells were no longer preferentially derived from the
+transcribed side.” “[Upregulating transcription of telomeric repeat-containing
+RNA at telomerse via] FANCM downregulation resulted in a significant increase
+in telomeric insertions.”  “RNA-DNA hybrid disruption by RNaseH1 suppressed the
+bias of donor sequences that originate preferentially from the direction of
+transcription”"]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index c86aa2db..d23fcca7 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -83,6 +83,12 @@ the same transposon participate in an aberrant transposition event to a new
 site”.  They give an example where the two transposons are identical copies
 on sister chromatids in G2 phase.
 
+## Indels
+
+Indel-seq ([[Min and coll., 2023|biblio/37402370]]) shows insertions at
+double-strand breaks originating from donor regions possibly single-stranded by
+transcription (R-loops) or repair.  Proximity and contact appear to be important.
+
 ## Software
 
  - _NanoSV_ ([[Cretu Stancu and coll., 2017|biblio/29109544]]) uses nanopore long

Café
diff --git a/biblio/37407817.mdwn b/biblio/37407817.mdwn
new file mode 100644
index 00000000..054c1d2a
--- /dev/null
+++ b/biblio/37407817.mdwn
@@ -0,0 +1,22 @@
+[[!meta title="Hepatitis C virus RNA is 5'-capped with flavin adenine dinucleotide"]]
+[[!tag cap]]
+
+Sherwood AV, Rivera-Rangel LR, Ryberg LA, Larsen HS, Anker KM, Costa R, Vågbø CB, Jakljevič E, Pham LV, Fernandez-Antunez C, Indrisiunaite G, Podolska-Charlery A, Grothen JER, Langvad NW, Fossat N, Offersgaard A, Al-Chaer A, Nielsen L, Kuśnierczyk A, Sølund C, Weis N, Gottwein JM, Holmbeck K, Bottaro S, Ramirez S, Bukh J, Scheel TKH, Vinther J. Hepatitis C virus RNA is 5'-capped with flavin adenine dinucleotide. 
+
+Nature. 2023 Jul;619(7971):811-818. doi:10.1038/s41586-023-06301-3.
+
+Hepatitis C virus RNA is 5'-capped with flavin adenine dinucleotide.
+
+[[!pmid 37407817 desc="Arabidopsis thaliana Nudix pyrophosphohydrolase 23
+(AtNUDX23) decapping followed with adapter ligation results in libraries
+enriched in HCV(+) and HCV(-) RNAs.  75% of the HCV RNAs are capped and most of
+the rest is 5′ triphosphate.  FAD capping was not found in “bovine viral
+diarrhea virus (BVDV) and the ortho flavivirus tick-borne encephalitis virus
+(TBEV), both from the Flaviviridae family, as well as chikungunya virus
+(CHIKV), an alphavirus of the Togaviridae family“.  “no replication of
+JFH1-SGR-Feo was observed upon riboflavin depletion, whereas replication was
+rescued by supplementing exogenous riboflavin or FAD”  “inclusion of FAD as the
+priming nucleotide resulted in efficient de novo initiation of replication and
+production of an initiation product consisting of FAD linked to CMP”
+“Incubation of 5′-ppp RNA with recombinant NS5B and flavin mononucleotide (FMN)
+did not result in 5′-FAD capping”"]]
diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index 175d7267..4b5f3495 100644
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -77,6 +77,9 @@ Atypical caps (work in progress)
  - Mass spectroscopy and molecular biology detected dinucleoside polyphosphate caps
    in bacteria ([[Hudeček and coll., 2020|biblio/32103016]]).
 
+ - Hepatitis C virus RNA is 5′-capped with flavin adenine dinucleotide
+   ([[Sherwood and coll., 2023|biblio/37407817]]).
+
 Cap physiology (work in progress)
 ---------------------------------
 

Ce matin
diff --git a/biblio/32761142.mdwn b/biblio/32761142.mdwn
new file mode 100644
index 00000000..d7488c72
--- /dev/null
+++ b/biblio/32761142.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="NCBI Taxonomy: a comprehensive update on curation, resources and tools."]]
+[[!tag taxonomy database]]
+
+Schoch CL, Ciufo S, Domrachev M, Hotton CL, Kannan S, Khovanskaya R, Leipe D, Mcveigh R, O'Neill K, Robbertse B, Sharma S, Soussov V, Sullivan JP, Sun L, Turner S, Karsch-Mizrachi I.
+
+Database (Oxford). 2020 Jan 1;2020:baaa062. doi:10.1093/database/baaa062
+
+NCBI Taxonomy: a comprehensive update on curation, resources and tools.
+
+[[!pmid 32761142 desc="83% of the know invertebrate species are still not in the database."]]

creating tag page tags/priming.
diff --git a/tags/priming..mdwn b/tags/priming..mdwn
new file mode 100644
index 00000000..bee9db76
--- /dev/null
+++ b/tags/priming..mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged priming."]]
+
+[[!inline pages="tagged(priming.)" actions="no" archive="yes"
+feedshow=10]]

creating tag page tags/random
diff --git a/tags/random.mdwn b/tags/random.mdwn
new file mode 100644
index 00000000..ee7ac848
--- /dev/null
+++ b/tags/random.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged random"]]
+
+[[!inline pages="tagged(random)" actions="no" archive="yes"
+feedshow=10]]

Dans l'avion
diff --git a/biblio/28623350.mdwn b/biblio/28623350.mdwn
new file mode 100644
index 00000000..49d9f8db
--- /dev/null
+++ b/biblio/28623350.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Multiplexed Spliced-Leader Sequencing: A high-throughput, selective method for RNA-seq in Trypanosomatids."]]
+[[!tag trans-splicing method random priming.]]
+
+Cuypers B, Domagalska MA, Meysman P, Muylder G, Vanaerschot M, Imamura H, Dumetz F, Verdonckt TW, Myler PJ, Ramasamy G, Laukens K, Dujardin JC.
+
+Sci Rep. 2017 Jun 16;7(1):3725. doi:10.1038/s41598-017-03987-0
+
+Multiplexed Spliced-Leader Sequencing: A high-throughput, selective method for RNA-seq in Trypanosomatids.
+
+[[!pmidi 28623350 desc="1 µg total RNA reverse-transcribed with SuperScript III and 2 µM random primer (GTATAAGAGACAGNNNNNNN).  The RNA strand degraded with 2U RNAse H for 20 mi at 37 °C.  The DNA strand was purified with Agencourt AMPure XP beads.  0.6 mM of SL primer (TCAGTTTCTGTA) was annealed to 25 µL of DNA from the previous step in 1x NEB buffer at 98 °C for 5 minutes and cooled down to room temperature for min.  Second-strand synthesis with 5U Klenow fragment and 0.4 mM dNTPs in 50 µL at 37 °C for 60 minutes."]]
diff --git a/tags/random_priming.mdwn b/tags/random_priming.mdwn
index 8cc4d277..7a0b1d9a 100644
--- a/tags/random_priming.mdwn
+++ b/tags/random_priming.mdwn
@@ -1,4 +1,7 @@
 [[!meta title="pages tagged random priming"]]
 
-[[!inline pages="tagged(random_priming)" actions="no" archive="yes"
-feedshow=10]]
+Work in progress.
+
+ - 2 µM N7, 1 µg total RNA, SuperScript III ([[Cuypers and coll., 2017|biblio/28623350]]), 
+
+[[!inline pages="tagged(random_priming)" actions="no" limit=0]]

creating tag page tags/archaea
diff --git a/tags/archaea.mdwn b/tags/archaea.mdwn
new file mode 100644
index 00000000..ed4ddb19
--- /dev/null
+++ b/tags/archaea.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged archaea"]]
+
+[[!inline pages="tagged(archaea)" actions="no" archive="yes"
+feedshow=10]]

Merge branch 'master' of ssh://charles-plessy-org.branchable.com
oburo
diff --git a/biblio/37307447.mdwn b/biblio/37307447.mdwn
new file mode 100644
index 00000000..4aa63d1a
--- /dev/null
+++ b/biblio/37307447.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Replitrons: A major group of eukaryotic transposons encoding HUH endonuclease."]]
+[[!tag repeat]]
+
+Craig RJ
+
+Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2301424120. doi:10.1073/pnas.2301424120
+
+Replitrons: A major group of eukaryotic transposons encoding HUH endonuclease.
+
+[[!pmid 37307447 desc="Copy-and-paste mechanism.  The transposon has short tandem repeats at its end, inserts a short copy of its downstream sequences in the new copy, and creates an even shorter duplication of its insertion site."]]

Misudo
diff --git a/biblio/37313762.mdwn b/biblio/37313762.mdwn
new file mode 100644
index 00000000..74c6c3a4
--- /dev/null
+++ b/biblio/37313762.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Evolutionary traces of miniaturization in a giant-Comparative anatomy of brain and brain nerves in Bathochordaeus stygius (Tunicata, Appendicularia)."]]
+[[!tag Oikopleura]]
+
+Zemann B, Le MV, Sherlock RE, Baum D, Katija K, Stach T.
+
+J Morphol. 2023 Jul;284(7):e21598. doi:10.1002/jmor.21598
+
+Evolutionary traces of miniaturization in a giant-Comparative anatomy of brain and brain nerves in _Bathochordaeus stygius_ (Tunicata, Appendicularia).
+
+[[!pmid 37313762 desc="102 cells in the brain, a number comparable to one in _O. doica_."]]
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 3791499b..404e868d 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -487,6 +487,8 @@ Anatomy
    are lost in appendicularians ([[Ferrández-Roldán and coll., 2021|biblio/34789899]]).
  - A 3D reconstitution of hatchlings and jufeniles was done by SEM tomography by
    [[Nishida and coll., 2021|biblio/33649401]].
+ - The brain of _B. stygius_ contains a number of cells comparable to the one of
+   _O. dioica_ ([[Zemann and coll., 2003|biblio/37313762]]).
 
 Physiology
 ----------

List culture paper of Paffenhöfer, 1973
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 3791499b..c0d7356e 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -725,6 +725,7 @@ Laboratory culture
 
 Culture protocols (incomplete list):
 
+ - [[G.-A. Paffenhöfer, 1973|biblio/10.1007_BF00391782]].
  - [[Fenaux and Gorsky (1985)|biblio/art0358]] published a method using a
    helicoidal paddle to stir the culture.
  - Rotating vessels: [[Sato and coll, 1999|biblio/10005415955]].

radio taiso
diff --git a/tags/synteny.mdwn b/tags/synteny.mdwn
index 12f886c3..f1b16968 100644
--- a/tags/synteny.mdwn
+++ b/tags/synteny.mdwn
@@ -55,7 +55,10 @@ chromosomes, but with randomized orders and orientations“ and call that
 phenomenon “mesosynteny”.
 
 Squid chromosomes still have synteny with scallop, but gene order is scrambled
-([[Albertin and coll., 2022|biblio/35508532]]).
+([[Albertin and coll., 2022|biblio/35508532]]).  Sets of syntenic orthologues
+present in separate chromosomes in some clades but irreversibly mixed in others
+allowed [[Schultz and coll. (2023)|biblio/37198475]] to predict that cnidarians
+branched at the base of the animal tree.
 
 At equal evolutionary distance, yeast microsynteny is lower than in animals,
 but higher than in plants ([[Li and coll., 2022|biblio/36334587]]).

Science is in the air...
diff --git a/biblio/26166067.mdwn b/biblio/26166067.mdwn
new file mode 100644
index 00000000..4fb52d73
--- /dev/null
+++ b/biblio/26166067.mdwn
@@ -0,0 +1,15 @@
+[[!meta title="Comparative genomics reveals conserved positioning of essential genomic clusters in highly rearranged Thermococcales chromosomes"]]
+[[!tag bacteria variants]]
+
+Matteo Cossu, Violette Da Cunha, Claire Toffano-Nioche, Patrick Forterre, Jacques Oberto
+
+Biochimie. 2015 Nov;118:313-21. doi:10.1016/j.biochi.2015.07.008
+
+Comparative genomics reveals conserved positioning of essential genomic clusters in highly rearranged Thermococcales chromosomes
+
+[[!pmid 26166067 desc="“The comparative genomics analysis presented here
+confirms the initial observation that Thermococcales chromosomes are highly
+rearranged. In these genomes, DNA sequence scrambling has reached such a high
+level that commonly observed prokaryotic chromosomal landmarks such as oriC and
+terC are no longer readily identifiable by measuring DNA composition
+biases.”"]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index fea90940..c86aa2db 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -67,7 +67,9 @@ The state of the art in bacterial genomics in 2000 was reviewed by
 
 A mixture of X-shaped dot-plot pattern and scrambling was reported by
 [[Zivanovic and coll. (2002)|biblio/11972326]] in a study of _Pyrococcus_
-genomes.
+genomes.  Scrambling in _Thermococcales_ appears to be fast enough that
+the usual compositional biases on both sides of the origin of replication
+do not have time to establish ([[Cossu and coll., 2015|biblio/26166067]]).
 
 ### Mechanism
 

Et ensuite, des râmen
diff --git a/biblio/11972326.mdwn b/biblio/11972326.mdwn
new file mode 100644
index 00000000..6793a9bf
--- /dev/null
+++ b/biblio/11972326.mdwn
@@ -0,0 +1,20 @@
+[[!meta title="Pyrococcus genome comparison evidences chromosome shuffling-driven evolution."]]
+[[!tag archaea variants]]
+
+Zivanovic Y, Lopez P, Philippe H, Forterre P.
+
+Nucleic Acids Res. 2002 May 1;30(9):1902-10. doi:10.1093/nar/30.9.1902
+
+Pyrococcus genome comparison evidences chromosome shuffling-driven evolution. 
+
+[[!pmid 11972326 desc="“the extent of rearrangement that occurred after the
+three species diverged is much lower between _P. abyssi_ and _P. horikoshii_
+than between these two species and _P. furiosus_.”  “[In _P. furiosus_, the
+distribution of IS elements] is clearly non-random, since 16 of them are
+located near shuffled segments boundaries, five are in completely scrambled
+regions and only two are found within an undisturbed segment.”  “the AT3 skew
+was less upset than the word skew (here GGTT), which was itself, as expected,
+less perturbed than the gene orientation skew”.  Coding genes were weakly
+colinear with the replication direction in _P. abissi_ and P. horikoshii_,
+especially for highly expressed genes, but this correlation was less evident or
+absent for _P. furiosus_."]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 4cd0a25e..fea90940 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -65,6 +65,10 @@ this phenomenon was confirmed by [[D'Iorio and Dewar, 2022|biblio/36261227]].
 The state of the art in bacterial genomics in 2000 was reviewed by
 [[Hughes|biblio/11380986]].
 
+A mixture of X-shaped dot-plot pattern and scrambling was reported by
+[[Zivanovic and coll. (2002)|biblio/11972326]] in a study of _Pyrococcus_
+genomes.
+
 ### Mechanism
 
 _Staggered breaks_ ([[Ranz and coll., 2007|bilbio/17550304]]) caused

Dans le train.
diff --git a/biblio/11380986.mdwn b/biblio/11380986.mdwn
new file mode 100644
index 00000000..50d61b35
--- /dev/null
+++ b/biblio/11380986.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Evaluating genome dynamics: the constraints on rearrangements within bacterial genomes."]]
+[[!tag bacteria variants review]]
+
+Hughes D.
+
+Genome Biol. 2000;1(6):REVIEWS0006. doi: 10.1186/gb-2000-1-6-reviews0006.
+
+Evaluating genome dynamics: the constraints on rearrangements within bacterial genomes.
+
+[[!pmid 11380986 desc="Review reporting that “almost all rearrangements conserve the axis of chromosome replication”."]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 9826c13b..4cd0a25e 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -62,6 +62,8 @@ in dot-plots by [[Eisen and coll (2000)|biblio/11178265]] and [[Tillier and
 Collins (2000)|biblio/11017076]].  Both reports suggest that the patterns are
 generated by inversions centered on origins and termini.  The prevalence of
 this phenomenon was confirmed by [[D'Iorio and Dewar, 2022|biblio/36261227]].
+The state of the art in bacterial genomics in 2000 was reviewed by
+[[Hughes|biblio/11380986]].
 
 ### Mechanism
 

Assis dans un parc
diff --git a/biblio/11017076.mdwn b/biblio/11017076.mdwn
new file mode 100644
index 00000000..a0bc955f
--- /dev/null
+++ b/biblio/11017076.mdwn
@@ -0,0 +1,15 @@
+[[!meta title="Genome rearrangement by replication-directed translocation."]]
+[[!tag bacteria variants]]
+
+Tillier ER, Collins RA
+
+Nat Genet. 2000 Oct;26(2):195-7. doi:10.1038/79918
+
+Genome rearrangement by replication-directed translocation.
+
+[[!pmid 11017076 desc="X-shaped patterns in whole-genome comparisons dot plots.
+“Single-gene inversions occurred at 12 of 44 boundaries [of repositioned blocks
+of genes] identified in _Chlamydia_, compared with only 13 local inversions
+adjacent to any of the 643 genes not at a boundary”  Proposes that
+origin-centered inversions caused by replication forks can generate the
+patterns."]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 285f087a..9826c13b 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -58,10 +58,10 @@ inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
 human/mouse comparisons, median length 814. 
 
 In bacteria, rearrangements have been reported to generate a X-shaped pattern
-in dot-plots by [[Eisen and coll (2000)|biblio/11178265]].  The authors suggest
-that the patterns are generated by inversions centered on origins and termini.
-The prevalence of this phenomenon was confirmed by [[D'Iorio and Dewar,
-2022|biblio/36261227]].
+in dot-plots by [[Eisen and coll (2000)|biblio/11178265]] and [[Tillier and
+Collins (2000)|biblio/11017076]].  Both reports suggest that the patterns are
+generated by inversions centered on origins and termini.  The prevalence of
+this phenomenon was confirmed by [[D'Iorio and Dewar, 2022|biblio/36261227]].
 
 ### Mechanism
 

Dans le train
diff --git a/biblio/36261227.mdwn b/biblio/36261227.mdwn
new file mode 100644
index 00000000..65f63bcb
--- /dev/null
+++ b/biblio/36261227.mdwn
@@ -0,0 +1,18 @@
+[[!meta title="Replication-associated inversions are the dominant form of bacterial chromosome structural variation."]]
+[[!tag bacteria variants]]
+
+D'Iorio M, Dewar K.
+
+Life Sci Alliance. 2022 Oct 19;6(1):e202201434. doi:10.26508/lsa.202201434
+
+Replication-associated inversions are the dominant form of bacterial chromosome structural variation.
+
+[[!pmid 36261227 desc="“We assessed a total of 313 species that were
+represented by 10 or more complete genome sequences”  “A genoform in this work
+refers to a group of collinear chromosomal sequences within a species with at
+least 80% sequence similarity and without an inversion spanning more than 50
+Kb.”  “In Gammaproteobacteria, the species Haemophilus influenzae, Haemophilus
+parainfluenzae, and Mannheimia haemolytica all contained inversions that were
+predominantly located away from the replication origin. We also observed
+species that were consistently offset from symmetry in one direction such as in
+B. pertussis”"]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 015b7c21..285f087a 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -60,6 +60,8 @@ human/mouse comparisons, median length 814.
 In bacteria, rearrangements have been reported to generate a X-shaped pattern
 in dot-plots by [[Eisen and coll (2000)|biblio/11178265]].  The authors suggest
 that the patterns are generated by inversions centered on origins and termini.
+The prevalence of this phenomenon was confirmed by [[D'Iorio and Dewar,
+2022|biblio/36261227]].
 
 ### Mechanism
 

Encore plus de café
diff --git a/biblio/11178265.mdwn b/biblio/11178265.mdwn
index 17aec71b..848e9444 100644
--- a/biblio/11178265.mdwn
+++ b/biblio/11178265.mdwn
@@ -7,4 +7,4 @@ Genome Biol. 2000;1(6):RESEARCH0011. doi:10.1186/gb-2000-1-6-research0011
 
 Evidence for symmetric chromosomal inversions around the replication origin in bacteria.
 
-[[!pmid 11178265 desc="X-shaped pattern observed in line plots of pairwise comparisons between E. coli and V. cholerae, or Streptococcus or Mycobacterium species."]]
+[[!pmid 11178265 desc="X-shaped pattern observed in line plots of pairwise comparisons between E. coli and V. cholerae, or Streptococcus or Mycobacterium species.  The patterns might have been generated by inversions centered on origins and termini of replication."]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index a89e2583..015b7c21 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -58,7 +58,8 @@ inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
 human/mouse comparisons, median length 814. 
 
 In bacteria, rearrangements have been reported to generate a X-shaped pattern
-in dot-plots by [[Eisen and coll (2000)|biblio/11178265]].
+in dot-plots by [[Eisen and coll (2000)|biblio/11178265]].  The authors suggest
+that the patterns are generated by inversions centered on origins and termini.
 
 ### Mechanism
 

Café
diff --git a/biblio/11178265.mdwn b/biblio/11178265.mdwn
new file mode 100644
index 00000000..17aec71b
--- /dev/null
+++ b/biblio/11178265.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Evidence for symmetric chromosomal inversions around the replication origin in bacteria."]]
+[[!tag variants bacteria]]
+
+Eisen JA, Heidelberg JF, White O, Salzberg SL.
+
+Genome Biol. 2000;1(6):RESEARCH0011. doi:10.1186/gb-2000-1-6-research0011
+
+Evidence for symmetric chromosomal inversions around the replication origin in bacteria.
+
+[[!pmid 11178265 desc="X-shaped pattern observed in line plots of pairwise comparisons between E. coli and V. cholerae, or Streptococcus or Mycobacterium species."]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 222faccd..a89e2583 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -57,6 +57,9 @@ inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
 [[Kent and coll., 2003|biblio/14500911]] reported 2 inversions per Mbp in
 human/mouse comparisons, median length 814. 
 
+In bacteria, rearrangements have been reported to generate a X-shaped pattern
+in dot-plots by [[Eisen and coll (2000)|biblio/11178265]].
+
 ### Mechanism
 
 _Staggered breaks_ ([[Ranz and coll., 2007|bilbio/17550304]]) caused

Café
diff --git a/biblio/37137302.mdwn b/biblio/37137302.mdwn
new file mode 100644
index 00000000..5b0ccce0
--- /dev/null
+++ b/biblio/37137302.mdwn
@@ -0,0 +1,29 @@
+[[!meta title="Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells"]]
+[[!tag yeast synthetic]]
+
+Gvozdenov Z, Barcutean Z, Struhl K
+
+Mol Cell. 2023 Apr 24:S1097-2765(23)00254-X. 10.1016/j.molcel.2023.04.010
+
+Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells
+
+[[!pmid 37137302 desc="Circular chromosome of 27 kbp comprising 18 kbp of random
+sequences.  “the major class of nucleosomes average 147 bp (range 143–180),
+i.e., the expected size”  “nucleosome occupancy on [the artificial chromosome]
+is 1.42-fold higher than on yeast genomic DNA”  “when compared with yeast
+genomic chromatin, [artificial] chro- matin is characterized by fewer
+[nucleotide-depleted regions], fewer highly positioned nucleosomes, and little
+nucleosome phasing, although nucleosome positioning is nonrandom.”
+“Transcription from all regions of random-sequence DNA leads to similar RNA
+levels that are roughly comparable to that of the majority of mRNA expressed
+from yeast genomic DNA”  “transcription from randomsequence DNA is more
+variable than from genomic DNA”  “decay rates of RNAs expressed from
+randomsequence DNA are 3-fold faster”  “RNAs expressed from [the artificial
+chrmosome] are polyadenylated throughout the entire region of random-sequence
+DNA”  “the choice of poly(A) sites by the cleavage/polyadenylation machinery is
+roughly similar for RNAs expressed from random-sequence and genomic DNAs.” “The
+50 isoform pattern from random-sequence DNA resembles that observed from yeast
+genomic regions not corresponding to 50 UTRs”  “The level of newly synthesized
+RNA from random-sequence DNA is in excellent accord with calculated estimates
+that only 10%–20% of elongating RNA Pol II molecules generate mRNAs, such the
+remaining 80%–90% represent transcriptional noise”"]]
diff --git a/tags/synthetic.mdwn b/tags/synthetic.mdwn
index 120fc94d..07a6d002 100644
--- a/tags/synthetic.mdwn
+++ b/tags/synthetic.mdwn
@@ -12,8 +12,12 @@ Example of neochromosome synthesis in yeast: [[Postma and coll., 2021|biblio/334
 
 Artificial genome scrambling in yeast 2.0 with loxPsym sites: [[Brooks and coll, 2022|biblio/35239377]].
 
+Artificial 18 kbp random chromosome in yeast: [[Gvozdenov, Barcutean and Struhl, 2023|biblio/37137302]].
+
 ## Pathway
 
-Increasing the copy number of the endogenous aldehyde dehydrogenase Hfd1 allowed [[Hu, Yu and Ye, 2022|biblio/35880393]] to obtain the best yield of conversion of retinol to retinoic acid..
+Increasing the copy number of the endogenous aldehyde dehydrogenase Hfd1
+allowed [[Hu, Yu and Ye, 2022|biblio/35880393]] to obtain the best yield of
+conversion of retinol to retinoic acid.
 
 [[!inline pages="tagged(synthethic)" limit=0]]

Dans l'avion
diff --git a/biblio/36161960.mdwn b/biblio/36161960.mdwn
new file mode 100644
index 00000000..38c577af
--- /dev/null
+++ b/biblio/36161960.mdwn
@@ -0,0 +1,33 @@
+[[!meta title="Evolution of the ancestral mammalian karyotype and syntenic regions."]]
+[[!tag synteny]]
+
+Damas J, Corbo M, Kim J, Turner-Maier J, Farré M, Larkin DM, Ryder OA, Steiner C, Houck ML, Hall S, Shiue L, Thomas S, Swale T, Daly M, Korlach J, Uliano-Silva M, Mazzoni CJ, Birren BW, Genereux DP, Johnson J, Lindblad-Toh K, Karlsson EK, Nweeia MT, Johnson RN; Zoonomia Consortium; Lewin HA.
+
+Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2209139119. doi: 10.1073/pnas.2209139119
+
+Evolution of the ancestral mammalian karyotype and syntenic regions.
+
+[[!pmid 36161960 desc="Analyses the number of breakpoints between nodes of the
+phylogenetic tree, thanks to ancestral genome reconstruction.  “2 extant
+mammals (Dataset S1), representing all 19 eutherian, 3 marsupial, and the
+monotreme orders, were used to reconstruct ancestral karyotypes at 16 nodes of
+the mammalian phylogeny”  “We used DESCHRAMBLER to generate reconstructed
+ancestral chromosome fragments (RACFs) for each of 16 mammalian ancestors at
+300-kbp syntenic fragment (SF) resolution”  “The reconstructed mammalian
+ancestor karyotype has 19 autosomes plus X, except for the cattle genome-based
+reconstruction, which has two fewer chromosomes and the lowest total
+reconstruction length”  “The differences between the mammalian and therian (n =
+17 + X) ancestors’ chromosomes resulted from 96 chromosomal rearrangements over
+18 My.”  “The eutherian ancestor (n = 19 + X) is the most recent common
+ancestor of the three lineages represented by the reference genomes. Its
+karyotype evolved from that of the therian ancestor as a result of 124
+chromosomal rearrangements over 53 My.”  “We identified 323, 262, and 257 EBRs
+that occurred along the lineage from the mammalian ancestor to the human,
+sloth, and cattle genomes, respectively”  “Inversions were most frequent,
+accounting for 76 to 85% of all rearrangements identified for each lineage”
+“The highest number of interchromosomal rearrangements (i.e., fissions and
+fusions) was observed on the branch from the therian to the eutherian ancestor
+(n = 30)”  “We observed that 9 of 14 small MAMs have 1:1 orthology to chicken
+chromosomes (GGA) and reconstructed chromosomes of the avian and amniote
+ancestors”  “EBRs were found to have a significantly higher density of
+repeats”"]]
diff --git a/tags/synteny.mdwn b/tags/synteny.mdwn
index d9fd0d0f..12f886c3 100644
--- a/tags/synteny.mdwn
+++ b/tags/synteny.mdwn
@@ -62,7 +62,9 @@ but higher than in plants ([[Li and coll., 2022|biblio/36334587]]).
 
 ### Ancestral karyotpyes
 
- - The ancestral mammalian genome has 30 chromosomes ([[Zhou and coll., 2021|biblio/33408411]]).
+ - The ancestral mammalian genome has 30 chromosomes ([[Zhou and coll.,
+   2021|biblio/33408411]]), or 19 + X ([[Damas and coll.,
+   2022|biblio/36161960]]).
 
  - The ancestral chordate has 17 chromosomes according to amphioxus assemblies
   ([[Putnam and coll, 2008|biblio/18563158]], [[Simakov and coll., 2020|biblio/32313176]]).

Café
diff --git a/biblio/37198475.mdwn b/biblio/37198475.mdwn
new file mode 100644
index 00000000..9be501f8
--- /dev/null
+++ b/biblio/37198475.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Ancient gene linkages support ctenophores as sister to other animals."]]
+[[!tag synteny]]
+
+Schultz DT, Haddock SHD, Bredeson JV, Green RE, Simakov O, Rokhsar DS. 
+
+Nature. 2023 May 17. doi:10.1038/s41586-023-05936-6
+
+Ancient gene linkages support ctenophores as sister to other animals.
+
+[[!pmid 37198475 desc="Gene linkages found in all metazoans and single-celled outgroups are irreversibly merged (fusion followed by mixing) in Porifera, Cnidaria and Bilateria, supporting the idea that Ctenophora are sister to them."]]

CAGE
diff --git a/biblio/10.1101_2023.04.27.538568.mdwn b/biblio/10.1101_2023.04.27.538568.mdwn
new file mode 100644
index 00000000..07acf6f9
--- /dev/null
+++ b/biblio/10.1101_2023.04.27.538568.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Abundant capped RNAs are derived from mRNA cleavage at 3’UTR G-Quadruplexes"]]
+[[!tag CAGE 3′_UTR]]
+
+Nejc Haberman, Holly Digby, Rupert Faraway, Rebecca Cheung, Andrew M Jobbins, Callum Parr, Kayoko Yasuzawa, Takeya Kasukawa, Chi Wai Yip, Masaki Kato, Hazuki Takahashi, Piero Carninci, Santiago Vernia, Jernej Ule, Christopher R Sibley, Aida Martinez-Sanchez, Boris Lenhard
+
+bioRxiv 2023.04.27.538568; doi:10.1101/2023.04.27.538568
+
+Abundant capped RNAs are derived from mRNA cleavage at 3’UTR G-Quadruplexes
+
+[[!doi 10.1101/2023.04.27.538568  desc="3′ peaks colocalising with a G-rich motif are now explained by AGO2-mediated cleavage of G-quadruplex regions and recapping.  The 3′ fragments and the mRNA did not localise equally in the cell when imaged by in-situ hybridisation."]]

po
diff --git "a/Debian/debi\303\242neries/markdown2.en.po" "b/Debian/debi\303\242neries/markdown2.en.po"
index 9773d7d7..da40e345 100644
--- "a/Debian/debi\303\242neries/markdown2.en.po"
+++ "b/Debian/debi\303\242neries/markdown2.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2023-05-11 00:02+0000\n"
-"PO-Revision-Date: 2023-05-11 08:58+0900\n"
+"PO-Revision-Date: 2023-05-11 09:03+0900\n"
 "Last-Translator: \n"
 "Language-Team: \n"
 "Language: en\n"
@@ -32,8 +32,7 @@ msgid "[[!tag Debian]]\n"
 msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
-#, fuzzy, no-wrap
-#| msgid "[[!meta title=\"Plussez pour patcher Firefox pour afficher du Markdown ?\"]]\n"
+#, no-wrap
 msgid "[[!meta title=\"Plussez pour patcher Firefox pour afficher du Markdown.\"]]\n"
 msgstr "[[!meta title=\"Upvote to patch Firefox to render Markdown\"]]\n"
 

updated PO files
diff --git "a/Debian/debi\303\242neries/markdown2.en.po" "b/Debian/debi\303\242neries/markdown2.en.po"
index b85db619..9773d7d7 100644
--- "a/Debian/debi\303\242neries/markdown2.en.po"
+++ "b/Debian/debi\303\242neries/markdown2.en.po"
@@ -6,7 +6,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2023-05-10 23:51+0000\n"
+"POT-Creation-Date: 2023-05-11 00:02+0000\n"
 "PO-Revision-Date: 2023-05-11 08:58+0900\n"
 "Last-Translator: \n"
 "Language-Team: \n"
@@ -32,24 +32,27 @@ msgid "[[!tag Debian]]\n"
 msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
-#, no-wrap
-msgid "[[!meta title=\"Plussez pour patcher Firefox pour afficher du Markdown ?\"]]\n"
+#, fuzzy, no-wrap
+#| msgid "[[!meta title=\"Plussez pour patcher Firefox pour afficher du Markdown ?\"]]\n"
+msgid "[[!meta title=\"Plussez pour patcher Firefox pour afficher du Markdown.\"]]\n"
 msgstr "[[!meta title=\"Upvote to patch Firefox to render Markdown\"]]\n"
 
 #. type: Plain text
 msgid ""
-"J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont le type média est "
-"_text/markdown_, il le propose au téléchargement, alors que les autres navigateurs l'affichent "
-"comme un fichier texte."
+"J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont "
+"le type média est _text/markdown_, il le propose au téléchargement, alors "
+"que les autres navigateurs l'affichent comme un fichier texte."
 msgstr ""
-"I [[previously wrote|markdown]] that when Firefox receives a file whose media type is _text/"
-"markdown_, it prompts the user to download it, whereas other browsers display rendered results."
+"I [[previously wrote|markdown]] that when Firefox receives a file whose "
+"media type is _text/markdown_, it prompts the user to download it, whereas "
+"other browsers display rendered results."
 
 #. type: Plain text
 msgid ""
-"Il est maintenant possible de plusser sur [connect.mozilla.org](https://connect.mozilla.org/t5/"
-"ideas/display-markdown-files/idi-p/6000)  pour demander que Firefox formatte le _markdown_ par "
-"défaut."
+"Il est maintenant possible de plusser sur [connect.mozilla.org](https://"
+"connect.mozilla.org/t5/ideas/display-markdown-files/idi-p/6000)  pour "
+"demander que Firefox formatte le _markdown_ par défaut."
 msgstr ""
-"Now it is possible to upvote a proposal on [connect.mozilla.org](https://connect.mozilla.org/"
-"t5/ideas/display-markdown-files/idi-p/6000) asking that Firefox renders Markdown by default."
+"Now it is possible to upvote a proposal on [connect.mozilla.org](https://"
+"connect.mozilla.org/t5/ideas/display-markdown-files/idi-p/6000) asking that "
+"Firefox renders Markdown by default."

Correction du titre.
diff --git "a/Debian/debi\303\242neries/markdown2.mdwn" "b/Debian/debi\303\242neries/markdown2.mdwn"
index 4191be52..cc9d9ceb 100644
--- "a/Debian/debi\303\242neries/markdown2.mdwn"
+++ "b/Debian/debi\303\242neries/markdown2.mdwn"
@@ -2,7 +2,7 @@
 [[!meta updated="Thu, 11 May 2023 08:43:33 +0900"]]
 [[!tag Debian]]
 
-[[!meta title="Plussez pour patcher Firefox pour afficher du Markdown ?"]]
+[[!meta title="Plussez pour patcher Firefox pour afficher du Markdown."]]
 
 J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont le
 type média est _text/markdown_, il le propose au téléchargement, alors que les

upvote !
diff --git "a/Debian/debi\303\242neries/markdown2.en.po" "b/Debian/debi\303\242neries/markdown2.en.po"
index ad915ae4..b85db619 100644
--- "a/Debian/debi\303\242neries/markdown2.en.po"
+++ "b/Debian/debi\303\242neries/markdown2.en.po"
@@ -3,51 +3,53 @@
 # This file is distributed under the same license as the PACKAGE package.
 # FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
 #
-#, fuzzy
 msgid ""
 msgstr ""
-"Project-Id-Version: PACKAGE VERSION\n"
+"Project-Id-Version: \n"
 "POT-Creation-Date: 2023-05-10 23:51+0000\n"
-"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
-"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
-"Language-Team: LANGUAGE <LL@li.org>\n"
-"Language: \n"
+"PO-Revision-Date: 2023-05-11 08:58+0900\n"
+"Last-Translator: \n"
+"Language-Team: \n"
+"Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
+"X-Generator: Poedit 3.2.2\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta date=\"Thu, 11 May 2023 08:43:33 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta date=\"Thu, 11 May 2023 08:43:33 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta updated=\"Thu, 11 May 2023 08:43:33 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta updated=\"Thu, 11 May 2023 08:43:33 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!tag Debian]]\n"
-msgstr ""
+msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
 #, no-wrap
-msgid ""
-"[[!meta title=\"Plussez pour patcher Firefox pour afficher du "
-"Markdown ?\"]]\n"
-msgstr ""
+msgid "[[!meta title=\"Plussez pour patcher Firefox pour afficher du Markdown ?\"]]\n"
+msgstr "[[!meta title=\"Upvote to patch Firefox to render Markdown\"]]\n"
 
 #. type: Plain text
 msgid ""
-"J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont "
-"le type média est _text/markdown_, il le propose au téléchargement, alors "
-"que les autres navigateurs l'affichent comme un fichier texte."
+"J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont le type média est "
+"_text/markdown_, il le propose au téléchargement, alors que les autres navigateurs l'affichent "
+"comme un fichier texte."
 msgstr ""
+"I [[previously wrote|markdown]] that when Firefox receives a file whose media type is _text/"
+"markdown_, it prompts the user to download it, whereas other browsers display rendered results."
 
 #. type: Plain text
 msgid ""
-"Il est maintenant possible de plusser sur "
-"[connect.mozilla.org](https://connect.mozilla.org/t5/ideas/display-markdown-files/idi-p/6000)  "
-"pour demander que Firefox formatte le _markdown_ par défaut."
+"Il est maintenant possible de plusser sur [connect.mozilla.org](https://connect.mozilla.org/t5/"
+"ideas/display-markdown-files/idi-p/6000)  pour demander que Firefox formatte le _markdown_ par "
+"défaut."
 msgstr ""
+"Now it is possible to upvote a proposal on [connect.mozilla.org](https://connect.mozilla.org/"
+"t5/ideas/display-markdown-files/idi-p/6000) asking that Firefox renders Markdown by default."

updated PO files
diff --git "a/Debian/debi\303\242neries/markdown2.en.po" "b/Debian/debi\303\242neries/markdown2.en.po"
new file mode 100644
index 00000000..ad915ae4
--- /dev/null
+++ "b/Debian/debi\303\242neries/markdown2.en.po"
@@ -0,0 +1,53 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2023-05-10 23:51+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Thu, 11 May 2023 08:43:33 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Thu, 11 May 2023 08:43:33 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"[[!meta title=\"Plussez pour patcher Firefox pour afficher du "
+"Markdown ?\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont "
+"le type média est _text/markdown_, il le propose au téléchargement, alors "
+"que les autres navigateurs l'affichent comme un fichier texte."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Il est maintenant possible de plusser sur "
+"[connect.mozilla.org](https://connect.mozilla.org/t5/ideas/display-markdown-files/idi-p/6000)  "
+"pour demander que Firefox formatte le _markdown_ par défaut."
+msgstr ""
diff --git a/Joules.en.po b/Joules.en.po
index e0ffd0e2..51cf028b 100644
--- a/Joules.en.po
+++ b/Joules.en.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2023-05-09 13:37+0000\n"
+"POT-Creation-Date: 2023-05-10 23:51+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -43,6 +43,39 @@ msgid ""
 "Joules) sur Framapiaf."
 msgstr ""
 
+#. type: Title ##
+#, no-wrap
+msgid "En bref, par an:"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Travail: 100 ~ 350 GJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Avion: 20 ~ 40 GJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Voiture: 10 ~ 15 GJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Électricité: 5 GJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Nourriture (métabolisme): 3 GJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Gaz: 2 GJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Télécoms: 1 ~ 5 GJ (arrondis à la louche)"
+msgstr ""
+
 #. type: Title ##
 #, no-wrap
 msgid "Les transports"
@@ -62,7 +95,10 @@ msgid ""
 "Je roule en **voiture** 30 kilomètres par jour pour aller travailler, "
 "environ 20 jours par mois.  Avec une voiture hybride parcourant 25 "
 "kilomètres par litre, ça fait 24 litres par mois, donc environ 800 MJ par "
-"mois."
+"mois ou environ 10 GJ par an.  J'achète environ 50 litres d'essence par mois "
+"et hormis mes trajets aller travailler, nous sommes souvents trois dans la "
+"voiture.  Donc aller au travail représente les trois quarts de ce que je "
+"dépense en essence à titre individuel."
 msgstr ""
 
 #. type: Bullet: ' - '
@@ -89,7 +125,8 @@ msgid ""
 "litre (43 MJ/kg pour densité de 0.8).  La différence avec la voiture, c'est "
 "qu'on fait beaucoup plus de kilomètres en avion.  Je fais environ un voyage "
 "intercontinental (20 000 km) et quatre voyages domestiques entre Okinawa et "
-"Tôkyô (4 × 2000 km).  Donc environ 840 litres par an, donc 2 400 MJ."
+"Tôkyô (4 × 3000 km).  Donc environ 600 et 1200 litres par an, donc entre 20 "
+"et 40 GJ par an."
 msgstr ""
 
 #. type: Bullet: ' - '
@@ -195,9 +232,9 @@ msgid ""
 "serait plus que la moyenne au Japon ou en Amérique, on voit que la "
 "consommation résidentielle reste négligeable dans l'équation.  Entre 1000 et "
 "2000 personnes travaillent sur le campus.  On peut donc dire que chacun "
-"d'entre nous consomme de l'ordre de 360 GJ / an en moyenne, et que c'est "
-"probablement un peu surestimé.  En comparaison, je consomme 800 MJ * 12 ~= "
-"10 GJ d'énergie en carburant pour aller travailler."
+"d'entre nous consomme de l'ordre de 100 à 360 GJ / an en moyenne, et que "
+"c'est probablement un peu surestimé.  En comparaison, je consomme 800 MJ * "
+"12 ~= 10 GJ d'énergie en carburant pour aller travailler."
 msgstr ""
 
 #. type: Title ##
@@ -229,3 +266,35 @@ msgid ""
 "Le coût énergétique de la synthèse et manufacture du plastique est estimé à "
 "18 MJ / kg de plastique."
 msgstr ""
+
+#. type: Plain text
+msgid "le vin"
+msgstr ""
+
+#. type: Plain text
+msgid "Aller au travail en vélo"
+msgstr ""
+
+#. type: Plain text
+msgid "une vie plus bourgeoise ? Une vie plus maigre ?"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Consommation annuelle de pétrole (4.2 10e20 J)/ Énergie envoyée par le "
+"soleil: 6,2 × 1020 J \tl'énergie totale du Soleil qui atteint la Terre en "
+"une heure. (https://fr.wikipedia.org/wiki/Ordres_de_grandeur_d"
+"%27%C3%A9nergie)"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"L'énergie cinétique d'un 1kg de matière à la vitesse de libération (7.9 km/"
+"s)  est d'un peu plus de 30 MJ.  Il faut brûler un litre d'essence pour "
+"dégager cette énergie.  Malheureusement, comme on n'a pas encore inventé "
+"mieux, il faut aussi transporter l'essence et le moteur, ce qui alourdit "
+"considérablement l'ensemble, au point de doubler le poids et donc l'énergie "
+"demandée, et donc le poids, et donc l'énergie demandée, etc.  Note: la "
+"différence d'énergie potentielle dans le champs de gravité terrestre est "
+"largement inférieure à la quantité d'énergie cinétique nécessaire."
+msgstr ""

Plussons!
diff --git "a/Debian/debi\303\242neries/markdown2.mdwn" "b/Debian/debi\303\242neries/markdown2.mdwn"
new file mode 100644
index 00000000..4191be52
--- /dev/null
+++ "b/Debian/debi\303\242neries/markdown2.mdwn"
@@ -0,0 +1,13 @@
+[[!meta date="Thu, 11 May 2023 08:43:33 +0900"]]
+[[!meta updated="Thu, 11 May 2023 08:43:33 +0900"]]
+[[!tag Debian]]
+
+[[!meta title="Plussez pour patcher Firefox pour afficher du Markdown ?"]]
+
+J'ai [[écrit auparavant|markdown]] que quand Firefox reçoit un fichier dont le
+type média est _text/markdown_, il le propose au téléchargement, alors que les
+autres navigateurs l'affichent comme un fichier texte.
+
+Il est maintenant possible de plusser sur
+[connect.mozilla.org](https://connect.mozilla.org/t5/ideas/display-markdown-files/idi-p/6000)
+pour demander que Firefox formatte le _markdown_ par défaut.

En bref par an.
diff --git a/Joules.md b/Joules.md
index 9b55565f..4ff4901a 100644
--- a/Joules.md
+++ b/Joules.md
@@ -13,6 +13,16 @@ l'unité du Système International, pour mieux comparer.  Je vais poster chacun
 de mes calculs avec le tag [#Joules](https://framapiaf.org/tags/Joules) sur
 Framapiaf.
 
+## En bref, par an:
+
+ - Travail: 100 ~ 350 GJ.
+ - Avion: 20 ~ 40 GJ.
+ - Voiture: 10 ~ 15 GJ.
+ - Électricité: 5 GJ.
+ - Nourriture (métabolisme): 3 GJ.
+ - Gaz: 2 GJ.
+ - Télécoms: 1 ~ 5 GJ (arrondis à la louche)
+
 ## Les transports
 
  - « _L'énergie contenue dans l'essence est d'environ 33 600 000 joules par
@@ -22,7 +32,11 @@ Framapiaf.
 
  - Je roule en **voiture** 30 kilomètres par jour pour aller travailler,
    environ 20 jours par mois.  Avec une voiture hybride parcourant 25
-   kilomètres par litre, ça fait 24 litres par mois, donc environ 800 MJ par mois.
+   kilomètres par litre, ça fait 24 litres par mois, donc environ 800 MJ par
+   mois ou environ 10 GJ par an.  J'achète environ 50 litres d'essence
+   par mois et hormis mes trajets aller travailler, nous sommes souvents
+   trois dans la voiture.  Donc aller au travail représente les trois quarts
+   de ce que je dépense en essence à titre individuel.
 
  - Pour parcourir un kilomètre en **train** je dois dépenser entre 10 et 20 yens.
    Çela me côute environ autant en essence quand je pends la voiture, mais
@@ -40,7 +54,8 @@ Framapiaf.
    litre (43 MJ/kg pour densité de 0.8).  La différence avec la voiture, c'est
    qu'on fait beaucoup plus de kilomètres en avion.  Je fais environ un voyage
    intercontinental (20 000 km) et quatre voyages domestiques entre Okinawa et
-   Tôkyô (4 × 2000 km).  Donc environ 840 litres par an, donc 2 400 MJ.
+   Tôkyô (4 × 3000 km).  Donc environ 600 et 1200 litres par an, donc entre 20
+   et 40 GJ par an.
 
  - Article intéressant comparant la dépense énergétique de chaque moyen de
    transport.  La dépense du corps humain est estmiée via la consommation
@@ -112,7 +127,7 @@ Framapiaf.
    plus que la moyenne au Japon ou en Amérique, on voit que la consommation
    résidentielle reste négligeable dans l'équation.  Entre 1000 et 2000 personnes
    travaillent sur le campus.  On peut donc dire que chacun d'entre nous consomme
-   de l'ordre de 360 GJ / an en moyenne, et que c'est probablement un peu
+   de l'ordre de 100 à 360 GJ / an en moyenne, et que c'est probablement un peu
    surestimé.  En comparaison, je consomme 800 MJ * 12 ~= 10 GJ d'énergie en
    carburant pour aller travailler.
 
@@ -129,3 +144,20 @@ estimé à 1/2 ou 1/3 du coût total. (à vérifier)
 
 Le coût énergétique de la synthèse et manufacture du plastique est
 estimé à 18 MJ / kg de plastique.
+
+le vin
+
+Aller au travail en vélo
+
+une vie plus bourgeoise ?  Une vie plus maigre ?
+
+Consommation annuelle de pétrole (4.2 10e20 J)/ Énergie envoyée par le soleil: 6,2 × 1020 J 	l'énergie totale du Soleil qui atteint la Terre en une heure. (https://fr.wikipedia.org/wiki/Ordres_de_grandeur_d%27%C3%A9nergie)
+
+L'énergie cinétique d'un 1kg de matière à la vitesse de libération (7.9 km/s)
+est d'un peu plus de 30 MJ.  Il faut brûler un litre d'essence pour dégager
+cette énergie.  Malheureusement, comme on n'a pas encore inventé mieux, il faut
+aussi transporter l'essence et le moteur, ce qui alourdit considérablement
+l'ensemble, au point de doubler le poids et donc l'énergie demandée, et donc le
+poids, et donc l'énergie demandée, etc.  Note: la différence d'énergie
+potentielle dans le champs de gravité terrestre est largement inférieure à la
+quantité d'énergie cinétique nécessaire.

updated PO files
diff --git a/Joules.en.po b/Joules.en.po
index 75b3cab6..e0ffd0e2 100644
--- a/Joules.en.po
+++ b/Joules.en.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2023-05-09 13:35+0000\n"
+"POT-Creation-Date: 2023-05-09 13:37+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -229,35 +229,3 @@ msgid ""
 "Le coût énergétique de la synthèse et manufacture du plastique est estimé à "
 "18 MJ / kg de plastique."
 msgstr ""
-
-#. type: Plain text
-msgid "le vin"
-msgstr ""
-
-#. type: Plain text
-msgid "Aller au travail en vélo"
-msgstr ""
-
-#. type: Plain text
-msgid "une vie plus bourgeoise ? Une vie plus maigre ?"
-msgstr ""
-
-#. type: Plain text
-msgid ""
-"Consommation annuelle de pétrole (4.2 10e20 J)/ Énergie envoyée par le "
-"soleil: 6,2 × 1020 J \tl'énergie totale du Soleil qui atteint la Terre en "
-"une heure. (https://fr.wikipedia.org/wiki/Ordres_de_grandeur_d"
-"%27%C3%A9nergie)"
-msgstr ""
-
-#. type: Plain text
-msgid ""
-"L'énergie cinétique d'un 1kg de matière à la vitesse de libération (7.9 km/"
-"s)  est d'un peu plus de 30 MJ.  Il faut brûler un litre d'essence pour "
-"dégager cette énergie.  Malheureusement, comme on n'a pas encore inventé "
-"mieux, il faut aussi transporter l'essence et le moteur, ce qui alourdit "
-"considérablement l'ensemble, au point de doubler le poids et donc l'énergie "
-"demandée, et donc le poids, et donc l'énergie demandée, etc.  Note: la "
-"différence d'énergie potentielle dans le champs de gravité terrestre est "
-"largement inférieure à la quantité d'énergie cinétique nécessaire."
-msgstr ""

On enlève le brouillon
diff --git a/Joules.md b/Joules.md
index 8194cf9e..9b55565f 100644
--- a/Joules.md
+++ b/Joules.md
@@ -129,20 +129,3 @@ estimé à 1/2 ou 1/3 du coût total. (à vérifier)
 
 Le coût énergétique de la synthèse et manufacture du plastique est
 estimé à 18 MJ / kg de plastique.
-
-le vin
-
-Aller au travail en vélo
-
-une vie plus bourgeoise ?  Une vie plus maigre ?
-
-Consommation annuelle de pétrole (4.2 10e20 J)/ Énergie envoyée par le soleil: 6,2 × 1020 J 	l'énergie totale du Soleil qui atteint la Terre en une heure. (https://fr.wikipedia.org/wiki/Ordres_de_grandeur_d%27%C3%A9nergie)
-
-L'énergie cinétique d'un 1kg de matière à la vitesse de libération (7.9 km/s)
-est d'un peu plus de 30 MJ.  Il faut brûler un litre d'essence pour dégager
-cette énergie.  Malheureusement, comme on n'a pas encore inventé mieux, il faut
-aussi transporter l'essence et le moteur, ce qui alourdit considérablement
-l'ensemble, au point de doubler le poids et donc l'énergie demandée, et donc le
-poids, et donc l'énergie demandée, etc.  Note: la différence d'énergie
-potentielle dans le champs de gravité terrestre est largement inférieure à la
-quantité d'énergie cinétique nécessaire.

updated PO files
diff --git a/Joules.en.po b/Joules.en.po
index 2183e164..75b3cab6 100644
--- a/Joules.en.po
+++ b/Joules.en.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2022-12-16 12:58+0000\n"
+"POT-Creation-Date: 2023-05-09 13:35+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -43,6 +43,11 @@ msgid ""
 "Joules) sur Framapiaf."
 msgstr ""
 
+#. type: Title ##
+#, no-wrap
+msgid "Les transports"
+msgstr ""
+
 #. type: Bullet: ' - '
 msgid ""
 "« _L'énergie contenue dans l'essence est d'environ 33 600 000 joules par "
@@ -54,42 +59,75 @@ msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Je roule 30 kilomètres par jour pour aller travailler, environ 20 jours par "
-"mois, avec une voiture parcourant 25 kilomètres par litre, ça fait 24 litres "
-"par mois, donc environ 800 MJ par mois."
+"Je roule en **voiture** 30 kilomètres par jour pour aller travailler, "
+"environ 20 jours par mois.  Avec une voiture hybride parcourant 25 "
+"kilomètres par litre, ça fait 24 litres par mois, donc environ 800 MJ par "
+"mois."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Je n'ai aucune idée précise de la quantité d'énergie que j'ingère chaque "
-"jour.  Mon pèse-personne, calibré pour une masse corporelle japonaise, me "
-"recommande de manger 1700 kilocalories par jour.  C'est gross-modo mon "
-"métabolisme de base d'après les chiffres résumés par l'ANSES dans on avis "
-"suivant la saisine 2012-SA-0103.  Dans ce même document, les besoins d'un "
-"homme de mon age « modérément actif » sont un peu au dessus de 2500 kcal par "
-"jour.  Une calorie vaut environ 4.2 Joules.  10 000 kJoules valent un peu "
-"moins de 2400 kcal.  En approximant ça à ma consommation quotidienne, j'en "
-"déduis que je consomme environ 300 MJ par mois.  Reste à savoir: l'énergie "
-"dépensée pour me fournir cette nourriture…"
+"Pour parcourir un kilomètre en **train** je dois dépenser entre 10 et 20 "
+"yens.  Çela me côute environ autant en essence quand je pends la voiture, "
+"mais c'est sans compter la rentabilisation de la voiture et les taxes sur la "
+"propriété et le contrôle technique (et pour d'autres, le prix du parking).  "
+"Mes trajets en voiture consomment (voir plus haut) 1/25 L par km, c'est à "
+"dire environ 825 kJ par km, ou 825 J / m.  C'est très proche des chiffres "
+"annoncés par Die Bahn pour les transports par train régionaux en Allemagne."
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "   https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/progress-in-climate-protection/energy-efficiency-increased/\n"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"On estime la consommation des **avions** modernes entre 2 et 4 litres de "
+"kérosène par 100 kilomètres et par passager.  Son pouvoir calorifique n'est "
+"pas très différent ce celui de l'essence de nos voitures, mettons 34 MJ par "
+"litre (43 MJ/kg pour densité de 0.8).  La différence avec la voiture, c'est "
+"qu'on fait beaucoup plus de kilomètres en avion.  Je fais environ un voyage "
+"intercontinental (20 000 km) et quatre voyages domestiques entre Okinawa et "
+"Tôkyô (4 × 2000 km).  Donc environ 840 litres par an, donc 2 400 MJ."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Article intéressant comparant la dépense énergétique de chaque moyen de "
+"transport.  La dépense du corps humain est estmiée via la consommation "
+"d'oxygène, bien étudiée.  Le rendement musculaire n'est pas très haut : les "
+"trois quarts de l'énergie dépensée se dissipent en chaleur, et le reste est "
+"disponible pour le mouvement."
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "   http://www.economiematin.fr/news-consommation-transports-industrie-services-energie\n"
+msgstr ""
+
+#. type: Title ##
+#, no-wrap
+msgid "Le logement"
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
 "Pour nous chauffer et nous rafraîchir, pour utiliser nos appareils électro-"
 "ménagers, et nos médias à écrans, nous consommons environ 300 kWh "
-"d'électricité par mois.  Mettons que ça fait 100 pour moi.  Une puissance de "
-"1 Watt, c'est 1 Joule par seconde.  Donc 100 kWh font 360 MJ.  Une grande "
-"partie de cette énergie provient de centrales à charbon et il faut de "
-"l'énergie pour l'extraire et le transporter.  Une partie de l'électricité "
-"produite se dissipe en chaleur dans le réseau électrique.  Ma consommation "
-"réelle est donc supérieure à ce que m'annonce le compteur."
+"d'**électricité** par mois.  Mettons que ça fait 100 pour moi.  Une "
+"puissance de 1 Watt, c'est 1 Joule par seconde.  Donc 100 kWh font 360 MJ.  "
+"Une grande partie de cette énergie provient de centrales à charbon et il "
+"faut de l'énergie pour l'extraire et le transporter.  Une partie de "
+"l'électricité produite se dissipe en chaleur dans le réseau électrique.  Ma "
+"consommation réelle est donc supérieure à ce que m'annonce le compteur."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
 "Pour cuisiner, chauffer notre eau de lavage, et sécher nos habits, nous "
-"utilisons environ 12 mètres cube de gaz par mois, donc 4 pour ma part.  Il "
-"existe différents types de gaz domestique, et une partie de l'énergie "
+"utilisons environ 12 mètres cube de **gaz** par mois, donc 4 pour ma part.  "
+"Il existe différents types de gaz domestique, et une partie de l'énergie "
 "dégagée par la combustion se perd par l'émission de vapeur d'eau.  Les "
 "chiffres donnés sur Wikipédia sont pour une température ambiante de zéro "
 "degrés, ce qui n'est pas le cas dans notre appartement.  Néanmoins, une "
@@ -101,19 +139,8 @@ msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"On estime la consommation des avions modernes entre 2 et 4 litres de "
-"kérosène par 100 kilomètres et par passager.  Son pouvoir calorifique n'est "
-"pas très différent ce celui de l'essence de nos voitures, mettons 34 MJ par "
-"litre (43 MJ/kg pour densité de 0.8).  La différence avec la voiture, c'est "
-"qu'on fait beaucoup plus de kilomètres en avion.  Je fais environ un voyage "
-"intercontinental (20 000 km) et quatre voyages domestiques entre Okinawa et "
-"Tôkyô (4 × 2000 km).  Donc environ 840 litres par an, donc 2 400 MJ."
-msgstr ""
-
-#. type: Bullet: ' - '
-msgid ""
-"Ma part dans la facture télécoms familiale, internet plus téléphonie mobile, "
-"est d'environ 5000 yens.  Je n'ai aucune idée précise de la quantité "
+"Ma part dans la facture **télécoms** familiale, internet plus téléphonie "
+"mobile, est d'environ 5000 yens.  Je n'ai aucune idée précise de la quantité "
 "d'énergie utilisée.  Mais on peut estimer une limite supérieure: si cet "
 "argent était entièrement dépensé en électricité par les opérateurs, et "
 "qu'ils la payaient au même prix que nous cela ferait environ 160 kWh, ou "
@@ -125,19 +152,44 @@ msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Je consomme entre 6 et 7 mètres cubes d'eau par mois pour un prix d'environ "
-"1000 yens, qui include la production et le retraitement.  Un rapport de "
-"l'observatoire de l'eau de la Seine-et-Marne sur les enjeux énergétiques de "
-"l'eau potable et de l'assainissement, de 2016, estime entre 1.6 et 16 % la "
-"part énergétique de la facture d'eau.  En extrapolant avec ma facture "
-"d'électricité, 100 yens font environ 11 MJ.  L'eau est précieuse et les "
-"effets de son gaspillage sont différents de ceux du gaspillage de l'énergie."
+"Je consomme entre 6 et 7 mètres cubes d'**eau** par mois pour un prix "
+"d'environ 1000 yens, qui include la production et le retraitement.  Un "
+"rapport de l'observatoire de l'eau de la Seine-et-Marne sur les enjeux "
+"énergétiques de l'eau potable et de l'assainissement, de 2016, estime entre "
+"1.6 et 16 % la part énergétique de la facture d'eau.  En extrapolant avec ma "
+"facture d'électricité, 100 yens font environ 11 MJ.  L'eau est précieuse et "
+"les effets de son gaspillage sont différents de ceux du gaspillage de "
+"l'énergie."
+msgstr ""
+
+#. type: Title ##
+#, no-wrap
+msgid "La vie quotidienne"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Je n'ai aucune idée précise de la quantité d'énergie que j'ingère chaque "
+"jour.  Mon pèse-personne, calibré pour une masse corporelle japonaise, me "
+"recommande de manger 1700 kilocalories par jour.  C'est gross-modo mon "
+"**métabolisme** de base d'après les chiffres résumés par l'ANSES dans on "
+"avis suivant la saisine 2012-SA-0103.  Dans ce même document, les besoins "
+"d'un homme de mon age « modérément actif » sont un peu au dessus de 2500 "
+"kcal par jour.  Une calorie vaut environ 4.2 Joules.  10 000 kJoules valent "
+"un peu moins de 2400 kcal.  En approximant ça à ma consommation quotidienne, "
+"j'en déduis que je consomme environ 300 MJ par mois.  Reste à savoir: "
+"l'énergie dépensée pour me fournir cette nourriture…"
+msgstr ""
+
+#. type: Title ##
+#, no-wrap
+msgid "Le travail"
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Je travaille sur un campus qui consomme 100 000 000 kWh / an, c'est à dire "
-"360 000 000 MJ / an ou 360 TJ / an.  Cela inclut la consommation des "
+"Je **travaille** sur un campus qui consomme 100 000 000 kWh / an, c'est à "
+"dire 360 000 000 MJ / an ou 360 TJ / an.  Cela inclut la consommation des "
 "résidences de quelques centaines d'étudiants et de chercheurs.  Imaginons "
 "qu'une personne consomme 10 000 kWh par an, c'est à dire 36 GJ, ce qui "
 "serait plus que la moyenne au Japon ou en Amérique, on voit que la "
@@ -147,3 +199,65 @@ msgid ""
 "probablement un peu surestimé.  En comparaison, je consomme 800 MJ * 12 ~= "

(Diff truncated)
Réorganisation et ajouts.
diff --git a/Joules.md b/Joules.md
index 29ec81ac..8194cf9e 100644
--- a/Joules.md
+++ b/Joules.md
@@ -13,16 +13,18 @@ l'unité du Système International, pour mieux comparer.  Je vais poster chacun
 de mes calculs avec le tag [#Joules](https://framapiaf.org/tags/Joules) sur
 Framapiaf.
 
+## Les transports
+
  - « _L'énergie contenue dans l'essence est d'environ 33 600 000 joules par
    litre, ou 46 700 000 joules par kilogramme (de pouvoir calorifique
    supérieur)._ » ([Wikipedia](https://fr.wikipedia.org/wiki/Essence_(hydrocarbure)).
    Reste à savoir: l'énergie consommée pour produire ce litre d'essence…
 
- - Je roule 30 kilomètres par jour pour aller travailler, environ 20 jours par
-   mois, avec une voiture parcourant 25 kilomètres par litre, ça fait 24 litres
-   par mois, donc environ 800 MJ par mois.
+ - Je roule en **voiture** 30 kilomètres par jour pour aller travailler,
+   environ 20 jours par mois.  Avec une voiture hybride parcourant 25
+   kilomètres par litre, ça fait 24 litres par mois, donc environ 800 MJ par mois.
 
- - Pour parcourir un kilomètre en train je dois dépenser entre 10 et 20 yens.
+ - Pour parcourir un kilomètre en **train** je dois dépenser entre 10 et 20 yens.
    Çela me côute environ autant en essence quand je pends la voiture, mais
    c'est sans compter la rentabilisation de la voiture et les taxes sur la
    propriété et le contrôle technique (et pour d'autres, le prix du parking).
@@ -30,22 +32,29 @@ Framapiaf.
    dire environ 825 kJ par km, ou 825 J / m.  C'est très proche des chiffres
    annoncés par Die Bahn pour les transports par train régionaux en Allemagne.
 
-https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/progress-in-climate-protection/energy-efficiency-increased/
+   https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/progress-in-climate-protection/energy-efficiency-increased/
 
- - Je n'ai aucune idée précise de la quantité d'énergie que j'ingère chaque jour.
-   Mon pèse-personne, calibré pour une masse corporelle japonaise, me recommande de
-   manger 1700 kilocalories par jour.  C'est gross-modo mon métabolisme de base
-   d'après les chiffres résumés par l'ANSES dans on avis suivant la saisine
-   2012-SA-0103.  Dans ce même document, les besoins d'un homme de mon age
-   « modérément actif » sont un peu au dessus de 2500 kcal par jour.  Une calorie
-   vaut environ 4.2 Joules.  10 000 kJoules valent un peu moins de 2400 kcal.
-   En approximant ça à ma consommation quotidienne, j'en déduis que je consomme
-   environ 300 MJ par mois.  Reste à savoir: l'énergie dépensée pour me fournir
-   cette nourriture…
+ - On estime la consommation des **avions** modernes entre 2 et 4 litres de
+   kérosène par 100 kilomètres et par passager.  Son pouvoir calorifique n'est
+   pas très différent ce celui de l'essence de nos voitures, mettons 34 MJ par
+   litre (43 MJ/kg pour densité de 0.8).  La différence avec la voiture, c'est
+   qu'on fait beaucoup plus de kilomètres en avion.  Je fais environ un voyage
+   intercontinental (20 000 km) et quatre voyages domestiques entre Okinawa et
+   Tôkyô (4 × 2000 km).  Donc environ 840 litres par an, donc 2 400 MJ.
+
+ - Article intéressant comparant la dépense énergétique de chaque moyen de
+   transport.  La dépense du corps humain est estmiée via la consommation
+   d'oxygène, bien étudiée.  Le rendement musculaire n'est pas très haut : les
+   trois quarts de l'énergie dépensée se dissipent en chaleur, et le reste est
+   disponible pour le mouvement.
+   
+   http://www.economiematin.fr/news-consommation-transports-industrie-services-energie
+
+## Le logement
 
  - Pour nous chauffer et nous rafraîchir, pour utiliser nos appareils
    électro-ménagers, et nos médias à écrans, nous consommons environ 300 kWh
-   d'électricité par mois.  Mettons que ça fait 100 pour moi.  Une puissance de
+   d'**électricité** par mois.  Mettons que ça fait 100 pour moi.  Une puissance de
    1 Watt, c'est 1 Joule par seconde.  Donc 100 kWh font 360 MJ.  Une grande
    partie de cette énergie provient de centrales à charbon et il faut de l'énergie
    pour l'extraire et le transporter.  Une partie de l'électricité produite se
@@ -53,7 +62,7 @@ https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/pr
    supérieure à ce que m'annonce le compteur.
 
  - Pour cuisiner, chauffer notre eau de lavage, et sécher nos habits, nous
-   utilisons environ 12 mètres cube de gaz par mois, donc 4 pour ma part.
+   utilisons environ 12 mètres cube de **gaz** par mois, donc 4 pour ma part.
    Il existe différents types de gaz domestique, et une partie de l'énergie
    dégagée par la combustion se perd par l'émission de vapeur d'eau.  Les chiffres
    donnés sur Wikipédia sont pour une température ambiante de zéro degrés, ce
@@ -63,15 +72,7 @@ https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/pr
    sais pas combien d'énergie supplémentaire il faut dépenser pour me fournir
    ce gaz.
 
- - On estime la consommation des avions modernes entre 2 et 4 litres de
-   kérosène par 100 kilomètres et par passager.  Son pouvoir calorifique n'est
-   pas très différent ce celui de l'essence de nos voitures, mettons 34 MJ par
-   litre (43 MJ/kg pour densité de 0.8).  La différence avec la voiture, c'est
-   qu'on fait beaucoup plus de kilomètres en avion.  Je fais environ un voyage
-   intercontinental (20 000 km) et quatre voyages domestiques entre Okinawa et
-   Tôkyô (4 × 2000 km).  Donc environ 840 litres par an, donc 2 400 MJ.
-
- - Ma part dans la facture télécoms familiale, internet plus téléphonie
+ - Ma part dans la facture **télécoms** familiale, internet plus téléphonie
    mobile, est d'environ 5000 yens.  Je n'ai aucune idée précise de la quantité
    d'énergie utilisée.  Mais on peut estimer une limite supérieure: si cet
    argent était entièrement dépensé en électricité par les opérateurs, et qu'ils
@@ -81,7 +82,7 @@ https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/pr
    entendu, ces chiffres ne tiennent pas compte du fait que côté centres de
    données qui nous fournissent le « contenu », la dépense énergétique est énorme.
 
- - Je consomme entre 6 et 7 mètres cubes d'eau par mois pour un prix d'environ
+ - Je consomme entre 6 et 7 mètres cubes d'**eau** par mois pour un prix d'environ
    1000 yens, qui include la production et le retraitement.  Un rapport de
    l'observatoire de l'eau de la Seine-et-Marne sur les enjeux énergétiques
    de l'eau potable et de l'assainissement, de 2016, estime entre 1.6 et 16 %
@@ -89,7 +90,22 @@ https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/pr
    d'électricité, 100 yens font environ 11 MJ.  L'eau est précieuse et les effets
    de son gaspillage sont différents de ceux du gaspillage de l'énergie.
 
- - Je travaille sur un campus qui consomme 100 000 000 kWh / an, c'est à dire
+## La vie quotidienne
+
+ - Je n'ai aucune idée précise de la quantité d'énergie que j'ingère chaque jour.
+   Mon pèse-personne, calibré pour une masse corporelle japonaise, me recommande de
+   manger 1700 kilocalories par jour.  C'est gross-modo mon **métabolisme** de base
+   d'après les chiffres résumés par l'ANSES dans on avis suivant la saisine
+   2012-SA-0103.  Dans ce même document, les besoins d'un homme de mon age
+   « modérément actif » sont un peu au dessus de 2500 kcal par jour.  Une calorie
+   vaut environ 4.2 Joules.  10 000 kJoules valent un peu moins de 2400 kcal.
+   En approximant ça à ma consommation quotidienne, j'en déduis que je consomme
+   environ 300 MJ par mois.  Reste à savoir: l'énergie dépensée pour me fournir
+   cette nourriture…
+
+## Le travail
+
+ - Je **travaille** sur un campus qui consomme 100 000 000 kWh / an, c'est à dire
    360 000 000 MJ / an ou 360 TJ / an.  Cela inclut la consommation des
    résidences de quelques centaines d'étudiants et de chercheurs.  Imaginons
    qu'une personne consomme 10 000 kWh par an, c'est à dire 36 GJ, ce qui serait
@@ -100,3 +116,33 @@ https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/pr
    surestimé.  En comparaison, je consomme 800 MJ * 12 ~= 10 GJ d'énergie en
    carburant pour aller travailler.
 
+## Le plastique
+
+ - Le **plastique** en général: http://large.stanford.edu/courses/2010/ph240/hamman1/
+
+La plupart du plastique est produit à partir de pétrole ou de gaz.  L'énergie
+de combusion du polyéthylène est d'environ 45 MJ / kg.  En 2008, le monde a
+produit environ 80 Tg de plastique.
+
+Le coût énergétique de la synthèse et manufacture du plastique est
+estimé à 1/2 ou 1/3 du coût total. (à vérifier)
+
+Le coût énergétique de la synthèse et manufacture du plastique est
+estimé à 18 MJ / kg de plastique.
+
+le vin
+
+Aller au travail en vélo
+
+une vie plus bourgeoise ?  Une vie plus maigre ?
+
+Consommation annuelle de pétrole (4.2 10e20 J)/ Énergie envoyée par le soleil: 6,2 × 1020 J 	l'énergie totale du Soleil qui atteint la Terre en une heure. (https://fr.wikipedia.org/wiki/Ordres_de_grandeur_d%27%C3%A9nergie)
+
+L'énergie cinétique d'un 1kg de matière à la vitesse de libération (7.9 km/s)
+est d'un peu plus de 30 MJ.  Il faut brûler un litre d'essence pour dégager
+cette énergie.  Malheureusement, comme on n'a pas encore inventé mieux, il faut
+aussi transporter l'essence et le moteur, ce qui alourdit considérablement
+l'ensemble, au point de doubler le poids et donc l'énergie demandée, et donc le
+poids, et donc l'énergie demandée, etc.  Note: la différence d'énergie
+potentielle dans le champs de gravité terrestre est largement inférieure à la
+quantité d'énergie cinétique nécessaire.

Train
diff --git a/Joules.md b/Joules.md
index cecfb116..29ec81ac 100644
--- a/Joules.md
+++ b/Joules.md
@@ -22,6 +22,16 @@ Framapiaf.
    mois, avec une voiture parcourant 25 kilomètres par litre, ça fait 24 litres
    par mois, donc environ 800 MJ par mois.
 
+ - Pour parcourir un kilomètre en train je dois dépenser entre 10 et 20 yens.
+   Çela me côute environ autant en essence quand je pends la voiture, mais
+   c'est sans compter la rentabilisation de la voiture et les taxes sur la
+   propriété et le contrôle technique (et pour d'autres, le prix du parking).
+   Mes trajets en voiture consomment (voir plus haut) 1/25 L par km, c'est à
+   dire environ 825 kJ par km, ou 825 J / m.  C'est très proche des chiffres
+   annoncés par Die Bahn pour les transports par train régionaux en Allemagne.
+
+https://ibir.deutschebahn.com/ib2018/en/group-management-report/environmental/progress-in-climate-protection/energy-efficiency-increased/
+
  - Je n'ai aucune idée précise de la quantité d'énergie que j'ingère chaque jour.
    Mon pèse-personne, calibré pour une masse corporelle japonaise, me recommande de
    manger 1700 kilocalories par jour.  C'est gross-modo mon métabolisme de base

APEX2 / APE2
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 475eb22c..3791499b 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -276,6 +276,8 @@ Genes and pathways
    ([[Niimura, 2009|biblio/20333175]]).
  - Most members of retinoic acid pathway gene network (biosynthesis, signalling
    and degradation) are lost in _O. dioica_ ([[Martí-Solans et al., 2016|biblio/27406791]]).
+ - APEX2 (encoding for the APE2 protein involved in base excision repair and microhomology
+   mediated end-joining (MMEJ) was not found, but APEX1 was found ([[Denoeud et al., 2010|biblio/21097902]]).
  - The entire machinery required for performing classical NHEJ repair of DSB (which is
    conserved from yeast to mammals) is undetectable ([[Denoeud et al., 2010|biblio/21097902]]).
    An alternative or microhomology (MH)-driven end joining pathway is active

larvacea
diff --git a/biblio/biostor-236874.mdwn b/biblio/biostor-236874.mdwn
new file mode 100644
index 00000000..a001e3bb
--- /dev/null
+++ b/biblio/biostor-236874.mdwn
@@ -0,0 +1,21 @@
+[[!meta title="A revised classification of the Tunicata, with definitions of the orders, sub-orders, families, sub-families and genera, and analytical keys to the species"]]
+[[!tag Oikopleura]]
+
+William Abbott Herdman
+
+Journal of The Linnean Society of London, Zoology, vol 23, 558-652, Feb 5th 1891.  ark:/13960/t5q898f4x, biostor-236874.
+
+A revised classification of the Tunicata, with definitions of the orders, sub-orders, families, sub-families and genera, and analytical keys to the species
+
+Defines:
+
+ - Order Larvacea, Herdman, 1882
+   - Family Appendiculariidae, Bronn, 1862
+     - Genus Appendicularia (Chamisso 1821), Fol, 1874
+     - Genus Oikopoleura (Mertens 1831), Fol, 1872
+             synonym: Vexillaria, J. Müller
+     - Genus Stegosoma, Chun 1888
+     - Genus Fritillaria (Q. & G. 1833), Fol, 1872
+             synonym: Eurycercus, Busch
+     - Genus Kowalvskia, Fol, 1872
+             Herdman speculates that it may be a Family.

outliers
diff --git a/biblio/22925495.mdwn b/biblio/22925495.mdwn
new file mode 100644
index 00000000..6669e60c
--- /dev/null
+++ b/biblio/22925495.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Gene functionalities and genome structure in Bathycoccus prasinos reflect cellular specializations at the base of the green lineage."]]
+[[!tag genome chromosome repeats]]
+
+Moreau H, Verhelst B, Couloux A, Derelle E, Rombauts S, Grimsley N, Van Bel M, Poulain J, Katinka M, Hohmann-Marriott MF, Piganeau G, Rouzé P, Da Silva C, Wincker P, Van de Peer Y, Vandepoele K.
+
+Genome Biol. 2012 Aug 24;13(8):R74. doi:10.1186/gb-2012-13-8-r74
+
+Gene functionalities and genome structure in Bathycoccus prasinos reflect cellular specializations at the base of the green lineage.
+
+[[!pmid 22925495 desc="7,847 genes, no transposons.  Two “outlier” chromosomes are found with lower GC content, like in _Ostreococcus_ and _Micromonas_.  Genes in the big outlier chromosomes have a high number of small AT-rich introns with no apparent conserved motifs."]]
diff --git a/tags/chromosome.mdwn b/tags/chromosome.mdwn
index 288710cd..ac6a4887 100644
--- a/tags/chromosome.mdwn
+++ b/tags/chromosome.mdwn
@@ -6,7 +6,9 @@ _in progressss_
 
 Two (out of 20) chromosomes in the genome of the unicellular green alga
 _Ostreococcus tauri_ have a markedly different GC and transposable element
-content compared to the others. [[Derelle et al., 2003|biblio/16868079]].
+content compared to the others. [[Derelle and coll., 2003|biblio/16868079]].
+The same phenomeon has then been reported in _Bathycoccus prasinos_ ([[Moreau
+and coll., 2012|biblio/22925495]]) and other species.
 
 Chr4 in _Polypedilum vanderplanki_ has lower GC content than the others
 ([[Yoshida et al., 2022|biblio/35387384]]), and it has been speculated that it

creating tag page tags/mangrove
diff --git a/tags/mangrove.mdwn b/tags/mangrove.mdwn
new file mode 100644
index 00000000..e47229d1
--- /dev/null
+++ b/tags/mangrove.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged mangrove"]]
+
+[[!inline pages="tagged(mangrove)" actions="no" archive="yes"
+feedshow=10]]

WGD
diff --git a/biblio/31258950.mdwn b/biblio/31258950.mdwn
new file mode 100644
index 00000000..ce8c747b
--- /dev/null
+++ b/biblio/31258950.mdwn
@@ -0,0 +1,13 @@
+[[!meta title="The origin, diversification and adaptation of a major mangrove clade (Rhizophoreae) revealed by whole-genome sequencing."]]
+[[!tag mangrove genome]]
+
+Xu, S., He, Z., Zhang, Z., Guo, Z., Guo, W., Lyu, H., Li, J., Yang, M., Du, Z., Huang, Y., Zhou, R., Zhong, C., Boufford, D. E., Lerdau, M., Wu, C. I., Duke, N. C., International Mangrove Consortium, & Shi, S.
+
+National science review, 4(5), 721–734, 2017. doi:10.1093/nsr/nwx065
+
+The origin, diversification and adaptation of a major mangrove clade (Rhizophoreae) revealed by whole-genome sequencing 
+
+[[!pmid 31258950 desc="Analysis of the genome sequence of _Rhizophora
+apiculata_ suggests a whole-genome duplication.  dN/dS analysis of the
+paralogues and comparisons to outgroups suggest that the duplication took place
+in the ancestors of _Rhizophoreae_."]]

PBS
diff --git a/biblio/25828383.mdwn b/biblio/25828383.mdwn
new file mode 100644
index 00000000..bbffdb84
--- /dev/null
+++ b/biblio/25828383.mdwn
@@ -0,0 +1,13 @@
+[[!meta title="Printing 2-dimentional droplet array for single-cell reverse transcription quantitative PCR assay with a microfluidic robot"]]
+[[!tag PCR]]
+
+Zhu Y, Zhang YX, Liu WW, Ma Y, Fang Q, Yao B.
+
+Sci Rep. 2015 Apr 1;5:9551. doi:10.1038/srep09551
+
+Printing 2-dimentional droplet array for single-cell reverse transcription quantitative PCR assay with a microfluidic robot
+
+[[!pmid 25828383 desc="For the amplification of a miRNA, the authors reported a
+98.03% efficiency in standard conditions.  It dropped to 90.94% with 0.5 mM
+49.99% PBS, 86.96% with 2 mM, 80.47% with 10 mM (1×), and 49.99% with 50 mM.
+The PCR was done in TaqMan universal PCR master mix."]]

Fungi
diff --git a/tags/yeast.mdwn b/tags/yeast.mdwn
index da1870c0..2e16ab37 100644
--- a/tags/yeast.mdwn
+++ b/tags/yeast.mdwn
@@ -1,4 +1,6 @@
 [[!meta title="pages tagged yeast"]]
 
+# Yeast and other unicellular fungi
+
 [[!inline pages="tagged(yeast)" actions="no" archive="yes"
 feedshow=10]]

Café hier
diff --git a/biblio/30618839.mdwn b/biblio/30618839.mdwn
new file mode 100644
index 00000000..bec42c55
--- /dev/null
+++ b/biblio/30618839.mdwn
@@ -0,0 +1,17 @@
+[[!meta title="A Phylogenomic Framework and Divergence History of Cephalochordata Amphioxus."]]
+[[!tag phylogeny]]
+
+Front Physiol. 2018 Dec 18;9:1833. doi:10.3389/fphys.2018.01833
+
+Zhang QL, Zhang GL, Yuan ML, Dong ZX, Li HW, Guo J, Wang F, Deng XY, Chen JY, Lin LB.
+
+A Phylogenomic Framework and Divergence History of Cephalochordata Amphioxus. 
+
+[[!pmid 30618839 desc="Supports “a phylogeny of [(B. belcheri + B. japonicum) +
+(B. lanceolatum + B. floridae) + Asymmetron lucayanum] in amphioxus”.
+Estimates that “the most recent common ancestor of the living amphioxus species
+(the divergence time between _Asymmetron_ and _Branchiostoma_) was estimated to
+be ∼104 Mya [23.74—211.63]; the early splits within each main clade occurred at
+∼87 Mya [41.02—162.19]; _B. lanceolatum_ diverged from _B. floridae_ at ∼72 Mya
+[31.93—149.68], and the divergence time between _B. belcheri_ and _B.
+japonicum_ was ∼61 Mya [10.21—136.41]”."]]

Fritillaria
diff --git a/biblio/36307829.mdwn b/biblio/36307829.mdwn
index 4598663c..18eb634f 100644
--- a/biblio/36307829.mdwn
+++ b/biblio/36307829.mdwn
@@ -8,9 +8,10 @@ Front Zool. 2022 Oct 28;19(1):26. doi:10.1186/s12983-022-00471-y
 Laboratory study of Fritillaria lifecycle reveals key morphogenetic events leading to genus-specific anatomy.
 
 [[!pmid 36307829 desc="Possibly parasited by a Syndiniale from the genus
-_Sphaeripara_ and _Oodinium_.  Fed with _Micromonas_, _Phaeocystis_ and
-_Synechococcus_ in culture. 4 chromosomes are visible in oocytes by DAPI
-staining.  Self-fertilization was never observed.  In vitro fertilized oocytes
-frequently aborted development when left in vessels without water flow.
-Embryos hatch between 4 and 6 h at 15 °C. Cellulose-producing cells identified
-by staining with a carbohydrate-binding-module (CBM)."]]
+_Sphaeripara_ and _Oodinium_.  Fed with _Micromonas pusilla_ or _Synechococcus
+sp_ in culture at a density between 2 and 8 × 10<sup>7</sup> depending on the
+developmental stage. 4 chromosomes are visible in oocytes by DAPI staining.
+Self-fertilization was never observed.  In vitro fertilized oocytes frequently
+aborted development when left in vessels without water flow.  Embryos hatch
+between 4 and 6 h at 15 °C. Cellulose-producing cells identified by staining
+with a carbohydrate-binding-module (CBM)."]]
diff --git a/tags/Fritillaria.mdwn b/tags/Fritillaria.mdwn
index ed9d0dc3..84d424a9 100644
--- a/tags/Fritillaria.mdwn
+++ b/tags/Fritillaria.mdwn
@@ -4,6 +4,12 @@ _in progress_
 
  - _Fritillaria borealis_ can swim with its house deflated ([[Flood, 2003|biblio/10.1007_s00227-003-1075-y]]).
 
+ - Fed in culture with _Micromonas pusilla_ or _Synechococcus sp_ in culture at
+   a density between 2 and 8 × 10<sup>7</sup> depending on the developmental
+   stage ([[Henriet, Asajord and Chourrout, 2022|biblio/36307829]]).
+
+ - Karyotype of 4 chromosomes ([[Henriet, Asajord and Chourrout, 2022|biblio/36307829]]).
+
 # Found in:
 
 ## Antarctica

creating tag page tags/clock
diff --git a/tags/clock.mdwn b/tags/clock.mdwn
new file mode 100644
index 00000000..50f3f75d
--- /dev/null
+++ b/tags/clock.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged clock"]]
+
+[[!inline pages="tagged(clock)" actions="no" archive="yes"
+feedshow=10]]

Details on the molecular clock analysis.
diff --git a/biblio/36867684.mdwn b/biblio/36867684.mdwn
index 76e76691..3a99120f 100644
--- a/biblio/36867684.mdwn
+++ b/biblio/36867684.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. "]]
-[[!tag genome]]
+[[!tag genome clock]]
 
 Huang Z, Xu L, Cai C, Zhou Y, Liu J, Xu Z, Zhu Z, Kang W, Cen W, Pei S, Chen D, Shi C, Wu X, Huang Y, Xu C, Yan Y, Yang Y, Xue T, He W, Hu X, Zhang Y, Chen Y, Bi C, He C, Xue L, Xiao S, Yue Z, Jiang Y, Yu JK, Jarvis ED, Li G, Lin G, Zhang Q, Zhou Q. 
 
@@ -7,4 +7,28 @@ Proc Natl Acad Sci U S A. 2023 Mar 7;120(10):e2201504120. doi:10.1073/pnas.22015
 
 Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. 
 
-[[!pmid 36867684 desc="Chromosomal assembly of _Branchiostoma floridae_ (Bf), _Branchiostoma belcheri_ (Bb) and _Branchiostoma japonicum_ (Bj).  ”we devised an interspecific trio sequencing strategy [...] for the F1 hybrids derived from Bf-Bb or Bf-Bj crosses. [...] the hybrids contain two haploid parental genomes that have become too diverged in sequences to form cross-species chimeric assembly”  “The three amphioxus genomes show a highly conserved chromosomal synteny to each other, with  most  chromosomes  showing  a  one-to-one  homologous  relationship except for a few chromosome fusions.”  ”genome-wide heterozygosity [estimated to] range from 3.2 to 4.2%”  ”Based on 3,653 single-copy orthologous genes, we estimated that different chordate lineages diverged about 592.5 Mya, and three amphioxus  species  diverged  about  99.9  Mya”.  “Using whole-genome resequencing data of between 10 and 48 individuals per sex per species, we identified the sexually differentiated regions (SDR) that harbor [...] excessive  female heterozygotes, and are not shared between the three amphioxus species.  In particular, the SDR of Bf is located on Chr16 and harbors 194 genes; and those of Bj and Bb are located at two different genomic loci of Chr3, harboring 35 genes and one gene with unknown function respectively. Bf Chr16 is not homologous with Chr3 of Bj or Bb.”"]]
+[[!pmid 36867684 desc="Chromosomal assembly of _Branchiostoma floridae_ (Bf),
+_Branchiostoma belcheri_ (Bb) and _Branchiostoma japonicum_ (Bj).  ”we devised
+an interspecific trio sequencing strategy [...] for the F1 hybrids derived from
+Bf-Bb or Bf-Bj crosses. [...] the hybrids contain two haploid parental genomes
+that have become too diverged in sequences to form cross-species chimeric
+assembly”  “The three amphioxus genomes show a highly conserved chromosomal
+synteny to each other, with most chromosomes showing a one-to-one homologous
+relationship except for a few chromosome fusions.”  ”genome-wide heterozygosity
+[estimated to] range from 3.2 to 4.2%”  ”Based on 3,653 single-copy orthologous
+genes, we estimated that different chordate lineages diverged about 592.5 Mya,
+and three amphioxus species diverged about 99.9 Mya”.  “Using whole-genome
+resequencing data of between 10 and 48 individuals per sex per species, we
+identified the sexually differentiated regions (SDR) that harbor [...]
+excessive female heterozygotes, and are not shared between the three amphioxus
+species.  In particular, the SDR of Bf is located on Chr16 and harbors 194
+genes; and those of Bj and Bb are located at two different genomic loci of
+Chr3, harboring 35 genes and one gene with unknown function respectively. Bf
+Chr16 is not homologous with Chr3 of Bj or Bb.”"]]
+
+Mean and confidence interval for divergence times given in Table S2:
+
+ - _B. belcheri_ and _B. japonicum_: 59.3 [12.2, 132.0].
+ - all _Branchiostoma_: 99.9 [22.1, 211.7].
+ - all chordates: 592.5 [530.8, 640.3].  Prior: [514, 636.1].
+ - Euarchontoglires: 116.1 [72.1, 163.1].  Prior: [65.6, 164.6].

Résolu
diff --git a/dh.txt b/dh.txt
deleted file mode 100644
index e02faf22..00000000
--- a/dh.txt
+++ /dev/null
@@ -1,21 +0,0 @@
------BEGIN PGP SIGNED MESSAGE-----
-Hash: SHA512
-
-Hello DreahHost Customer Service I am Charles Plessy and we are discussing in the email thread 226139527 
-
------BEGIN PGP SIGNATURE-----
-
-iQIzBAEBCgAdFiEEc0cUmcxg7Z7ugFlGxb1sjyKV1QIFAmPwFvMACgkQxb1sjyKV
-1QImoRAAob7ZPH9T3/AG6p96RSulnOXfCxEcq7rDc3pFZdgIgsI9e0od0CFIlUtT
-Dnbwycgd+RFOwk+VeEbj0eWAFb4PBEZO1G3X4YEiQDLVv5aNsLEdenNC9+H0x6XT
-NigFIHTILvHwpcdtUfOHnUZe+1vwo8DsZHNrc3FQ3uOrygcDvzzXdmQY1blRpq37
-uCtuhyBHBV5mJL+QQ9109xCaa+m8edZKAxIk2egXndJo+dVX/iSAJ7IOshFQwE+4
-9+8l5gDAHxmk0pB9/Wu5J5IAlX+fZ2bjqSRZK+GvfxNjnoEZyUtWVZ1YF5iKKiq0
-0862YnDBrR9ajRtCVskiZ3ML6ySujbo8gnMYBe36JqyhtZNbEM2DvQtdCzac47Ep
-CSmFR7mYO3vidH8ETqKiMPAErq27lAaYzdq7MWwWaLVxqu/w0b6D7+Npc9d1lx52
-j4Qv693SVLU9GvYxHZZ0HJWSmYIPMR13ldXOR8t/88M7ANQ2tGDYG1cVIqMwl7OH
-88WUrJ8aBnZPwi2lq5Up956wFg7JQqtb/O8S7SS9LumXZJAXC4qOfN2AsDkoxEr/
-aVqtKLNJBPky7kn1o21dbUpilz70jjoU46KFZIe//njwsnebqq+bB/Y4HDlXxBKz
-cX9jlPji2MCqg7LfH/5rJAeCKhtCBXyF4EdyxJsU87TlE+uKikw=
-=WBtm
------END PGP SIGNATURE-----

Café
diff --git a/biblio/36867684.mdwn b/biblio/36867684.mdwn
new file mode 100644
index 00000000..76e76691
--- /dev/null
+++ b/biblio/36867684.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. "]]
+[[!tag genome]]
+
+Huang Z, Xu L, Cai C, Zhou Y, Liu J, Xu Z, Zhu Z, Kang W, Cen W, Pei S, Chen D, Shi C, Wu X, Huang Y, Xu C, Yan Y, Yang Y, Xue T, He W, Hu X, Zhang Y, Chen Y, Bi C, He C, Xue L, Xiao S, Yue Z, Jiang Y, Yu JK, Jarvis ED, Li G, Lin G, Zhang Q, Zhou Q. 
+
+Proc Natl Acad Sci U S A. 2023 Mar 7;120(10):e2201504120. doi:10.1073/pnas.2201504120
+
+Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. 
+
+[[!pmid 36867684 desc="Chromosomal assembly of _Branchiostoma floridae_ (Bf), _Branchiostoma belcheri_ (Bb) and _Branchiostoma japonicum_ (Bj).  ”we devised an interspecific trio sequencing strategy [...] for the F1 hybrids derived from Bf-Bb or Bf-Bj crosses. [...] the hybrids contain two haploid parental genomes that have become too diverged in sequences to form cross-species chimeric assembly”  “The three amphioxus genomes show a highly conserved chromosomal synteny to each other, with  most  chromosomes  showing  a  one-to-one  homologous  relationship except for a few chromosome fusions.”  ”genome-wide heterozygosity [estimated to] range from 3.2 to 4.2%”  ”Based on 3,653 single-copy orthologous genes, we estimated that different chordate lineages diverged about 592.5 Mya, and three amphioxus  species  diverged  about  99.9  Mya”.  “Using whole-genome resequencing data of between 10 and 48 individuals per sex per species, we identified the sexually differentiated regions (SDR) that harbor [...] excessive  female heterozygotes, and are not shared between the three amphioxus species.  In particular, the SDR of Bf is located on Chr16 and harbors 194 genes; and those of Bj and Bb are located at two different genomic loci of Chr3, harboring 35 genes and one gene with unknown function respectively. Bf Chr16 is not homologous with Chr3 of Bj or Bb.”"]]

updated PO files
diff --git "a/Debian/debi\303\242neries/dreamhost.en.po" "b/Debian/debi\303\242neries/dreamhost.en.po"
index 414ac52e..101ff943 100644
--- "a/Debian/debi\303\242neries/dreamhost.en.po"
+++ "b/Debian/debi\303\242neries/dreamhost.en.po"
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2023-03-09 13:38+0000\n"
+"POT-Creation-Date: 2023-03-10 03:07+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -36,6 +36,15 @@ msgstr ""
 msgid "[[!meta title=\"If you work at Dreamhost, can you help us?\"]]\n"
 msgstr ""
 
+#. type: Plain text
+#, no-wrap
+msgid ""
+"***Update: thanks to the very kind involvment of the widow of our wemaster, we\n"
+"could provide enough private information to Dreamhost, who finally accepted to\n"
+"reset the password and the MFA.  We have recovered evrything!  Many thanks to\n"
+"everybody who helped us!***\n"
+msgstr ""
+
 #. type: Plain text
 msgid ""
 "Due to tragic circumstances, one association that I am part of, "

Bonne nouvelle.
diff --git "a/Debian/debi\303\242neries/dreamhost.mdwn" "b/Debian/debi\303\242neries/dreamhost.mdwn"
index f4e52e6d..bddf20cc 100644
--- "a/Debian/debi\303\242neries/dreamhost.mdwn"
+++ "b/Debian/debi\303\242neries/dreamhost.mdwn"
@@ -4,6 +4,11 @@
 
 [[!meta title="If you work at Dreamhost, can you help us?"]]
 
+***Update: thanks to the very kind involvment of the widow of our wemaster, we
+could provide enough private information to Dreamhost, who finally accepted to
+reset the password and the MFA.  We have recovered evrything!  Many thanks to
+everybody who helped us!***
+
 Due to tragic circumstances, one association that I am part of,
 [Sciencescope](https://www.sciencescope.org) got locked out of its account at
 Dreamhost.  Locked out, we can not pay the annual bill.  Dreamhost contacted us

updated PO files
diff --git "a/Debian/debi\303\242neries/dreamhost.en.po" "b/Debian/debi\303\242neries/dreamhost.en.po"
new file mode 100644
index 00000000..414ac52e
--- /dev/null
+++ "b/Debian/debi\303\242neries/dreamhost.en.po"
@@ -0,0 +1,68 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2023-03-09 13:38+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Thu, 09 Mar 2023 22:35:00 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Thu, 09 Mar 2023 22:35:00 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"If you work at Dreamhost, can you help us?\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Due to tragic circumstances, one association that I am part of, "
+"[Sciencescope](https://www.sciencescope.org) got locked out of its account "
+"at Dreamhost.  Locked out, we can not pay the annual bill.  Dreamhost "
+"contacted us about the payment, but will not let us recover the access to "
+"our account in order to pay.  So they will soon close the account.  Our "
+"website, mailing lists and archives, will be erased.  We provided plenty of "
+"evidence that we are not scammers and that we are the legitimate owners of "
+"the account, but reviewing it is above the pay grade of the custommer "
+"support (I don't blame them) and I could not convince them to let somebody "
+"higher have a look at our case."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"If you work at Dreamhost and want to keep us as custommers instead of "
+"kicking us like that, please ask the support service in charge of ticket "
+"225948648 to send the recovery URL to the _secondary_ email adddresses (the "
+"ones you used to contact us about the bill!) in addition to the _primary_ "
+"one (which nobody will read anymore).  You can encrypt it for my Debian "
+"Developer key 73471499CC60ED9EEE805946C5BD6C8F2295D502 if you worry it gets "
+"in wrong hands.  If you still have doubts I am available for calls any time."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"If you know somebody working at Dreamhost can you pass them the message? "
+"This would be a big, big, relief for our non-profit association."
+msgstr ""

help
diff --git "a/Debian/debi\303\242neries/dreamhost.mdwn" "b/Debian/debi\303\242neries/dreamhost.mdwn"
new file mode 100644
index 00000000..f4e52e6d
--- /dev/null
+++ "b/Debian/debi\303\242neries/dreamhost.mdwn"
@@ -0,0 +1,26 @@
+[[!meta date="Thu, 09 Mar 2023 22:35:00 +0900"]]
+[[!meta updated="Thu, 09 Mar 2023 22:35:00 +0900"]]
+[[!tag Debian]]
+
+[[!meta title="If you work at Dreamhost, can you help us?"]]
+
+Due to tragic circumstances, one association that I am part of,
+[Sciencescope](https://www.sciencescope.org) got locked out of its account at
+Dreamhost.  Locked out, we can not pay the annual bill.  Dreamhost contacted us
+about the payment, but will not let us recover the access to our account in
+order to pay.  So they will soon close the account.  Our website, mailing lists
+and archives, will be erased.  We provided plenty of evidence that we are not
+scammers and that we are the legitimate owners of the account, but reviewing it
+is above the pay grade of the custommer support (I don't blame them) and I
+could not convince them to let somebody higher have a look at our case.
+
+If you work at Dreamhost and want to keep us as custommers instead of kicking
+us like that, please ask the support service in charge of ticket 225948648 to
+send the recovery URL to the _secondary_ email adddresses (the ones you used to
+contact us about the bill!) in addition to the _primary_ one (which nobody will
+read anymore).  You can encrypt it for my Debian Developer key
+73471499CC60ED9EEE805946C5BD6C8F2295D502 if you worry it gets in wrong hands.
+If you still have doubts I am available for calls any time.
+
+If you know somebody working at Dreamhost can you pass them the message?
+This would be a big, big, relief for our non-profit association.

Hello DreamHost
diff --git a/dh.txt b/dh.txt
new file mode 100644
index 00000000..e02faf22
--- /dev/null
+++ b/dh.txt
@@ -0,0 +1,21 @@
+-----BEGIN PGP SIGNED MESSAGE-----
+Hash: SHA512
+
+Hello DreahHost Customer Service I am Charles Plessy and we are discussing in the email thread 226139527 
+
+-----BEGIN PGP SIGNATURE-----
+
+iQIzBAEBCgAdFiEEc0cUmcxg7Z7ugFlGxb1sjyKV1QIFAmPwFvMACgkQxb1sjyKV
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+Dnbwycgd+RFOwk+VeEbj0eWAFb4PBEZO1G3X4YEiQDLVv5aNsLEdenNC9+H0x6XT
+NigFIHTILvHwpcdtUfOHnUZe+1vwo8DsZHNrc3FQ3uOrygcDvzzXdmQY1blRpq37
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+=WBtm
+-----END PGP SIGNATURE-----

Café
diff --git a/biblio/21685081.mdwn b/biblio/21685081.mdwn
new file mode 100644
index 00000000..3a9ffeb7
--- /dev/null
+++ b/biblio/21685081.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="PhyloCSF: a comparative genomics method to distinguish protein coding and non-coding regions."]]
+[[!tag genome alignment]]
+
+Lin MF, Jungreis I, Kellis M.
+
+Bioinformatics. 2011 Jul 1;27(13):i275-82. doi: 10.1093/bioinformatics/btr209
+
+PhyloCSF: a comparative genomics method to distinguish protein coding and non-coding regions.
+
+[[!pmid 21685081 desc="Classifies coding and non-coding regions using multiple genome sequence alignments and the fit with separate codon score matrices for coding an non-coding."]]
diff --git a/tags/alignment.mdwn b/tags/alignment.mdwn
index 89b1ef03..b98b01be 100644
--- a/tags/alignment.mdwn
+++ b/tags/alignment.mdwn
@@ -21,4 +21,8 @@ see [[LAST]] for a detailed bibliography.
    an iterative approach where ancestral genomes are reconstituted using 2-5 pairs
    of in- and out-group comparisons, and then progressively aligned to each other.
 
+## Consumers of multiple genome sequence alignments
+
+ - PhyloCSF ([[Lin, Jungreis and Kellis (2011)|biblio/21685081]]).
+
 [[!inline pages="tagged(alignment)" limit=0]]

Café
diff --git a/biblio/34853330.mdwn b/biblio/34853330.mdwn
new file mode 100644
index 00000000..f7aec4dd
--- /dev/null
+++ b/biblio/34853330.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Metabarcoding analysis of trophic sources and linkages in the plankton community of the Kuroshio and neighboring waters."]]
+[[!tag eDNA]]
+
+Kobari T, Tokumo Y, Sato I, Kume G, Hirai J.
+
+Sci Rep. 2021 Dec 1;11(1):23265 doi:10.1038/s41598-021-02083-8
+
+Metabarcoding analysis of trophic sources and linkages in the plankton community of the Kuroshio and neighboring waters.
+
+[[!pmid 34853330 desc="eDNA analysis of “the diets of 22 higher taxonomic groups, 13 copepod families and 35 species, that dominate in the Kuroshio food web”.  Larvacean DNA was more frequent in predator gut contents than in water samples.  “The 18S rRNA V9 region was amplified using eukaryotic universal primers 1389F and 1510R.”"]]
diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
index 3aa11f94..f284280c 100644
--- a/tags/eDNA.mdwn
+++ b/tags/eDNA.mdwn
@@ -16,6 +16,9 @@
  - Edgar ([[2018|biblio/29506021]] proposes to raise the threshold to 99% or to
    just use sequence variants (also called ZOTUS, Zero-radius OTUs. 
 
+ - Larvacean DNA found more frequently in predator gut than in water samples
+   ([[Kobari and coll., 2021|biblio/34853330]]).  Uses the V9 region 18S primers.
+
 ### Published primers
 
 ```

Details on mechanism.
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 65fbf91b..222faccd 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -51,6 +51,9 @@ Flanking an inversion in _D. buzzati_ between two _Galileo_ elements, the
 exchange of Target-Site Duplication sequences was observed by Cáceres and
 coll., [[(1999|biblio/10411506]]). 
 
+Ectopic recombination of a Galileo element may have caused a recent large-scale
+inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
+
 [[Kent and coll., 2003|biblio/14500911]] reported 2 inversions per Mbp in
 human/mouse comparisons, median length 814. 
 
@@ -60,8 +63,11 @@ _Staggered breaks_ ([[Ranz and coll., 2007|bilbio/17550304]]) caused
 by inverted duplications.  The duplicated sequnces may be coding genes or
 pseudogenes, not just transposable elements.
 
-Ectopic recombination of a Galileo element may have caused a recent large-scale
-inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
+([[Delprat and coll, 2009|biblio/19936241]]) proposed that “Chromosomal
+inversions may be generated by transposons when two ends that are not part of
+the same transposon participate in an aberrant transposition event to a new
+site”.  They give an example where the two transposons are identical copies
+on sister chromatids in G2 phase.
 
 ## Software
 

Reorganise avec du café
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index dc41893b..65fbf91b 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -5,7 +5,9 @@ _in progress_
 Long read sequencing data from 3,622 Icelanders identified a median of 22,636
 SVs per individual (insertios: 13,353; deletions: 9,474) [[Beyter and coll, 2021|biblio/33972781]].
 
-### Inversions
+## Inversions
+
+### Within-population
 
 SNP analysis in >1400 seaweed flies identified known and candidate genomic inversions
 ([[Mérot and coll, 2021|biblio/33963409]]).
@@ -14,6 +16,15 @@ SV analysis of 31 diploid assemblies of _Theobroma cacao_ showed relaxed
 selection and increased genetic load in inversions and other types of variants
 [[Hämälä and coll., 2021|biblio/34408075]].
 
+[[Stefansson and coll, 2005||biblio/15654335]] found an inversion in the human
+genome and calculated that it appeared ~3 million years ago, before the
+speciation of _Homo sapiens_.  Low-copy repeated sequences were found in the
+vicinity.  [[Donelly and coll., 2010|biblio/20116045]] gave a radically lower
+time estimate of 13,600 to 108,400 years, but an ancient origin was again
+supported by [[Steinberg and coll in 2012|biblio/22751100]].
+
+### Between-species
+
 ~1100 small-scale inversions are estimated to have happened between _S.
 cerevisiae_ and _C. albicans_ ([[Seoighe and coll., 2000|biblio/11087826]]).  The authors propose that
 “successive multigene inversions” have “distrupted” the “precise arrangement”
@@ -36,16 +47,6 @@ isochromatid or chromatid, and that this, rather than ectopic exchange between
 inverted repetitive sequences, is the prevalent mechanism for the generation of
 inversions in the melanogaster species group”. 
 
-[[Stefansson and coll, 2005||biblio/15654335]] found an inversion in the human
-genome and calculated that it appeared ~3 million years ago, before the
-speciation of _Homo sapiens_.  Low-copy repeated sequences were found in the
-vicinity.  [[Donelly and coll., 2010|biblio/20116045]] gave a radically lower
-time estimate of 13,600 to 108,400 years, but an ancient origin was again
-supported by [[Steinberg and coll in 2012|biblio/22751100]].
-
-Ectopic recombination of a Galileo element may have caused a recent large-scale
-inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
-
 Flanking an inversion in _D. buzzati_ between two _Galileo_ elements, the
 exchange of Target-Site Duplication sequences was observed by Cáceres and
 coll., [[(1999|biblio/10411506]]). 
@@ -53,7 +54,16 @@ coll., [[(1999|biblio/10411506]]).
 [[Kent and coll., 2003|biblio/14500911]] reported 2 inversions per Mbp in
 human/mouse comparisons, median length 814. 
 
-### Software
+### Mechanism
+
+_Staggered breaks_ ([[Ranz and coll., 2007|bilbio/17550304]]) caused
+by inverted duplications.  The duplicated sequnces may be coding genes or
+pseudogenes, not just transposable elements.
+
+Ectopic recombination of a Galileo element may have caused a recent large-scale
+inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
+
+## Software
 
  - _NanoSV_ ([[Cretu Stancu and coll., 2017|biblio/29109544]]) uses nanopore long
    reads aligned to a reference genome with last-split

Café
diff --git a/biblio/17550304.mdwn b/biblio/17550304.mdwn
new file mode 100644
index 00000000..c208753a
--- /dev/null
+++ b/biblio/17550304.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Principles of genome evolution in the Drosophila melanogaster species group."]]
+[[!tag variants Drosophila]]
+
+Ranz JM, Maurin D, Chan YS, von Grotthuss M, Hillier LW, Roote J, Ashburner M, Bergman CM.
+
+PLoS Biol. 2007 Jun;5(6):e152. doi:10.1371/journal.pbio.0050152
+
+Principles of genome evolution in the Drosophila melanogaster species group.
+
+[[!pmid 17550304 desc="“the breakpoint regions of 59% of the inversions (17/29) are associated with inverted duplications of genes or other nonrepetitive sequences”  “We propose that the presence of inverted duplications associated with inversion breakpoint regions is the result of staggered breaks, either isochromatid or chromatid, and that this, rather than ectopic exchange between inverted repetitive sequences, is the prevalent mechanism for the generation of inversions in the melanogaster species group”  “The overall rate of breakage in the _D. melanogaster_ / _D. yakuba_ lineage is 0.0183/Mb/Myr.”"]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 5bdf6ae5..dc41893b 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -28,6 +28,14 @@ In the _Drosophila pseudoobscura / persimilis_ species complex, chromosomal inve
 suppress crossovers not only inside the inversion but also close to it (within 1–2 Mb)”
 ([[Machado, Haselkorn and Noor (2007)|biblio/17179068])].
 
+The overall rate of breakage in the _D. melanogaster_ / _D. yakuba_ lineage
+calculated by ([[Ranz and coll., 2007|bilbio/17550304]]) is 0.0183/Mb/Myr.  The
+authors “propose that the presence of inverted duplications associated with
+inversion breakpoint regions is the result of staggered breaks, either
+isochromatid or chromatid, and that this, rather than ectopic exchange between
+inverted repetitive sequences, is the prevalent mechanism for the generation of
+inversions in the melanogaster species group”. 
+
 [[Stefansson and coll, 2005||biblio/15654335]] found an inversion in the human
 genome and calculated that it appeared ~3 million years ago, before the
 speciation of _Homo sapiens_.  Low-copy repeated sequences were found in the

DNA methylation genes
diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 29e4869a..475eb22c 100644
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -268,6 +268,8 @@ Genes and pathways
    [[Albalat, Martí-Solans and Cañestro, 2012|biblio/22389042]]).
    It has Dnmt2, but this is a tRNA methyltransferase and it was later renamed Trdmt1 accordingly.
    [[Jurkowski and Jeltsch (2011)|bilbio/22140515]] did not find Dnmt2 in O. dioica.
+ - _O. dioica_ lost CDCA7, HELLS, UHRF1 and other genes related to DNA methylation
+   ([[Funabiki and coll., 2023|biblio/10.1101_2023.01.30.526367v1]]).
  - CYP1 family genes and their regulator AhR are not detectable
    ([[Yadetie et al, 2012|biblio/22300585]]).
  - No olfactory receptors have been found in _Oikopleura_ nor in _Ciona_

good morning
diff --git a/biblio/10.1101_2023.01.30.526367v1.mdwn b/biblio/10.1101_2023.01.30.526367v1.mdwn
new file mode 100644
index 00000000..7931c3f3
--- /dev/null
+++ b/biblio/10.1101_2023.01.30.526367v1.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Coevolution of the CDCA7-HELLS ICF-related nucleosome remodeling complex and DNA methyltransferases"]]
+[[!tag Oikopleura]]
+
+Hironori Funabiki, Isabel E. Wassing, Qingyuan Jia, Ji-Dung Luo, Thomas Carroll
+
+bioRxiv 2023.01.30.526367 doi:10.1101/2023.01.30.526367
+
+Coevolution of the CDCA7-HELLS ICF-related nucleosome remodeling complex and DNA methyltransferases
+
+[[!doi 10.1101/2023.01.30.526367v1 desc="In addition to DNA methyltransferases, _Oikopleura dioica_ has also lost “SNF2 family ATPase HELLS” and “its activator subunit CDCA7”, as well as the DNMT1 activator UHRF1."]]

Café
diff --git a/biblio/10411506.mdwn b/biblio/10411506.mdwn
new file mode 100644
index 00000000..01e4b47a
--- /dev/null
+++ b/biblio/10411506.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Generation of a widespread Drosophila inversion by a transposable element."]]
+[[!tag variants]]
+
+Cáceres M, Ranz JM, Barbadilla A, Long M, Ruiz A.
+
+Science. 1999 Jul 16;285(5426):415-8. doi:10.1126/science.285.5426.415
+
+Generation of a widespread Drosophila inversion by a transposable element.
+
+[[!pmid 10411506 desc="Target Site Duplication (TSD) sites were exchanged through the recombination of two transposons inserted in opposite directions."]]
diff --git a/tags/variants.mdwn b/tags/variants.mdwn
index 5562a25e..5bdf6ae5 100644
--- a/tags/variants.mdwn
+++ b/tags/variants.mdwn
@@ -38,6 +38,10 @@ supported by [[Steinberg and coll in 2012|biblio/22751100]].
 Ectopic recombination of a Galileo element may have caused a recent large-scale
 inversion in _D. buzzati_ ([[Delprat and coll, 2009|biblio/19936241]]).
 
+Flanking an inversion in _D. buzzati_ between two _Galileo_ elements, the
+exchange of Target-Site Duplication sequences was observed by Cáceres and
+coll., [[(1999|biblio/10411506]]). 
+
 [[Kent and coll., 2003|biblio/14500911]] reported 2 inversions per Mbp in
 human/mouse comparisons, median length 814. 
 

Mention AXTCHAIN
diff --git a/tags/alignment.mdwn b/tags/alignment.mdwn
index 6e7a6619..89b1ef03 100644
--- a/tags/alignment.mdwn
+++ b/tags/alignment.mdwn
@@ -2,6 +2,11 @@
 
 _Work in progress_
 
+## Chains and nets
+
+Kent and coll. ([[2003|biblio/14500911]]) computed _chained alignments_ with
+the AXTCHAIN program.
+
 ## LAST
 
 see [[LAST]] for a detailed bibliography.

progressive cactus
diff --git a/biblio/33177663.mdwn b/biblio/33177663.mdwn
new file mode 100644
index 00000000..a709cf61
--- /dev/null
+++ b/biblio/33177663.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Progressive Cactus is a multiple-genome aligner for the thousand-genome era."]]
+[[!tag genome alignment]]
+
+Armstrong J, Hickey G, Diekhans M, Fiddes IT, Novak AM, Deran A, Fang Q, Xie D, Feng S, Stiller J, Genereux D, Johnson J, Marinescu VD, Alföldi J, Harris RS, Lindblad-Toh K, Haussler D, Karlsson E, Jarvis ED, Zhang G, Paten B.
+
+Nature. 2020 Nov;587(7833):246-251. doi: 10.1038/s41586-020-2871-y
+
+Progressive Cactus is a multiple-genome aligner for the thousand-genome era
+
+[[!pmid 33177663 desc="Aligns with cactus 2~5 genomes, in- and out-group, and reconstitutes an ancestral genome.  Recurses the phylogenetic tree progressively.  A ‘best-hit-filtering’ step is added to catch duplications that are not seen in the outgroups.  Also runs a step ‘removing recoverable chains’ to allow for corrections and mitigate error propagation.  Aligning 600 amniotes took ~2 months."]]
diff --git a/tags/alignment.mdwn b/tags/alignment.mdwn
index 2bbe4c8b..6e7a6619 100644
--- a/tags/alignment.mdwn
+++ b/tags/alignment.mdwn
@@ -8,8 +8,12 @@ see [[LAST]] for a detailed bibliography.
 
 ## Cactus
 
- - Paten and coll, ([[March 2011|biblio/21385048]]) describe _cactus_ graphs
+ - Paten and coll., ([[March 2011|biblio/21385048]]) describe _cactus_ graphs
    where nodes are sets of adjascencies and edges are aligned blocks of
    sequences.  A genome can be represented as path in these graphs.
 
+ - Armstrong and coll. ([[2020|biblio/33177663]]) describe _progressive cactus_,
+   an iterative approach where ancestral genomes are reconstituted using 2-5 pairs
+   of in- and out-group comparisons, and then progressively aligned to each other.
+
 [[!inline pages="tagged(alignment)" limit=0]]

Re-fix
diff --git a/biblio/34934012.mdwn b/biblio/34934012.mdwn
index 31d7f286..08757e06 100644
--- a/biblio/34934012.mdwn
+++ b/biblio/34934012.mdwn
@@ -7,4 +7,4 @@ Proc Natl Acad Sci U S A. 2022 Jan 4;119(1):e2113075119. doi:10.1073/pnas.211307
 
 AnchorWave: Sensitive alignment of genomes with high sequence diversity, extensive structural polymorphism, and whole-genome duplication. 
 
-[[!pmid 34934012 desc="Maps a transcriptome to its reference genome.  2) Extracts "anchor" coding sequences.  3) Searches for homologous sequences in the query genome.  4) Realigns the sequences between homologous anchors.  The so-called comparison to LAST is actually a comparison with LAST + AxtChain, that is: it does not use last-split."]]
+[[!pmid 34934012 desc="Maps a transcriptome to its reference genome.  2) Extracts “anchor” coding sequences.  3) Searches for homologous sequences in the query genome.  4) Realigns the sequences between homologous anchors.  The so-called comparison to LAST is actually a comparison with LAST + AxtChain, that is: it does not use last-split."]]

Fix
diff --git a/biblio/34934012.mdwn b/biblio/34934012.mdwn
index cdbb799c..31d7f286 100644
--- a/biblio/34934012.mdwn
+++ b/biblio/34934012.mdwn
@@ -7,4 +7,4 @@ Proc Natl Acad Sci U S A. 2022 Jan 4;119(1):e2113075119. doi:10.1073/pnas.211307
 
 AnchorWave: Sensitive alignment of genomes with high sequence diversity, extensive structural polymorphism, and whole-genome duplication. 
 
-[[!pmid desc=""1) Maps a transcriptome to its reference genome.  2) Extracts "anchor" coding sequences.  3) Searches for homologous sequences in the query genome.  4) Realigns the sequences between homologous anchors.  The so-called comparison to LAST is actually a comparison with LAST + AxtChain, that is: it does not use last-split.]]
+[[!pmid 34934012 desc="Maps a transcriptome to its reference genome.  2) Extracts "anchor" coding sequences.  3) Searches for homologous sequences in the query genome.  4) Realigns the sequences between homologous anchors.  The so-called comparison to LAST is actually a comparison with LAST + AxtChain, that is: it does not use last-split."]]

Cactus
diff --git a/biblio/21385048.mdwn b/biblio/21385048.mdwn
new file mode 100644
index 00000000..77e98664
--- /dev/null
+++ b/biblio/21385048.mdwn
@@ -0,0 +1,13 @@
+[[!meta title="Cactus graphs for genome comparisons."]]
+[[!tag genome alignment]]
+
+Paten B, Diekhans M, Earl D, John JS, Ma J, Suh B, Haussler D.
+
+J Comput Biol. 2011 Mar;18(3):469-81. doi: 10.1089/cmb.2010.0252
+
+Cactus graphs for genome comparisons.
+
+[[!pmid 21385048 desc="Alignments are transformed in sequence graphs where
+nodes are connected by either alignment blocks or adjascencies between blocks,
+and this graph is progressively transformed in a cactus graph where the nodes
+are sets of adjascencies connected together without crossing a block."]]
diff --git a/tags/alignment.mdwn b/tags/alignment.mdwn
index 795e5919..2bbe4c8b 100644
--- a/tags/alignment.mdwn
+++ b/tags/alignment.mdwn
@@ -1,4 +1,15 @@
 [[!meta title="pages tagged alignment"]]
 
-[[!inline pages="tagged(alignment)" actions="no" archive="yes"
-feedshow=10]]
+_Work in progress_
+
+## LAST
+
+see [[LAST]] for a detailed bibliography.
+
+## Cactus
+
+ - Paten and coll, ([[March 2011|biblio/21385048]]) describe _cactus_ graphs
+   where nodes are sets of adjascencies and edges are aligned blocks of
+   sequences.  A genome can be represented as path in these graphs.
+
+[[!inline pages="tagged(alignment)" limit=0]]

Merge branch 'master' of ssh://charles-plessy-org.branchable.com
Work In Progress from last year.
diff --git a/biblio/20331767.mdwn b/biblio/20331767.mdwn
new file mode 100644
index 00000000..6782ce0a
--- /dev/null
+++ b/biblio/20331767.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water."]]
+[[!tag eDNA]]
+
+Stoeck T, Bass D, Nebel M, Christen R, Jones MD, Breiner HW, Richards TA
+
+Mol Ecol. 2010 Mar;19 Suppl 1:21-31. doi:10.1111/j.1365-294X.2009.04480.x
+
+Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water.
+
+[[!pmid 20331767 desc="The TAReuk454FWD1–TAReukREV3 pair (V4) detected more unique tags than the 1391F–EukB pair (V9)."]]
diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
index f15996b4..5b46ea4f 100644
--- a/tags/eDNA.mdwn
+++ b/tags/eDNA.mdwn
@@ -11,6 +11,8 @@
 
  - A Nextflow pipeline: eDNAFlow [[Mousavi‐Derazmahalleh and coll., 2021|biblio/10.1111_1755-0998.13356]].
 
+ - Universal or eukaryote-biased 18S primers targetting the V9 hypervariable region: [[Amaral-Zettler|biblio/19633714]]
+
 ### Published primers
 
 ```
@@ -22,11 +24,25 @@ GGAGCTGGAATTACCGC
 CCCTGCCHTTTGTACACAC
 >18S_1389F universal 10.1371/journal.pone.0006372
 TTGTACACACCGCCC
+>18S_1391F 10.1111/j.1365-294X.2009.04480.x (Lane 1991), S. cerevisiae NCBI GenBank nucleotide database accession # U53879 position 1629-1644)
+GTACACACCGCCCGTC
+>EukB 10.1111/j.1365-294X.2009.04480.x (Medlin et al. 1988), S. cerevisiae position 1774-1797). 
+TGATCCTTCTGCAGGTTCACCTAC
 >18S_1510R eukaryotic 10.1371/journal.pone.0006372
 CCTTCYGCAGGTTCACCTAC
 ```
 https://doi.org/10.1371/journal.pone.0073935
 
+
+V4
+```
+>TAReuk454FWD1 (S. cerevisiae position 565-584)
+CCAGCA(G/C)C(C/T)GCGGTAATTCC
+>TAReukREV3 (S. cerevisiae position 964-981)
+ACTTTCGTTCTTGATYRA
+```
+
+
 ```
 >UroCox1-244 F 10.2108/zsj.26.564
 CATTTWTTTTGATTWTTTRGWCATCCNGA

mdwn
diff --git "a/Debian/debi\303\242neries/markdown.en.po" "b/Debian/debi\303\242neries/markdown.en.po"
index 4e96336f..40808c68 100644
--- "a/Debian/debi\303\242neries/markdown.en.po"
+++ "b/Debian/debi\303\242neries/markdown.en.po"
@@ -3,40 +3,38 @@
 # This file is distributed under the same license as the PACKAGE package.
 # FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
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-#, fuzzy
 msgid ""
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-"Project-Id-Version: PACKAGE VERSION\n"
+"Project-Id-Version: \n"
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-"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
-"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
-"Language-Team: LANGUAGE <LL@li.org>\n"
-"Language: \n"
+"PO-Revision-Date: 2023-01-08 09:59+0900\n"
+"Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
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+"Last-Translator: \n"
+"Language-Team: \n"
+"X-Generator: Poedit 2.4.2\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta date=\"Sun, 08 Jan 2023 09:18:53 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta date=\"Sun, 08 Jan 2023 09:18:53 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta updated=\"Sun, 08 Jan 2023 09:18:53 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta updated=\"Sun, 08 Jan 2023 09:18:53 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!tag Debian]]\n"
-msgstr ""
+msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
 #, no-wrap
-msgid ""
-"[[!meta title=\"Quelqu'un pourrait-il patcher Firefox pour afficher du "
-"Markdown ?\"]]\n"
-msgstr ""
+msgid "[[!meta title=\"Quelqu'un pourrait-il patcher Firefox pour afficher du Markdown ?\"]]\n"
+msgstr "[[!meta title=\"Could somebody patch Firefox to display Markdown files?\"]]\n"
 
 #. type: Plain text
 msgid ""
@@ -45,7 +43,11 @@ msgid ""
 "comme un fichier texte.  Dans le ticket [1319262](), il est proposé "
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 msgstr ""
+"When Firefox receives a file with media type _text/markdown_, it prompts "
+"the user to download it, while other browsers display it as plain text.  "
+"In the ticket [1319262](), it is proposed to display Markdown files by "
+"default, but there needs a patch…"
 
 #. type: Plain text
 msgid "[1319262]: https://bugzilla.mozilla.org/show_bug.cgi?id=1319262"
-msgstr ""
+msgstr "[1319262]: https://bugzilla.mozilla.org/show_bug.cgi?id=1319262"

updated PO files
diff --git "a/Debian/debi\303\242neries/markdown.en.po" "b/Debian/debi\303\242neries/markdown.en.po"
new file mode 100644
index 00000000..4e96336f
--- /dev/null
+++ "b/Debian/debi\303\242neries/markdown.en.po"
@@ -0,0 +1,51 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2023-01-08 00:53+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Sun, 08 Jan 2023 09:18:53 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Sun, 08 Jan 2023 09:18:53 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"[[!meta title=\"Quelqu'un pourrait-il patcher Firefox pour afficher du "
+"Markdown ?\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Quand Firefox reçoit un fichier dont le type média est _text/markdown_, il "
+"le propose au téléchargement, alors que les autres navigateurs l'affichent "
+"comme un fichier texte.  Dans le ticket [1319262](), il est proposé "
+"d'afficher les fichiers Markdown par défaut.  Mais il faudrait un patch…"
+msgstr ""
+
+#. type: Plain text
+msgid "[1319262]: https://bugzilla.mozilla.org/show_bug.cgi?id=1319262"
+msgstr ""

Mdwn
diff --git "a/Debian/debi\303\242neries/markdown.mdwn" "b/Debian/debi\303\242neries/markdown.mdwn"
new file mode 100644
index 00000000..7bd4cc65
--- /dev/null
+++ "b/Debian/debi\303\242neries/markdown.mdwn"
@@ -0,0 +1,12 @@
+[[!meta date="Sun, 08 Jan 2023 09:18:53 +0900"]]
+[[!meta updated="Sun, 08 Jan 2023 09:18:53 +0900"]]
+[[!tag Debian]]
+
+[[!meta title="Quelqu'un pourrait-il patcher Firefox pour afficher du Markdown ?"]]
+
+Quand Firefox reçoit un fichier dont le type média est _text/markdown_, il le
+propose au téléchargement, alors que les autres navigateurs l'affichent comme
+un fichier texte.  Dans le ticket [1319262](), il est proposé d'afficher les
+fichiers Markdown par défaut.  Mais il faudrait un patch…
+
+[1319262]: https://bugzilla.mozilla.org/show_bug.cgi?id=1319262

Café
diff --git a/biblio/5456129.mdwn b/biblio/5456129.mdwn
index 104086ee..f5899b0a 100644
--- a/biblio/5456129.mdwn
+++ b/biblio/5456129.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="The diagram, a method for comparing sequences. Its use with amino acid and nucleotide sequences."]]
-[[!tag software method alignment]]
+[[!tag Oxford_plot]]
 
 Gibbs AJ, McIntyre GA.
 
diff --git a/tags/Oxford_plot.mdwn b/tags/Oxford_plot.mdwn
index 39072e90..10e755e8 100644
--- a/tags/Oxford_plot.mdwn
+++ b/tags/Oxford_plot.mdwn
@@ -1,4 +1,19 @@
 [[!meta title="pages tagged Oxford plot"]]
 
-[[!inline pages="tagged(Oxford_plot)" actions="no" archive="yes"
-feedshow=10]]
+## Why are the dot-plots of sequence comparisons also called “Oxford” plots?
+
+*work in progress*
+
+Wikipedia credits [[Gibbs and McIntyre (1970)|biblio/5456129]] for the
+invention of dot-plot graphs to compare two biological sequences.  Gibbs and
+McIntyre are from the University of Canberra, but the dot-plots are sometimes
+called “Oxford” plots or grids.  Is Wikipedia missing a reference?  I screened
+the papers citing Gibbs and McIntyre, and found that [[Maize and Lenk
+(1981)]|biblio/6801656]], from the NIH, also cite other papers: 1) [[Tinoco,
+Uhlenbeck and Levine (1971)|biblio/4927725]] from the University of Berkeley,
+that shows a base pairing matrix, 2) Fitch (1969) (PMID 5364927) from the
+University of Winsconsin, with no dot plot, and McLachlan (1970) (PMID 5167087)
+from the University of Cambridge, with a sequence comparison matrix for
+two proteins.
+
+[[!inline pages="tagged(Oxford_plot)" limit=0]]

creating tag page tags/Oxford_plot
diff --git a/tags/Oxford_plot.mdwn b/tags/Oxford_plot.mdwn
new file mode 100644
index 00000000..39072e90
--- /dev/null
+++ b/tags/Oxford_plot.mdwn
@@ -0,0 +1,4 @@
+[[!meta title="pages tagged Oxford plot"]]
+
+[[!inline pages="tagged(Oxford_plot)" actions="no" archive="yes"
+feedshow=10]]

Café
diff --git a/biblio/4927725.mdwn b/biblio/4927725.mdwn
new file mode 100644
index 00000000..c2ab013f
--- /dev/null
+++ b/biblio/4927725.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Estimation of secondary structure in ribonucleic acids."]]
+[[!tag Oxford_plot]]
+
+Tinoco I Jr, Uhlenbeck OC, Levine MD.
+
+Nature. 1971 Apr 9;230(5293):362-7. doi:10.1038/230362a0
+
+Estimation of secondary structure in ribonucleic acids.
+
+[[!pmid 4927725 desc="The base pairing matrix in figure 2 looks like an ”Oxford” plot."]]

Café
diff --git a/biblio/5456129.mdwn b/biblio/5456129.mdwn
new file mode 100644
index 00000000..104086ee
--- /dev/null
+++ b/biblio/5456129.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="The diagram, a method for comparing sequences. Its use with amino acid and nucleotide sequences."]]
+[[!tag software method alignment]]
+
+Gibbs AJ, McIntyre GA.
+
+Eur J Biochem. 1970 Sep;16(1):1-11. doi:10.1111/j.1432-1033.1970.tb01046.x
+
+The diagram, a method for comparing sequences. Its use with amino acid and nucleotide sequences.
+
+[[!pmid 5456129 desc="First description of the “Oxford” plot?"]]

en attendant
diff --git a/biblio/29506021.mdwn b/biblio/29506021.mdwn
new file mode 100644
index 00000000..e3cb72ad
--- /dev/null
+++ b/biblio/29506021.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Updating the 97% identity threshold for 16S ribosomal RNA OTUs."]]
+[[!tag eDNA]]
+
+Edgar RC.
+
+Bioinformatics. 2018 Jul 15;34(14):2371-2375. doi:10.1093/bioinformatics/bty113
+
+Updating the 97% identity threshold for 16S ribosomal RNA OTUs.
+
+[[!pmid 29506021 desc="Following a benchmark on V4 regions of the 16S rRNA, proposes to raise the threshold to 99% or to just use sequence variants (also called ZOTUS, Zero-radius OTUs."]]
diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
index f15996b4..10df54d7 100644
--- a/tags/eDNA.mdwn
+++ b/tags/eDNA.mdwn
@@ -11,6 +11,9 @@
 
  - A Nextflow pipeline: eDNAFlow [[Mousavi‐Derazmahalleh and coll., 2021|biblio/10.1111_1755-0998.13356]].
 
+ - Edgar ([[2018|biblio/29506021]] proposes to raise the threshold to 99% or to
+   just use sequence variants (also called ZOTUS, Zero-radius OTUs. 
+
 ### Published primers
 
 ```

Café
diff --git a/biblio/31112549.mdwn b/biblio/31112549.mdwn
new file mode 100644
index 00000000..7f8e38b9
--- /dev/null
+++ b/biblio/31112549.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Extensive loss of cell-cycle and DNA repair genes in an ancient lineage of bipolar budding yeasts."]]
+[[!tag yeast repair]]
+
+Steenwyk JL, Opulente DA, Kominek J, Shen XX, Zhou X, Labella AL, Bradley NP, Eichman BF, Čadež N, Libkind D, DeVirgilio J, Hulfachor AB, Kurtzman CP, Hittinger CT, Rokas A.
+
+PLoS Biol. 2019 May 21;17(5):e3000255. doi:10.1371/journal.pbio.3000255
+
+Extensive loss of cell-cycle and DNA repair genes in an ancient lineage of bipolar budding yeasts.
+
+[[!pmid 31112549 desc="“Compared to other budding yeasts in the subphylum _Saccharomycotina_, we noticed that a lineage in the genus _Hanseniaspora_ exhibited very high evolutionary rates, low Guanine–Cytosine (GC) content, small genome sizes, and lower gene numbers. [...] Our phylogenomic analyses identify two Hanseniaspora lineages, a faster-evolving lineage (FEL), which began diversifying approximately 87 million years ago (mya), and a slower-evolving lineage (SEL), which began diversifying approximately 54 mya.”"]]

yeast
diff --git a/biblio/36334587.mdwn b/biblio/36334587.mdwn
new file mode 100644
index 00000000..58d71954
--- /dev/null
+++ b/biblio/36334587.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Contrasting modes of macro and microsynteny evolution in a eukaryotic subphylum."]]
+[[!tag yeast synteny]]
+
+Li Y, Liu H, Steenwyk JL, LaBella AL, Harrison MC, Groenewald M, Zhou X, Shen XX, Zhao T, Hittinger CT, Rokas A.
+
+Curr Biol. 2022 Dec 19;32(24):5335-5343.e4. doi:10.1016/j.cub.2022.10.025
+
+Contrasting modes of macro and microsynteny evolution in a eukaryotic subphylum.
+
+[[!pmid 36334587 desc="“conservation index is calculated by counting the number of one-to-one orthologous gene pairs whose genes are in homologous chromosomes/scaffolds and dividing it by the number of one-to-one orthologs whose genes reside in non-homologous chromosomes/scaffolds” “we found a faster decay of macrosynteny conservation compared with filamentous fungi and animals, which is corroborated by findings of rapid chromosome structure evolution in budding yeasts” “at the small-scale gene-level of organization, we identified both deeply conserved and lineage-specific instances of conservation of microsynteny across budding yeast genomes.”"]]
diff --git a/tags/synteny.mdwn b/tags/synteny.mdwn
index bfe0bfdb..d9fd0d0f 100644
--- a/tags/synteny.mdwn
+++ b/tags/synteny.mdwn
@@ -57,6 +57,9 @@ phenomenon “mesosynteny”.
 Squid chromosomes still have synteny with scallop, but gene order is scrambled
 ([[Albertin and coll., 2022|biblio/35508532]]).
 
+At equal evolutionary distance, yeast microsynteny is lower than in animals,
+but higher than in plants ([[Li and coll., 2022|biblio/36334587]]).
+
 ### Ancestral karyotpyes
 
  - The ancestral mammalian genome has 30 chromosomes ([[Zhou and coll., 2021|biblio/33408411]]).

Café
diff --git a/biblio/35348662.mdwn b/biblio/35348662.mdwn
new file mode 100644
index 00000000..5ad15c7b
--- /dev/null
+++ b/biblio/35348662.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Chromosome-Level Reference Genomes for Two Strains of Caenorhabditis briggsae: An Improved Platform for Comparative Genomics."]]
+[[!tag nematode genome assembly]]
+
+Stevens L, Moya ND, Tanny RE, Gibson SB, Tracey A, Na H, Chitrakar R, Dekker J, Walhout AJM, Baugh LR, Andersen EC.
+
+Genome Biol Evol. 2022 Apr 10;14(4):evac042. doi:10.1093/gbe/evac042
+
+Chromosome-Level Reference Genomes for Two Strains of Caenorhabditis briggsae: An Improved Platform for Comparative Genomics.
+
+[[!pmid 35348662 desc="“the genomes of _C. elegans_ and _C. briggsae_ are more highly rearranged than their outcrossing sister species, _C. inopinata_ and _C. nigoni_ (17.1% of neighboring genes are rearranged in the selfers compared with 15.0% in the outcrossers)”"]]
diff --git a/tags/nematode.mdwn b/tags/nematode.mdwn
index 1d494f1b..001fd69c 100644
--- a/tags/nematode.mdwn
+++ b/tags/nematode.mdwn
@@ -53,4 +53,9 @@
    133 kb that are present in germ line but absent in somatic cells ([[de la
    Rosa and coll., 2021|biblio/33561231]]).
 
+ - “the genomes of _C. elegans_ and _C. briggsae_ are more highly rearranged
+   than their outcrossing sister species, _C. inopinata_ and _C. nigoni_ (17.1%
+   of neighboring genes are rearranged in the selfers compared with 15.0% in the
+   outcrossers)” ([[Stevens and coll., 2022|biblio/35348662]]).
+
 [[!inline pages="tagged(nematode)" limit=0]]

Café
diff --git a/biblio/33168940.mdwn b/biblio/33168940.mdwn
new file mode 100644
index 00000000..9a136950
--- /dev/null
+++ b/biblio/33168940.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Genomic and transcriptomic variation defines the chromosome-scale assembly of Haemonchus contortus, a model gastrointestinal worm."]]
+[[!tag nematode]]
+
+Doyle SR, Tracey A, Laing R, Holroyd N, Bartley D, Bazant W, Beasley H, Beech R, Britton C, Brooks K, Chaudhry U, Maitland K, Martinelli A, Noonan JD, Paulini M, Quail MA, Redman E, Rodgers FH, Sallé G, Shabbir MZ, Sankaranarayanan G, Wit J, Howe KL, Sargison N, Devaney E, Berriman M, Gilleard JS, Cotton JA.
+
+Commun Biol. 2020 Nov 9;3(1):656. doi:10.1038/s42003-020-01377-3
+
+Genomic and transcriptomic variation defines the chromosome-scale assembly of _Haemonchus contortus_, a model gastrointestinal worm.
+
+[[!pmid 33168940 desc="“80% of 7,361 one-to-one orthologous genes are shared on syntenic chromosomes between [_H. contortus_ and _C. elegans_]”  “The distance between ortholog pairs [...] is correlated up to ~100 kbp [...], but is largely lost above 100 kbp ”"]]

café
diff --git a/biblio/33561231.mdwn b/biblio/33561231.mdwn
new file mode 100644
index 00000000..df05d6ec
--- /dev/null
+++ b/biblio/33561231.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="A telomere-to-telomere assembly of Oscheius tipulae and the evolution of rhabditid nematode chromosomes."]]
+[[!tag nematode genome]]
+
+Gonzalez de la Rosa PM, Thomson M, Trivedi U, Tracey A, Tandonnet S, Blaxter M.
+
+G3 (Bethesda). 2021 Jan 18;11(1):jkaa020. doi:10.1093/g3journal/jkaa020
+
+A telomere-to-telomere assembly of Oscheius tipulae and the evolution of rhabditid nematode chromosomes.
+
+[[!pmid 33561231 desc="“subtelomeric extensions rang[ing] from 4 to 133 kb” present in germ line but absent in somatic cells."]]
diff --git a/tags/nematode.mdwn b/tags/nematode.mdwn
index 8d95f5bb..1d494f1b 100644
--- a/tags/nematode.mdwn
+++ b/tags/nematode.mdwn
@@ -49,4 +49,8 @@
    The Hi-C contact map shows strong interaction between the centre of all chromosomes
    ([[Sun and coll., 2020|biblio/33093047]]).
 
+ - In _Oscheius tipulae_, there are subtelomeric extensions ranging from 4 to
+   133 kb that are present in germ line but absent in somatic cells ([[de la
+   Rosa and coll., 2021|biblio/33561231]]).
+
 [[!inline pages="tagged(nematode)" limit=0]]

Merci PoEdit !
diff --git "a/Debian/debi\303\242neries/grosmot.en.po" "b/Debian/debi\303\242neries/grosmot.en.po"
index b0ab8398..66f89022 100644
--- "a/Debian/debi\303\242neries/grosmot.en.po"
+++ "b/Debian/debi\303\242neries/grosmot.en.po"
@@ -7,7 +7,7 @@ msgid ""
 msgstr ""
 "Project-Id-Version: \n"
 "POT-Creation-Date: 2022-12-16 13:24+0000\n"
-"PO-Revision-Date: 2022-12-16 22:31+0900\n"
+"PO-Revision-Date: 2022-12-16 22:35+0900\n"
 "Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
@@ -55,7 +55,7 @@ msgstr "I found it in:"
 
 #. type: Bullet: ' - '
 msgid "le code source de XEmacs ;"
-msgstr "The source code of XEmacs;"
+msgstr "the source code of XEmacs;"
 
 #. type: Bullet: ' - '
 msgid "une liste de gros mots pour policer les conversations dans BZFlag ;"
@@ -67,17 +67,17 @@ msgstr "the random sentence generator PolyGen;"
 
 #. type: Bullet: ' - '
 msgid "le code source du jeu de plateau Tagua ;"
-msgstr "The source code of the board game Tagua;"
+msgstr "the source code of the board game Tagua;"
 
 #. type: Bullet: ' - '
 msgid ""
 "une base de données d'épigrammes vulguaires pour la plateforme éducative "
 "WIMS ;"
-msgstr "A database of offensive fortunes for the educative platform WIMS;"
+msgstr "a database of offensive fortunes for the educative platform WIMS;"
 
 #. type: Bullet: ' - '
 msgid "le jeu de mots croisés parololottero ;"
-msgstr "The crossword game parolottero;"
+msgstr "the crossword game parolottero;"
 
 #. type: Bullet: ' - '
 msgid ""
@@ -98,7 +98,7 @@ msgstr "a source code comment aimed at somebody called Wolf;"
 
 #. type: Bullet: ' - '
 msgid "a collection of rude gestures in the xwrists package."
-msgstr "a collection of rude gestures in the xwrists package;"
+msgstr "a collection of rude gestures in the xwrists package."
 
 #. type: Plain text
 msgid ""

vafa
diff --git "a/Debian/debi\303\242neries/grosmot.en.po" "b/Debian/debi\303\242neries/grosmot.en.po"
index 2aac30ca..b0ab8398 100644
--- "a/Debian/debi\303\242neries/grosmot.en.po"
+++ "b/Debian/debi\303\242neries/grosmot.en.po"
@@ -3,38 +3,38 @@
 # This file is distributed under the same license as the PACKAGE package.
 # FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
 #
-#, fuzzy
 msgid ""
 msgstr ""
-"Project-Id-Version: PACKAGE VERSION\n"
+"Project-Id-Version: \n"
 "POT-Creation-Date: 2022-12-16 13:24+0000\n"
-"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
-"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
-"Language-Team: LANGUAGE <LL@li.org>\n"
-"Language: \n"
+"PO-Revision-Date: 2022-12-16 22:31+0900\n"
+"Language: en\n"
 "MIME-Version: 1.0\n"
 "Content-Type: text/plain; charset=UTF-8\n"
 "Content-Transfer-Encoding: 8bit\n"
+"Last-Translator: \n"
+"Language-Team: \n"
+"X-Generator: Poedit 2.4.2\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta date=\"Fri, 16 Dec 2022 22:00:03 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta date=\"Fri, 16 Dec 2022 22:00:03 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta updated=\"Fri, 16 Dec 2022 22:00:03 +0900\"]]\n"
-msgstr ""
+msgstr "[[!meta updated=\"Fri, 16 Dec 2022 22:00:03 +0900\"]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!tag Debian]]\n"
-msgstr ""
+msgstr "[[!tag Debian]]\n"
 
 #. type: Plain text
 #, no-wrap
 msgid "[[!meta title=\"Les grots mots dans Debian.\"]]\n"
-msgstr ""
+msgstr "[[!meta title=\"Bad words in Debian.\"]]\n"
 
 #. type: Plain text
 msgid ""
@@ -44,59 +44,66 @@ msgid ""
 "ensuite utilisé <http://codesearch.debian.net> pour étudier plus en détail "
 "son emploi."
 msgstr ""
+"A discussion on the _debian-project_ mailing list caught my attention to an "
+"Italian word meaning something like “would you be so kind to please go "
+"somewhere else?”, but in a more direct and vulgar manner. I then used  "
+"<http://codesearch.debian.net> to study its usage more in detail."
 
 #. type: Plain text
 msgid "Je l'ai trouvé dans :"
-msgstr ""
+msgstr "I found it in:"
 
 #. type: Bullet: ' - '
 msgid "le code source de XEmacs ;"
-msgstr ""
+msgstr "The source code of XEmacs;"
 
 #. type: Bullet: ' - '
 msgid "une liste de gros mots pour policer les conversations dans BZFlag ;"
-msgstr ""
+msgstr "a list of bad words to filter conversations in BZflag;"
 
 #. type: Bullet: ' - '
 msgid "le générateur aléatoire de phrases PolyGen ;"
-msgstr ""
+msgstr "the random sentence generator PolyGen;"
 
 #. type: Bullet: ' - '
 msgid "le code source du jeu de plateau Tagua ;"
-msgstr ""
+msgstr "The source code of the board game Tagua;"
 
 #. type: Bullet: ' - '
 msgid ""
 "une base de données d'épigrammes vulguaires pour la plateforme éducative "
 "WIMS ;"
-msgstr ""
+msgstr "A database of offensive fortunes for the educative platform WIMS;"
 
 #. type: Bullet: ' - '
 msgid "le jeu de mots croisés parololottero ;"
-msgstr ""
+msgstr "The crossword game parolottero;"
 
 #. type: Bullet: ' - '
 msgid ""
 "une base de données d'épigrammes vulguaires pour messages de bienvenue ou "
 "signatures de courriels ;"
 msgstr ""
+"a database of offensive fortunes for login screens and email signatures;"
 
 #. type: Bullet: ' - '
 msgid "des listes de mots de passes trop fréquents ;"
-msgstr ""
+msgstr "a list of (too) frequent passwords;"
 
 #. type: Bullet: ' - '
 msgid ""
 "un commentaire destiné à un déonmmé Wolf dans le code source d'un autre "
 "programme ;"
-msgstr ""
+msgstr "a source code comment aimed at somebody called Wolf;"
 
 #. type: Bullet: ' - '
 msgid "a collection of rude gestures in the xwrists package."
-msgstr ""
+msgstr "a collection of rude gestures in the xwrists package;"
 
 #. type: Plain text
 msgid ""
 "Ce fut une promenade rafraîchissante et récréactive dans l'univers des "
 "paquets Debian."
 msgstr ""
+"That was a refreshing and pleasant recreation in the Debian package "
+"universe."

updated PO files
diff --git "a/Debian/debi\303\242neries/grosmot.en.po" "b/Debian/debi\303\242neries/grosmot.en.po"
new file mode 100644
index 00000000..2aac30ca
--- /dev/null
+++ "b/Debian/debi\303\242neries/grosmot.en.po"
@@ -0,0 +1,102 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2022-12-16 13:24+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"Fri, 16 Dec 2022 22:00:03 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta updated=\"Fri, 16 Dec 2022 22:00:03 +0900\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Debian]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Les grots mots dans Debian.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Une conversation sur la list _debian-project_ a attiré mon attention sur un "
+"mot italien signifiant quelque chose comme « auriez-vous la gentillesse "
+"d'aller voir ailleurs ? », mais en version plus directe et vulgaire.  J'ai "
+"ensuite utilisé <http://codesearch.debian.net> pour étudier plus en détail "
+"son emploi."
+msgstr ""
+
+#. type: Plain text
+msgid "Je l'ai trouvé dans :"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "le code source de XEmacs ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "une liste de gros mots pour policer les conversations dans BZFlag ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "le générateur aléatoire de phrases PolyGen ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "le code source du jeu de plateau Tagua ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"une base de données d'épigrammes vulguaires pour la plateforme éducative "
+"WIMS ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "le jeu de mots croisés parololottero ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"une base de données d'épigrammes vulguaires pour messages de bienvenue ou "
+"signatures de courriels ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "des listes de mots de passes trop fréquents ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"un commentaire destiné à un déonmmé Wolf dans le code source d'un autre "
+"programme ;"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "a collection of rude gestures in the xwrists package."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Ce fut une promenade rafraîchissante et récréactive dans l'univers des "
+"paquets Debian."
+msgstr ""