Chez Charles/ tags/ pages tagged enzyme
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Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase.
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Machine-driven parameter screen of biochemical reactions.
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Characterization of Agrobacterium tumefaciens DNA ligases C and D.
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Error-Speed Correlations in Biopolymer Synthesis.
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Biodegradation of waste PET: A sustainable solution for dealing with plastic pollution.
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Tn5 transposase and tagmentation procedures for massively scaled sequencing projects.
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A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining.
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Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA.
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TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.
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Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli.
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Type III restriction enzymes need two inversely oriented recognition sites for DNA cleavage.
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T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis
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The synthesis of oligodeoxyribonucleotides using RNA ligase.
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Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein.
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Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization.
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In vitro selection of restriction endonucleases by in vitro compartmentalization.
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Psychrophilic proteases dramatically reduce single cell RNA-seq artifacts: A molecular atlas of kidney development.
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Simple cDNA normalization using kamchatka crab duplex-specific nuclease.
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Purification and properties of bacteriophage T4-induced RNA ligase.
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Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
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Donor activation in the T4 RNA ligase reaction.
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Reactions at the termini of tRNA with T4 RNA ligase.
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Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.
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Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.
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Archaebacterial DNA polymerases tightly bind uracil-containing DNA.
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The mitochondrial RNA ligase from Leishmania tarentolae can join RNA molecules bridged by a complementary RNA.
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Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase.
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Alkaline phosphatase from the Antarctic strain TAB5. Properties and psychrophilic adaptations.
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Engineering the properties of a cold active enzyme through rational redesign of the active site.
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Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation.
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Purification of histidine-tagged T4 RNA ligase from E. coli.
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Bacteriophage T4 RNA ligase 2 (gp24.1) exemplifies a family of RNA ligases found in all phylogenetic domains.
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Structure-function analysis of T4 RNA ligase 2.
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Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
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Structure and mechanism of RNA ligase.
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Characterization of bacteriophage KVP40 and T4 RNA ligase 2.
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Practical and general synthesis of 5'-adenylated RNA (5'-AppRNA).
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RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2.
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Dual mechanisms whereby a broken RNA end assists the catalysis of its repair by T4 RNA ligase 2.
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Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties.
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Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins.
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Novel 3'-ribonuclease and 3'-phosphatase activities of the bacterial non-homologous end-joining protein, DNA ligase D.
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The wild-type Schizosaccharomyces pombe mat1 imprint consists of two ribonucleotides.
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A second, non-canonical RNA-dependent RNA polymerase in SARS coronavirus.
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XRCC4:DNA ligase IV can ligate incompatible DNA ends and can ligate across gaps.
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Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase.
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Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers.
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Haplotype-resolved whole-genome sequencing by contiguity-preserving transposition and combinatorial indexing.
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Template-independent ligation of single-stranded DNA by T4 DNA ligase.
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Novel 5'/3'RACE Method for Amplification and Determination of Single-Stranded RNAs Through Double-Stranded RNA (dsRNA) Intermediates.
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Efficient and quantitative high-throughput tRNA sequencing.
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Conditions for the selective synthesis of DNA complementary to template RNA.
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Aprataxin resolves adenylated RNA-DNA junctions to maintain genome integrity.
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Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing.
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Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells.
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Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing.
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Endonuclease V cleaves at inosines in RNA.
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Human endonuclease V is a ribonuclease specific for inosine-containing RNA.
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Apparent non-canonical trans-splicing is generated by reverse transcriptase in vitro.
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An end-healing enzyme from Clostridium thermocellum with 5' kinase, 2',3' phosphatase, and adenylyltransferase activities.
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Reconstituting bacterial RNA repair and modification in vitro.
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Bacterial Hen1 is a 3' terminal RNA ribose 2'-O-methyltransferase component of a bacterial RNA repair cassette.
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Synthesis, base pairing properties and trans-lesion synthesis by reverse transcriptases of oligoribonucleotides containing the oxidatively damaged base 5-hydroxycytidine.
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Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease.
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Type III restriction enzymes cleave DNA by long-range interaction between sites in both head-to-head and tail-to-tail inverted repeat
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Type III restriction endonucleases translocate DNA in a reaction driven by recognition site-specific ATP hydrolysis.
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Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
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Genetic and biochemical analysis of the functional domains of yeast tRNA ligase.
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HSPC117 is the essential subunit of a human tRNA splicing ligase complex.
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Reverse transcriptase template switching and false alternative transcripts.
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Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis.
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A novel phosphodiesterase from cultured tobacco cells.
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Increased yield of PCR products by addition of T4 gene 32 protein to the SMART PCR cDNA synthesis system.
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A highly efficient method for long-chain cDNA synthesis using trehalose and betaine.
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Extra-long first-strand cDNA synthesis.
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2′,3′-cyclic phosphates and nontemplated nucleotides at the 3′ end of spliceosomal U6 small nuclear RNAs.
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Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
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Identification of a deoxyribonuclease I inhibitor from a phage-peptide library.
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Exogenous AdoMet and its analogue sinefungin differentially influence DNA cleavage by R.EcoP15I--usefulness in SAGE.
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tRNAHis guanylyltransferase catalyzes a 3′-5′ polymerization reaction that is distinct from G₋₁ addition.
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Dynamic assembly of primers on nucleic acid templates.
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Unique substrate spectrum and PCR application of Nanoarchaeum equitans family B DNA polymerase.
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Structural explanation for the role of Mn2+ in the activity of phi6 RNA-dependent RNA polymerase.
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Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer.
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Characterization of Moloney Murine Leukaemia Virus/Avian Myeloblastosis Virus Chimeric Reverse Transcriptases.
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Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus.
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Functional Characterization and Modulation of the DNA Cleavage Efficiency of Type III Restriction Endonuclease EcoP15I in Its Interaction with Two Sites in the DNA Target.
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The MmeI family: type II restriction-modification enzymes that employ single-strand modification for host protection.
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Importance of 5′-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
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Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
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End labeling of enzymatically decapped mRNA.
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Sequence-specific cleavage of RNA by Type II restriction enzymes.
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Branchiostoma floridae has separate healing and sealing enzymes for 5'-phosphate RNA ligation.
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Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase.
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Thermostabilization and thermoactivation of thermolabile enzyme by trehalose and its application for the synthesis of full length cDNA.
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Characterization of RNA strand displacement synthesis by Moloney murine leukemia virus reverse transcriptase.
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Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display.
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ScriptCap™ m7G Capping System.
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