Baris Y, Taylor MRG, Aria V, Yeeles JTP.

Nature. 2022 Jun;606(7912):204-210. doi:10.1038/s41586-022-04759-1

Fast and efficient DNA replication with purified human proteins.

A minimal human replisome comprising only CMG, Polε and PCNA has a speed of 1.67 kb min−1. Addition of AND-1, TIM–TIPIN, CLASPIN and CTF18–RFC increases that speed to 4.41 kb min−1, “which is over twice the mean rate and towards the upper limit of observed fork rate distributions measured in human cells”.

Japaridze A, Gogou C, Kerssemakers JWJ, Nguyen HM, Dekker C.

Nat Commun. 2020 Jun 19;11(1):3109. doi:10.1038/s41467-020-16946-7

Direct observation of independently moving replisomes in Escherichia coli.

Replisomes were visualised with labelling the the β-clamp (DnaN). The chromosome was labelled by attaching a mYpet fluorescent tag to the HU protein. A temperature-sensitive DnaC allele was used to synchronise the cell cycles and to elongate the cells. A MreB mutation was used to generate wider cells.