Baris Y, Taylor MRG, Aria V, Yeeles JTP.

Nature. 2022 Jun;606(7912):204-210. doi:10.1038/s41586-022-04759-1

Fast and efficient DNA replication with purified human proteins.

A minimal human replisome comprising only CMG, Polε and PCNA has a speed of 1.67 kb min−1. Addition of AND-1, TIM–TIPIN, CLASPIN and CTF18–RFC increases that speed to 4.41 kb min−1, “which is over twice the mean rate and towards the upper limit of observed fork rate distributions measured in human cells”.