Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku

Commun Biol. 2020 Nov 6;3(1):652. doi:10.1038/s42003-020-01373-7

Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis.

Primary cell culture of fibroblasts and lymphocytes. Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”. In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide.

Burnette M, Brito-Robinson T, Li J, Zartman J.

Mol Biosyst. 2014 Oct;10(10):2713-23. doi:10.1039/c4mb00155a

An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines.

“Using this approach we developed an intermediate “ZO Fortified” medium, made up of ZO media supplemented with insulin (5 μg mL−1), trehalose (26.4 mM), l-alanyl-l-glutamine (ala-gln, 12 μM), and l-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (A2P, 0.08 μM), with pH adjusted to 6.75.” 410 compounds were screened. “All of the repeated positive hits were polyamines (spermine, spermidine, and putrescine)”

Mitsuhashi J.

In Vitro Cell Dev Biol Anim. 1998 Sep;34(8):619-21. doi:10.1007/s11626-996-0007-9

Polyamine as a growth promoter for cultured insect cells.

At least one of putrescine, spermidine and spermine at 2 µg per 1000 ml need to be in the culture medium of NIH-SaPe-4 cells (from the fly Boettcherisca peregrina) could be passaged more than 10 times.

In Vitro Cell Dev Biol Anim. 2019 Mar;55(3):149-158. doi:10.1007/s11626-018-00317-0

Munroe S, Sandoval K, Martens DE, Sipkema D, Pomponi SA.

Genetic algorithm as an optimization tool for the development of sponge cell culture media

4 generations of 30 experiments each screening the concentrations of all amino acids.

Marteijn RC, Jurrius O, Dhont J, de Gooijer CD, Tramper J, Martens DE.

Biotechnol Bioeng. 2003 Feb 5;81(3):269-78. doi:10.1002/bit.10465

Optimization of a feed medium for fed-batch culture of insect cells using a genetic algorithm

11 parameters encoded in 5 bits each. 4 generations of 20 experiments each. Helicoverpa zea cell-line HzAm1.

Kawamura K, Nishitsuji K, Shoguchi E, Fujiwara S, Satoh N.

Mar Biotechnol (NY). 2021 Apr 26. doi:10.1007/s10126-021-10031-w

Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.

Dissociation with “a mixture of trypsin, EDTA, and collagenase”. “Treatment for 1–2 h at 25–28 °C did not complete cell dissociation, but after 3–4 h, only single cells without debris were found in the culture dish.” “[The modular protease] plasmin (2 μg/mL) was effective in maintaining dissociated cells in culture for more than 2 weeks, during which a large number of a new type of cell appeared in the culture dish.” “Aliquots of polyclonal cell population were harvested from the primary 24-well culture plate, diluted fivefold with growth medium containing plasmin, and dispensed into 96-well plates (100 μL/well). Cells in clumps proliferated with a doubling time of 2–3 days.”

Schneider I.

J Embryol Exp Morphol. 1972 Apr;27(2):353-65.

Cell lines derived from late embryonic stages of Drosophila melanogaster.

“At least one line is derived from imaginal disc cells.” As reported for other insects, cell spheres appeared in the cultures after 2–3 weeks. At each passage, spheres are trypsinised or simple teased apart.

Echalier G, Ohanessian A.

C R Acad Hebd Seances Acad Sci D. 1969 Mar 31;268(13):1771-3.

https://gallica.bnf.fr/ark:/12148/bpt6k62969219/f623.image

Spontaneous transformation after multiple months of culture.

Lin YC, Boone M, Meuris L, Lemmens I, Van Roy N, Soete A, Reumers J, Moisse M, Plaisance S, Drmanac R, Chen J, Speleman F, Lambrechts D, Van de Peer Y, Tavernier J, Callewaert N.

Nat Commun. 2014 Sep 3;5:4767. doi:10.1038/ncomms5767

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.

“a culture of 293 cells should be considered as an entire 'population' of individual cells with different chromosomal structure makeup”

Frey O, Misun PM, Fluri DA, Hengstler JG, Hierlemann A.

Nat Commun. 2014 Jun 30;5:4250. doi: 10.1038/ncomms5250

Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis.

“Maximal flow velocities appear at the liquid–air interface”. Uses open capillary channels and valves.

Lindskog U, Lundgren B, Billig D, Lindner E.

Dev Biol Stand. 1987;66:307-13.

Alternatives for harvesting cells grown on microcarriers: effects on subsequent attachment and growth.

Köster et al. suggested to encapsulate cells attached to single beads.