Burnette M, Brito-Robinson T, Li J, Zartman J.

Mol Biosyst. 2014 Oct;10(10):2713-23. doi:10.1039/c4mb00155a

An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines.

“Using this approach we developed an intermediate “ZO Fortified” medium, made up of ZO media supplemented with insulin (5 μg mL−1), trehalose (26.4 mM), l-alanyl-l-glutamine (ala-gln, 12 μM), and l-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (A2P, 0.08 μM), with pH adjusted to 6.75.” 410 compounds were screened. “All of the repeated positive hits were polyamines (spermine, spermidine, and putrescine)”