Mizuno Y, Carninci P, Okazaki Y, Tateno M, Kawai J, Amanuma H, Muramatsu M, Hayashizaki Y.

Nucleic Acids Res. 1999 Mar 1;27(5):1345-9.

Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display.

Reverse transcriptase can prime when the last two bases mismatch.