Chez Charles/ biblio/ Donor activation in the T4 RNA ligase reaction. Edit RecentChanges History Preferences Branchable McLaughlin LW, Piel N, Graeser E. Biochemistry. 1985 Jan 15;24(2):267-73. Donor activation in the T4 RNA ligase reaction. Adenylated RNA is a better substrate for T4 RNA ligase. Adenylation efficiency after 6h: C >> U ~ A > G.