Nucleic Acids Res. 1979 Sep 25;7(2):453-64.

Hinton DM, Gumport RI.

The synthesis of oligodeoxyribonucleotides using RNA ligase.

A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield.