Brooks AN, Hughes AL, Clauder-Münster S, Mitchell LA, Boeke JD, Steinmetz LM.

Science. 2022 Mar 4;375(6584):1000-1005. doi: 10.1126/science.abg0162

Transcriptional neighborhoods regulate transcript isoform lengths and expression levels.

“a synthetic yeast genome (Sc2.0) was designed to encode a Cre-dependent system known as synthetic chromosome rearrangement and modification by LoxP-mediated evolution (SCRaMbLE), which can generate stochastic genomic rearrangements on demand directly in its genome. These rearrangements occur at 34 base pair (bp)–loxPsym sites inserted 3 bp downstream of the stop codon of all nonessential CDSs.”