Kawamura K, Nishitsuji K, Shoguchi E, Fujiwara S, Satoh N.

Mar Biotechnol (NY). 2021 Apr 26. doi:10.1007/s10126-021-10031-w

Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.

Dissociation with “a mixture of trypsin, EDTA, and collagenase”. “Treatment for 1–2 h at 25–28 °C did not complete cell dissociation, but after 3–4 h, only single cells without debris were found in the culture dish.” “[The modular protease] plasmin (2 μg/mL) was effective in maintaining dissociated cells in culture for more than 2 weeks, during which a large number of a new type of cell appeared in the culture dish.” “Aliquots of polyclonal cell population were harvested from the primary 24-well culture plate, diluted fivefold with growth medium containing plasmin, and dispensed into 96-well plates (100 μL/well). Cells in clumps proliferated with a doubling time of 2–3 days.”