Fuqua T, Jordan J, van Breugel ME, Halavatyi A, Tischer C, Polidoro P, Abe N, Tsai A, Mann RS, Stern DL, Crocker J.

Nature. 2020 Nov;587(7833):235-239. doi:10.1038/s41586-020-2816-5

Dense and pleiotropic regulatory information in a developmental enhancer

Study of the E3N enhancer of the shavenbaby (svb) gene. Immunohistochemistry automated with liquid handling robots. “sequence conservation is not an accurate predictor of the quantitative roles of individual sites in the E3N enhancer” “most mutations in E3N led to changes in transcriptional outputs, suggesting that regulatory information is distributed densely within this enhancer”