Srinivasan P, Smolke CD.

Nature. 2020 Sep;585(7826):614-619. doi:10.1038/s41586-020-2650-9

Biosynthesis of medicinal tropane alkaloids in yeast.

“Our final strain comprises 34 chromosomal modifications (26 genes, 8 gene disruptions), resulting in an integrated whole-cell system that expresses enzymes and transporters in diverse sub-cellular locations (cytosol, mitochondria, peroxisome, vacuole, ER and vacuolar membranes)” Screened a plant RNA-seq to discover one of the enzymes. Protein sequence needed modifications for proper trans-compartment transport and maturation in the yeast cells.