Robert A. Policastro, R. Taylor Raborn, Volker P. Brendel and Gabriel E. Zentner

Genome Res. 2020 Jun;30(6):910-923. doi:10.1101/gr.261545.120

Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq

Terminator-treated RNA reverse-transcribed with random N5 primers. The (biotinylated) TSO is added in the last 5 min of the RT (~40 µM final). The TSO carries a P5 linker and the RTP a P7 (indexed) linker. Follows a standard PCR and sequencing. The YR motif is detected in yeast and K562 cells. High amounts of rRNA remains in yeast