Japaridze A, Gogou C, Kerssemakers JWJ, Nguyen HM, Dekker C.

Nat Commun. 2020 Jun 19;11(1):3109. doi:10.1038/s41467-020-16946-7

Direct observation of independently moving replisomes in Escherichia coli.

Replisomes were visualised with labelling the the β-clamp (DnaN). The chromosome was labelled by attaching a mYpet fluorescent tag to the HU protein. A temperature-sensitive DnaC allele was used to synchronise the cell cycles and to elongate the cells. A MreB mutation was used to generate wider cells.