Ola M, O'Brien CE, Coughlan AY, Ma Q, Donovan PD, Wolfe KH, Butler G.

Genome Res. 2020 May;30(5):684-696. doi:10.1101/gr.257816.119.

Polymorphic Centromere Locations in the Pathogenic Yeast Candida parapsilosis

HA epitope introduced into centromeric histone H3 (Cse4) using CRISPR-Cas9 editing. ChIP-seq then identified centromeres. “The species C. parapsilosis is therefore polymorphic for centromere location on two chromosomes. The centromere relocations are associated with a transition from a structured (IR) format to a format with no obvious structure or sequence dependence, within a single species. On Chromosome 5, it is likely that the centromeres on both copies of this chromosome have moved to a new location. It is possible that C. parapsilosis 90-137 is heterozygous at CEN1, with Cse4 at the expected location on one copy of Chromosome 1 and at a new location on the other copy.” “The C. parapsilosis neocentromeres are formed at regions that are transcribed, and transcription is known to facilitate centromere activity in S. cerevisiae”