Muramoto N, Oda A, Tanaka H, Nakamura T, Kugou K, Suda K, Kobayashi A, Yoneda S, Ikeuchi A, Sugimoto H, Kondo S, Ohto C, Shibata T, Mitsukawa N, Ohta K.

Nat Commun. 2018 May 18;9(1):1995. doi:10.1038/s41467-018-04256-y. PMID: 29777105; PMCID: PMC5959919.

Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks.

TaqI-aided genome rearrangement (“TAQing system”) in yeast and Arabidopsis, using a heat-sensitive (>37°C) restriction enzyme (Taq-I, cleaving at TCGA) to introduce double-strand breaks and mutations such as gene conversions or translocations.