Nucleic Acids Res. 2018 Jul 27;46(13):e79. doi:10.1093/nar/gky303

Potapov V, Ong JL, Langhorst BW, Bilotti K, Cahoon D, Canton B, Knight TF, Evans TC Jr, Lohman GJS.

A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining.

TNA overhangs are very inefficient to ligate. However ANT are not harder to ligate than similar mismatches. GC-rich overhangs better tolerate mismatches than AT-rich ones. Increasing temperature reduced mismatched ligations. 5′-G mismatches are much better tolerated than 5′-T mismatches.