Development. 2017 Aug 29. pii: dev.151142. doi:10.1242/dev.151142

Adam M, Potter AS, Potter SS.

Psychrophilic proteases dramatically reduce single cell RNA-seq artifacts: A molecular atlas of kidney development.

“ a method for single-cell dissociation using a cold active protease from Bacillus licheniformis, which is a soil bacterium that can grow in a wide range of temperatures and has been isolated from Himalayan glaciers”