Science. 2017 May 5;356(6337):503-508. doi:10.1126/science.aag3260

Takahashi Y, Wu J, Suzuki K, Martinez-Redondo P, Li M, Liao HK, Wu MZ, Hernández-Benítez R, Hishida T, Shokhirev MN, Esteban CR, Sancho-Martinez I, Belmonte JCI.

Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells.

“both interruption of the methylation-blocking machinery and re- pression of transcriptional activity are necessary and sufficient to trigger de novo CGI methylation in PSCs”