Nucleic Acids Res. 2016 Dec 9. pii: gkw1242. doi:10.1093/nar/gkw1242

Arguel MJ, LeBrigand K, Paquet A, Ruiz García S, Zaragosi LE, Barbry P, Waldmann R.

A cost effective 5' selective single cell transcriptome profiling approach with improved UMI design.

Backload of barcodes between RT and PCR. Changing the terminal base of the template-switching oligonucleotide from RNA to LNA did not increase performance.