Picher ÁJ, Budeus B, Wafzig O, Krüger C, García-Gómez S, Martínez-Jiménez MI, Díaz-Talavera A, Weber D, Blanco L, Schneider A.

Nat Commun. 2016 Nov 29;7:13296. doi:10.1038/ncomms13296

TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.

Isothermal amplification for 3 h at 30 °C using a mixture of TthPrimPol for priming and and Φ29DNApol for extension. Target DNA was first denatured in an alkaline buffer.