Oz-Gleenberg I, Herschhorn A, Hizi A.

Nucleic Acids Res. 2011 Feb;39(3):1042-53. doi: 10.1093/nar/gkq786

Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis.

AT-rich cDNA tails are not triggering template-switching well. CC are the strongest. Mn₂⁺ potentiates template switching for MMLV. dGTP stabilises the clamp between the enzyme and the nucleic acids.