Proc Natl Acad Sci U S A. 1977 Jul;74(7):2734-8.
Shimotohno K, Kodama Y, Hashimoto J, Miura KI.
Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
In this study, TAP is used in 0.1 M NaAc (pH 5.5), 5 mM EDTA, during 90 min at 30℃.