Spiess AN, Ivell R.

Anal Biochem. 2002 Feb 15;301(2):168-74 doi:10.1006/abio.2001.5474

A highly efficient method for long-chain cDNA synthesis using trehalose and betaine.

Effects of trehalose and betaine on reverse-transcriptase are additive. Betain improves reverse-transcription by decreasing melting temperature of structured molecules.