Science. 2001 Dec 21;294(5551):2506 doi:10.1126/science.294.5551.2506

Seo HC, Kube M, Edvardsen RB, Jensen MF, Beck A, Spriet E, Gorsky G, Thompson EM, Lehrach H, Reinhardt R, Chourrout D.

Miniature genome in the marine chordate Oikopleura dioica.

Genome size estimated to 72 ± 13 Mb by DNA contents and comparison to Ciona. A large-scale shotgun sequencing of 128,386 short reads lead to a lower-bound estimate of 65 Mb and 15,000 genes.