Illumina flowcell adaptors
Information extracted from the supplemental information of ?Bentley et al., 2008 (Accurate whole human genome sequencing using reversible terminator chemistry, Nature 456(7218):53–9)
Single read flowcell
5´-PSTTTTTTTTTT-(diol)3-AATGATACGGCGACCACCGA-3´
5´-PSTTTTTTTTTTCAAGCAGAAGACGGCATACGA-3´
Paired read flowcell
5´-PS-TTTTTTTTTTAATGATACGGCGACCACCGAGAUCTACAC-3´ (U = 2-deoxyuridine)
5´-PS-TTTTTTTTTTCAAGCAGAAGACGGCATACGAGoxoAT-3´ (Goxo = 8-oxoguanine)
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A specific combination of dual index adaptors decreases the sensitivity of amplicon sequencing with the Illumina platform.
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High-throughput DNA sequencing errors are reduced by orders of magnitude using circle sequencing.
Method to cancel PCR and sequencing noise. Needs long reads. Suggested for TCR sequencing.
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A single-molecule long-read survey of the human transcriptome.
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60/5 min ratios “were highest for introns in a range of 300–400 nt”.
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Synthethised aRNAs, ligated adaptors and sequenced paired-end on HiSeq 2000
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Accurate whole-genome sequencing and haplotyping from 10 to 20 human cells.
Haplotype sequencing by pooling barcoded dillutions from the same sample.
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Single-molecule mechanical identification and sequencing.
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Age, microbiota, and T cells shape diverse individual IgA repertoires in the intestine.
β-fixed cells colonise hosts less efficiently than WT hosts.
Parallel study of antigen as self or non-self through transgenic expression.
Different receptoromes for regulatory and conventional T cells.
Estimates about one million different β chains in the blood.
Contains a description of sequencing by oligonucleotide ligation and detection (SOLiD).
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Genome sequencing in microfabricated high-density picolitre reactors.
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Bentley DR, many co-authors, and Smith AJ.
Accurate whole human genome sequencing using reversible terminator chemistry.
“found evidence of convergence, in which different individuals made the same antibody”
Does not use quality values, and recommends to discard redundant reads.
Basecalled RNA-seq reads to find SNPs and study allelic imbalance.
Most enhancer bound by P300 in the mouse embryonnic heart at E11.5 are not strongly conserved.
78 % of the recombinant antibodies generated from the most abundant sequences were antigen-specific.