Expired:
- ?Method for suppressing DNA fragment amplification during PCR (1996)
- Method for suppressing DNA fragment amplification during PCR (1998)
Some commercial kits contain dangerous clauses about the property of the products made with them.
The Clontech patent for 5'RACE. See also its obsolete counterpart, 5,962,271.
See also patent US5565340.
Describes the use of chaproning agents to protect the RT, of moderate excess of dNTPs to protect RNA from magnesium, and even of very small quantities of detergents to further increase the rate of full-length transcription. See also patents 6221599 and 6013488.