Adenylylation

Adenylylation is the addition of an AMP moiety to a phosphorylated end. In vitro, it is performed by ligases. See the ligase tag for details.

Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA.

Torchia C, Takagi Y, Ho CK.

Nucleic Acids Res. 2008 Nov;36(19):6218-27. doi:10.1093/nar/gkn602

Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA.

Used in the NEB's 5´ DNA Adenylation Kit. Lacks specificity for RNA and DNA. Optimal at 65 °C, inactivated at 75 °C, and mostly adenylylating at 37 °C.

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