Chez Charles/ tags/ pages tagged H3S10p

Histone 3 serine 10 is phosphorylated in mitosis and meiosis, see Prigent and Dmitrov (2003) for review.

Figure 1j of Ganot P1, Kallesøe T, Thompson EM (2007) shows H3S10p staining of meiotic chromosomes in π-conformation in mature oocytes.

The monoclonal antibody 9706S used in Søviknes and Glover (2008) stains the phosphorylated serine 10 on histone 3. Double-staining with BrdU in the notocord suggests adult cell proliferation.

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Functional specialization of Aurora kinase homologs during oogenic meiosis in the tunicate Oikopleura dioica.

Feng H, Thompson EM.

Front Cell Dev Biol. 2023 Dec 7;11:1323378. doi:10.3389/fcell.2023.1323378

Functional specialization of Aurora kinase homologs during oogenic meiosis in the tunicate Oikopleura dioica.

“a C-terminal GFP fusion [of OdAur1] was localized on centrosomes throughout mitosis” “a commercial phospho-Aurora antibody that can recognize the phosphorylated forms of both Aurora1 and Aurora2 in O. dioica [localised] on centrosomes and chromosomes in prophase and metaphase, and on centrosomes and the central spindle in anaphase” “This suggests that Aurora1 and Aurora2 in O. dioica represent the polar and equatorial forms of Aurora kinases”. “[Knockdown] suggests that Aurora1 is involved in promoting the progression of prometaphase I. In all the Aur2 KD oocytes, both H3-pS10 and H3-pS28 were absent, and chromosomes were decondensed, reminiscent of INCENPa knockdown phenotypes”

Continued growth and cell proliferation into adulthood in the notochord of the appendicularian Oikopleura dioica.

Søviknes AM, Glover JC.

Biol Bull. 2008 Feb;214(1):17-28. doi:10.2307/25066656

Continued growth and cell proliferation into adulthood in the notochord of the appendicularian Oikopleura dioica.

Actin stained with phalloidin, DNA stained with To-Pro-3, and BrdU stained with antibodies. Dual H2S10p and BrdU staining in the adult notochord suggest cell proliferation.

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Oocyte selection is concurrent with meiosis resumption in the coenocystic oogenesis of Oikopleura.

Ganot P, Moosmann-Schulmeister A, Thompson EM.

Dev Biol. 2008 Dec 15;324(2):266-76. doi:10.1016/j.ydbio.2008.09.016

Oocyte selection is concurrent with meiosis resumption in the coenocystic oogenesis of Oikopleura.

H3S28p signal grows becomes highest only in the subset of H2S10p-labeled oocytes that become selected for maturation. MAPK inhibition has a similar effect to microtubule destabilisation.

Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions.

Cell Cycle. 2019 Jul 15:1-20. doi:10.1080/15384101.2019.1634954

Feng H, Raasholm M, Moosmann A, Campsteijn C, Thompson EM.

Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions.

Components of the constitutive centromere-associated network (CCAN) at the inner kinetochore seem to be missing in the genome. H3T3p and H3S28p mark inner centromeric regions (single spots) and H3T11p mark outer centromeric regions (pairs of spots). Fluorescent microscopy pictures supporting a 2 × 3 karyotype.

Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.

Spada F, Chioda M, Thompson EM.

J Cell Biochem. 2005 Aug 1;95(5):885-901. doi:10.1002/jcb.20416

Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.

Knobs are also seem to be in H4K20me2. In endocycling cells, H4K20me2 tends to be stronger in BrdU-negative (short pulse) cells. CldU/IdU staining confirms that H4K20me2 decreases during the S phase of endocycling cells. In mitotically cycling cells, BrdU-low cells tended to be H4K20me2-weak. Strong H3S28p and H3S10p staining (M phase markers) correlated with strong H4K20me2 staining. In NIH3T3 cells, H4K20me2 peaks at M phase and then decreases from G1 to G2. H4K16Ac stainings were not reproducible across antibodies.

The cytoskeleton organizes germ nuclei with divergent fates and asynchronous cycles in a common cytoplasm during oogenesis in the chordate Oikopleura.

Ganot P, Kallesøe T, Thompson EM.

Dev Biol. 2007 Feb 15;302(2):577-90 doi:10.1016/j.ydbio.2006.10.022

The cytoskeleton organizes germ nuclei with divergent fates and asynchronous cycles in a common cytoplasm during oogenesis in the chordate Oikopleura.

In early meiotic nuclei, telomeres and nuclear pore complexes ”cluster in non-overlapping regions of the nuclear envelope”. During some stages of oocyte maturation, the telomeres of the endocycling nurse cells “clustered at the nuclear periphery, in a compartment with no detectable elongating RNA polymerase II”. Colchicine treatment induced early oocyte differenciation. Used rabbit polyclonal anti-histone H3 phospho-serine 10 (H3S10P) from Upstate, cat. num. 06-570.