Klebe RJ, Grant GM, Grant AM, Garcia MA, Giambernardi TA, Taylor GP.

Biotechniques. 1996 Dec;21(6):1094-100.

RT-PCR without RNA isolation.

Freeze-thaw method. 5 mM DTT partially protects the RNA for 1 h in absence of RNAse inhibitors. Hypertonic solutions may prevent the premature disruption of the cell and its lysosomes prior lysis.