Volloch VZ, Schweitzer B, Rits S.

DNA Cell Biol. 1995 Dec;14(12):991-6 doi:10.1089/dna.1995.14.991

Transcription of the 5'-terminal cap nucleotide by RNA-dependent DNA polymerase: possible involvement in retroviral reverse transcription.

Utilises a 5′ hairpin in the beta-globin mRNA to design a cDNA extension assay where the reverse-transcribed cap creates a terminal mismatch that inhibits extension. De-capping the RNA removes the inhibition. 40 mM Tris 8.3, 40 mM KCl, 5 mM MgCl2, 2 mM DTT. AMV, 45°C