Anal Biochem. 1986 Oct;158(1):171-8.

Tessier DC, Brousseau R, Vernet T.

Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.

ssDNA ligation. Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM.