Simon Henriet, Anne Elin Aasjord, Daniel Marc Chourrout

Front Zool. 2022 Oct 28;19(1):26. doi:10.1186/s12983-022-00471-y

Laboratory study of Fritillaria lifecycle reveals key morphogenetic events leading to genus-specific anatomy.

Possibly parasited by a Syndiniale from the genus Sphaeripara and Oodinium. Fed with Micromonas pusilla or Synechococcus sp in culture at a density between 2 and 8 × 107 depending on the developmental stage. 4 chromosomes are visible in oocytes by DAPI staining. Self-fertilization was never observed. In vitro fertilized oocytes frequently aborted development when left in vessels without water flow. Embryos hatch between 4 and 6 h at 15 °C. Cellulose-producing cells identified by staining with a carbohydrate-binding-module (CBM).