Weisman CM, Murray AW, Eddy SR.

Curr Biol. 2022 May 12:S0960-9822(22)00721-7. doi:10.1016/j.cub.2022.04.085

Mixing genome annotation methods in a comparative analysis inflates the apparent number of lineage-specific genes.

“In all but one of the eight cases in Figure 1, the increase is more than 2-fold, suggesting that the majority of lineage-specific genes inferred in heterogeneous annotations are artifacts of the heterogeneity.” “As expected, six-frame translation homology searches dramatically reduce the apparent number of lineage-specific genes.”