Jensen KB, Dredge BK, Toubia J, Jin X, Iadevaia V, Goodall GJ, Proud CG.

Nucleic Acids Res. 2021 Oct 11;49(18):e105. doi:10.1093/nar/gkab604

capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E-mRNA interactome.

eIF4E flagged via CRISPR/Cas9 gene editing. mRNAs were crosslinked to eIF4E and immunoprecipitated with anti-flag antibodies. First-strand cDNAs primed via a linker ligated in 3′. This method (capCLIP) was used to study the effect of rapamycin treatment and eIF4E phosphorylation.