Terekhov SS, Eliseev IE, Ovchinnikova LA, Kabilov MR, Prjibelski AD, Tupikin AE, Smirnov IV, Belogurov AA Jr, Severinov KV, Lomakin YA, Altman S, Gabibov AG.

Proc Natl Acad Sci U S A. 2020 Oct 21:202017138. doi:10.1073/pnas.2017138117.

Liquid drop of DNA libraries reveals total genome information.

“[...] either 3% of Abil EM 180 (A-oil) or a mixture of 4.5% Span 80/0.4% Tween 80/0.05% Triton X-100 (T-oil) was used [...] The homogeneity of the amplified DNA was much better in the case of A-oil, whereas ePCR in T-oil resulted in the formation of high-molecular-weight by-products [...] Thirty-five cycles of ePCR in A-oil were sufficient to reach saturation in the majority of the droplets. T-oil was less stable, displaying droplet coalescence after 25 cycles [...].” “Appearance of clear amplicon bands on electropherograms does not correlate with the uniformity of amplification during ePCR.” “ePCR clearly outperformed bulk PCR, reducing the number of reads with gross errors by twofold [and] resulted in a more uniform distribution of amplified sequences”