Chez Charles/ biblio/ Copolymerization of single-cell nucleic acids into balls of acrylamide gel.

[[!tag single_cell emulsion method]

Li S, Kendall J, Park S, Wang Z, Alexander J, Moffitt A, Ranade N, Danyko C, Gegenhuber B, Fischer S, Robinson BD, Lepor H, Tollkuhn J, Gillis J, Brouzes E, Krasnitz A, Levy D, Wigler M.

Genome Res. 2019 Nov 14. doi:10.1101/gr.253047.119

Copolymerization of single-cell nucleic acids into balls of acrylamide gel.

In a droplet, DNA or RNA from a single cell is annealed to a acrydite primer. An acrylamide gel is then polymerised before emulsion is broken. DNA strands are then polymerised. Barcoding is then done by split-and-pool method.