Bhardwaj V, Semplicio G, Erdogdu NU, Manke T, Akhtar A.

Nat Commun. 2019 Jul 30;10(1):3219. doi:10.1038/s41467-019-11115-x

MAPCap allows high-resolution detection and differential expression analysis of transcription start sites.

Reports that in their reverse-transcription reaction, G-addition is very low.

RT reaction in 20 µL: 10x Buffer 2 μL; dNTP (10 mM) 1 μL → 500 µM final; MgCl2 (25 mM) 4 μL → 5 mM final; DTT (0.1 M) 2 μL → 10 mM final; RNaseOUT (40 U/μl) 1 μL; SSIII (200 U/μL) 1 μL