[!tag genome assembly mosquito method purification]]

Sarah Kingan, Haynes Heaton, Juliana Cudini, Christine Lambert, Primo Baybayan, Brendan Galvin, Richard Durbin, Jonas Korlach, Mara Lawniczak

Genes (Basel). 2019 Jan 18;10(1). pii: E62. doi:10.3390/genes10010062

A High-Quality De Novo Genome Assembly from a Single Mosquito using PacBio Sequencing

Single individual ground with no twisting (only up-down movements). DNA extracted with Qiagen MagAttract HMW kit, gentle flipping and wide-bore tips. One contig was a bacterial genome, probably from gut flora. Two contigs contained multiple copies of the mitochondrial chromosome. Comparison with Sanger assembly corrected repeat compression.