Nat Methods. 2019 Jan;16(1):55-58. doi:10.1038/s41592-018-0258-x

Xu H, Fair BJ, Dwyer ZW, Gildea M, Pleiss JA.

Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing.

Specificity of RT priming can be increased by increasing temperature from 47 °C to 55 °C.