Nat Commun. 2018 Nov 30;9(1):5114. doi:10.1038/s41467-018-07641-9

Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S.

High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries.

Less than 0.2% of the genome pairs had an average nucleotide identity between 83% and 95%.