G3 (Bethesda). 2018 Aug 7. pii: g3.200160.2018. doi:10.1534/g3.118.200160

Miller DE, Staber C, Zeitlinger J, Hawley RS.

Highly Contiguous Genome Assemblies of 15 Drosophila Species Generated Using Nanopore Sequencing.

29× coverage and N50 of 4.4. Mb in average. A multiplexed NextSeq 500 run was used for polishing. Optimisation of Nanopore throughput by reorganising pore groups periodically, extracting high molecular weight DNA with phenol/chloroform extration, and using more DNA in the library preparation. Benchmark of various tools including minimap/miniasm and canu.