Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing.

G3 (Bethesda). 2018 Jul 17. pii: g3.200162.2018. doi:10.1534/g3.118.200162

Solares EA, Chakraborty M, Miller DE, Kalsow S, Hall K, Perera AG, Emerson JJ, Hawley RS.

Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing.

Hybrid de novo assembly (Nanopore / Illumina / Optical) of the Drosophila genome reaches a high (>98%) « BUSCO » score typical of high-quality mainstream reference assemblies. (BUSCO stands for « Benchmarking Universal Single-Copy Orthologs ».)