Development. 2018 Jul 11;145(13). pii: dev164640. doi:10.1242/dev.164640

Matsushima W, Herzog VA, Neumann T, Gapp K, Zuber J, Ameres SL, Miska EA.

SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting.

SLAMseq in tissue (SLAM-ITseq). Tissue-specific UPRT expression selectively incorporates 4-thiouracil in RNA. Alkylation with iodoacetamide (IAA) leads to G → A mutations during reverse transcription, which are detected and quantified during data analysis.