Sci Rep. 2018 Jun 14;8(1):9106. doi:10.1038/s41598-018-27509-8

López-Escardó D, Paps J, de Vargas C, Massana R, Ruiz-Trillo I, Del Campo J.

Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity.

28% of the RNA reads in the oxic micro/mesoplanktonic samples originate from tunicates, mostly appendicularian. rRNA sequences with no known homologs suggest a new group of tunicates, “MAME 1”. However, given very long branches in the phylogenetic tree, this finding needs to be confirmed.