Nat Commun. 2018 Feb 22;9(1):781. doi:10.1038/s41467-018-03149-4

Clark SJ, Argelaguet R, Kapourani CA, Stubbs TM, Lee HJ, Alda-Catalinas C, Krueger F, Sanguinetti G, Kelsey G, Marioni JC, Stegle O, Reik W.

scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells.

GpC methyltransferase need accessibiltiy to the native chromatin. RNA and DNA are split primor amplification. SMART-seq on RNA and bisulfite (scBS) on DNA. Throughput limited by the DNA/RNA separation.