Karst SM, Dueholm MS, McIlroy SJ, Kirkegaard RH, Nielsen PH, Albertsen M.

Nat Biotechnol. 2018 Feb;36(2):190-195. doi:10.1038/nbt.4045

Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias.

Sequences are flanked with UMIs. The library is split in two parts. The first is tagmented and sequenced as usual. The second is circularised, tagemented and sequenced. Thus, UMI pairing information is recovered and both ends can be assembled even if they are in separate tagments.