Chez Charles/ biblio/ Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning.

Biotechniques. 2017 Sep 1;63(3):125-130. doi:10.2144/000114588

Islam MN, Lee KW, Yim HS, Lee SH, Jung HC, Lee JH, Jeong JY.

Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning.

Use of T4 DNA polymerase at 50 °C as an exonuclease for generating sinngle-strand overhangs.